Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates

Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expr...
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Autor*in:	Seyedeh Sedigheh Hosseini [verfasserIn] Mohamad Hossein Yadegari [verfasserIn] Masoumeh Rajabibazl [verfasserIn] Ezzat Allah Ghaemi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba

Übergeordnetes Werk:	In: Iranian Journal of Microbiology - Tehran University of Medical Sciences, 2020, 8(2017), 6
Übergeordnetes Werk:	volume:8 ; year:2017 ; number:6

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ065005899

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520			\|a Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.
650		4	\|a Carvacrol
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author_variant	s s h ssh m h y mhy m r mr e a g eag
matchkey_str	article:20084447:2017----::niioyfetocrarlnhepesoosceeaprypoenss3nlcnzl
hierarchy_sort_str	2017
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allfields	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2 DE-627 ger DE-627 rakwb eng QR1-502 Seyedeh Sedigheh Hosseini verfasserin aut Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology Mohamad Hossein Yadegari verfasserin aut Masoumeh Rajabibazl verfasserin aut Ezzat Allah Ghaemi verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 8(2017), 6 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:8 year:2017 number:6 https://doaj.org/article/172a59f6ca254ead881fde093c7be2f2 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/1021 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 6
spelling	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2 DE-627 ger DE-627 rakwb eng QR1-502 Seyedeh Sedigheh Hosseini verfasserin aut Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology Mohamad Hossein Yadegari verfasserin aut Masoumeh Rajabibazl verfasserin aut Ezzat Allah Ghaemi verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 8(2017), 6 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:8 year:2017 number:6 https://doaj.org/article/172a59f6ca254ead881fde093c7be2f2 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/1021 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 6
allfields_unstemmed	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2 DE-627 ger DE-627 rakwb eng QR1-502 Seyedeh Sedigheh Hosseini verfasserin aut Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology Mohamad Hossein Yadegari verfasserin aut Masoumeh Rajabibazl verfasserin aut Ezzat Allah Ghaemi verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 8(2017), 6 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:8 year:2017 number:6 https://doaj.org/article/172a59f6ca254ead881fde093c7be2f2 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/1021 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 6
allfieldsGer	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2 DE-627 ger DE-627 rakwb eng QR1-502 Seyedeh Sedigheh Hosseini verfasserin aut Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology Mohamad Hossein Yadegari verfasserin aut Masoumeh Rajabibazl verfasserin aut Ezzat Allah Ghaemi verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 8(2017), 6 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:8 year:2017 number:6 https://doaj.org/article/172a59f6ca254ead881fde093c7be2f2 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/1021 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 6
allfieldsSound	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2 DE-627 ger DE-627 rakwb eng QR1-502 Seyedeh Sedigheh Hosseini verfasserin aut Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound. Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology Mohamad Hossein Yadegari verfasserin aut Masoumeh Rajabibazl verfasserin aut Ezzat Allah Ghaemi verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 8(2017), 6 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:8 year:2017 number:6 https://doaj.org/article/172a59f6ca254ead881fde093c7be2f2 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/1021 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017 6
language	English
source	In Iranian Journal of Microbiology 8(2017), 6 volume:8 year:2017 number:6
sourceStr	In Iranian Journal of Microbiology 8(2017), 6 volume:8 year:2017 number:6
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba Microbiology
isfreeaccess_bool	true
container_title	Iranian Journal of Microbiology
authorswithroles_txt_mv	Seyedeh Sedigheh Hosseini @@aut@@ Mohamad Hossein Yadegari @@aut@@ Masoumeh Rajabibazl @@aut@@ Ezzat Allah Ghaemi @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	688077226
id	DOAJ065005899
language_de	englisch
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callnumber-first	Q - Science
author	Seyedeh Sedigheh Hosseini
spellingShingle	Seyedeh Sedigheh Hosseini misc QR1-502 misc Carvacrol misc SAP1-3 misc Candida albicans misc Fluconazole-resistant misc Echinophora platyloba misc Microbiology Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
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topic_title	QR1-502 Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates Carvacrol SAP1-3 Candida albicans Fluconazole-resistant Echinophora platyloba
topic	misc QR1-502 misc Carvacrol misc SAP1-3 misc Candida albicans misc Fluconazole-resistant misc Echinophora platyloba misc Microbiology
topic_unstemmed	misc QR1-502 misc Carvacrol misc SAP1-3 misc Candida albicans misc Fluconazole-resistant misc Echinophora platyloba misc Microbiology
topic_browse	misc QR1-502 misc Carvacrol misc SAP1-3 misc Candida albicans misc Fluconazole-resistant misc Echinophora platyloba misc Microbiology
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title	Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
ctrlnum	(DE-627)DOAJ065005899 (DE-599)DOAJ172a59f6ca254ead881fde093c7be2f2
title_full	Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
author_sort	Seyedeh Sedigheh Hosseini
journal	Iranian Journal of Microbiology
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author_browse	Seyedeh Sedigheh Hosseini Mohamad Hossein Yadegari Masoumeh Rajabibazl Ezzat Allah Ghaemi
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author-letter	Seyedeh Sedigheh Hosseini
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title_sort	inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant candida albicans isolates
callnumber	QR1-502
title_auth	Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
abstract	Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.
abstractGer	Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.
abstract_unstemmed	Background and Objectives: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis ofCandida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatmentwith Echinophora platyloba extract, carvacrol and caspofungin drug. Materials and Methods: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. Canida albicansspecies were identified using phenotypic and genotyping methods. Spectrophotometry was used to investigate thepresence of SAP protein in the vaginal samples, and SDS-PAGE was used to confirm its protein composition. Real-timePCR was performed to ascertain the effects of subinhibitory concentrations of Echinophora platyloba extract, carvacrol andcaspofungin on the expression of SAP1-3 genes before and after treatment. Results: C. albicans was found as the abundant species (59.6%), and different amounts of SAP were present in all vaginalsamples, which were higher than Candida krusei strain. The protein composition of SAP in C. albicans samples was estimatedwith the approximate molecular weight of 45 kDa. mRNA levels of total SAP in FLU-resistant isolates (P=0.01) were morethan those of FLU-susceptible isolates (P=0.07). The findings indicated that carvacrol is effective in reduction of SAP1-3 expression with a particular effect against FLU-resistant isolates. Conclusion: Carvacrol contains an essential oil (carvacrol); therefore, it can be considered as an alternative effective antifungal compound.
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title_short	Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates
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Inhibitory effects of carvacrol on the expression of secreted aspartyl proteinases 1-3 in fluconazole-resistant Candida albicans isolates

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