Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis

<p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Kress-Bennett Jennifer M [verfasserIn] Ehrlich Garth D [verfasserIn] Bruno Ashley [verfasserIn] Post J Christopher [verfasserIn] Hu Fen Z [verfasserIn] Scott Thomas F [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2011

Übergeordnetes Werk:	In: BMC Neurology - BMC, 2003, 11(2011), 1, p 155
Übergeordnetes Werk:	volume:11 ; year:2011 ; number:1, p 155

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2377-11-155

Katalog-ID:	DOAJ065079604

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245	1	0	\|a Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
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520			\|a <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p<
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author_variant	k b j m kbjm e g d egd b a ba p j c pjc h f z hfz s t f stf
matchkey_str	article:14712377:2011----::rlmnrsuyramnwtitauclrnefrneaaeutiicesdeesfl22uspndcesdeesfl3s
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publishDate	2011
allfields	10.1186/1471-2377-11-155 doi (DE-627)DOAJ065079604 (DE-599)DOAJb1cf511c4ad14a4b9caaf53a6a2930a9 DE-627 ger DE-627 rakwb eng RC346-429 Kress-Bennett Jennifer M verfasserin aut Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Neurology. Diseases of the nervous system Ehrlich Garth D verfasserin aut Bruno Ashley verfasserin aut Post J Christopher verfasserin aut Hu Fen Z verfasserin aut Scott Thomas F verfasserin aut In BMC Neurology BMC, 2003 11(2011), 1, p 155 (DE-627)326643664 (DE-600)2041347-6 14712377 nnns volume:11 year:2011 number:1, p 155 https://doi.org/10.1186/1471-2377-11-155 kostenfrei https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 kostenfrei http://www.biomedcentral.com/1471-2377/11/155 kostenfrei https://doaj.org/toc/1471-2377 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 155
spelling	10.1186/1471-2377-11-155 doi (DE-627)DOAJ065079604 (DE-599)DOAJb1cf511c4ad14a4b9caaf53a6a2930a9 DE-627 ger DE-627 rakwb eng RC346-429 Kress-Bennett Jennifer M verfasserin aut Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Neurology. Diseases of the nervous system Ehrlich Garth D verfasserin aut Bruno Ashley verfasserin aut Post J Christopher verfasserin aut Hu Fen Z verfasserin aut Scott Thomas F verfasserin aut In BMC Neurology BMC, 2003 11(2011), 1, p 155 (DE-627)326643664 (DE-600)2041347-6 14712377 nnns volume:11 year:2011 number:1, p 155 https://doi.org/10.1186/1471-2377-11-155 kostenfrei https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 kostenfrei http://www.biomedcentral.com/1471-2377/11/155 kostenfrei https://doaj.org/toc/1471-2377 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 155
allfields_unstemmed	10.1186/1471-2377-11-155 doi (DE-627)DOAJ065079604 (DE-599)DOAJb1cf511c4ad14a4b9caaf53a6a2930a9 DE-627 ger DE-627 rakwb eng RC346-429 Kress-Bennett Jennifer M verfasserin aut Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Neurology. Diseases of the nervous system Ehrlich Garth D verfasserin aut Bruno Ashley verfasserin aut Post J Christopher verfasserin aut Hu Fen Z verfasserin aut Scott Thomas F verfasserin aut In BMC Neurology BMC, 2003 11(2011), 1, p 155 (DE-627)326643664 (DE-600)2041347-6 14712377 nnns volume:11 year:2011 number:1, p 155 https://doi.org/10.1186/1471-2377-11-155 kostenfrei https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 kostenfrei http://www.biomedcentral.com/1471-2377/11/155 kostenfrei https://doaj.org/toc/1471-2377 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 155
allfieldsGer	10.1186/1471-2377-11-155 doi (DE-627)DOAJ065079604 (DE-599)DOAJb1cf511c4ad14a4b9caaf53a6a2930a9 DE-627 ger DE-627 rakwb eng RC346-429 Kress-Bennett Jennifer M verfasserin aut Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Neurology. Diseases of the nervous system Ehrlich Garth D verfasserin aut Bruno Ashley verfasserin aut Post J Christopher verfasserin aut Hu Fen Z verfasserin aut Scott Thomas F verfasserin aut In BMC Neurology BMC, 2003 11(2011), 1, p 155 (DE-627)326643664 (DE-600)2041347-6 14712377 nnns volume:11 year:2011 number:1, p 155 https://doi.org/10.1186/1471-2377-11-155 kostenfrei https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 kostenfrei http://www.biomedcentral.com/1471-2377/11/155 kostenfrei https://doaj.org/toc/1471-2377 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 155
allfieldsSound	10.1186/1471-2377-11-155 doi (DE-627)DOAJ065079604 (DE-599)DOAJb1cf511c4ad14a4b9caaf53a6a2930a9 DE-627 ger DE-627 rakwb eng RC346-429 Kress-Bennett Jennifer M verfasserin aut Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p< Neurology. Diseases of the nervous system Ehrlich Garth D verfasserin aut Bruno Ashley verfasserin aut Post J Christopher verfasserin aut Hu Fen Z verfasserin aut Scott Thomas F verfasserin aut In BMC Neurology BMC, 2003 11(2011), 1, p 155 (DE-627)326643664 (DE-600)2041347-6 14712377 nnns volume:11 year:2011 number:1, p 155 https://doi.org/10.1186/1471-2377-11-155 kostenfrei https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 kostenfrei http://www.biomedcentral.com/1471-2377/11/155 kostenfrei https://doaj.org/toc/1471-2377 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2011 1, p 155
language	English
source	In BMC Neurology 11(2011), 1, p 155 volume:11 year:2011 number:1, p 155
sourceStr	In BMC Neurology 11(2011), 1, p 155 volume:11 year:2011 number:1, p 155
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Neurology. Diseases of the nervous system
isfreeaccess_bool	true
container_title	BMC Neurology
authorswithroles_txt_mv	Kress-Bennett Jennifer M @@aut@@ Ehrlich Garth D @@aut@@ Bruno Ashley @@aut@@ Post J Christopher @@aut@@ Hu Fen Z @@aut@@ Scott Thomas F @@aut@@
publishDateDaySort_date	2011-01-01T00:00:00Z
hierarchy_top_id	326643664
id	DOAJ065079604
language_de	englisch
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Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). 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callnumber-first	R - Medicine
author	Kress-Bennett Jennifer M
spellingShingle	Kress-Bennett Jennifer M misc RC346-429 misc Neurology. Diseases of the nervous system Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
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topic_title	RC346-429 Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
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title	Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
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title_full	Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
author_sort	Kress-Bennett Jennifer M
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author_browse	Kress-Bennett Jennifer M Ehrlich Garth D Bruno Ashley Post J Christopher Hu Fen Z Scott Thomas F
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author-letter	Kress-Bennett Jennifer M
doi_str_mv	10.1186/1471-2377-11-155
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title_sort	preliminary study: treatment with intramuscular interferon beta-1a results in increased levels of il-12rβ2<sup<+ </sup<and decreased levels of il23r<sup<+ </sup<cd4<sup<+ </sup<t - lymphocytes in multiple sclerosis
callnumber	RC346-429
title_auth	Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
abstract	<p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<There are a lack of biomarkers which can be used to predict clinical outcomes for multiple sclerosis (MS) patients receiving interferon beta (IFN-β). Thus the objective of this study was to characterize changes in CD4+ T-lymphocyte expression in an unbiased manner following initiation of intramuscular (IM) IFN-β-1a treatment, and then to verify those findings using marker-specific assays.</p< <p<Methods</p< <p<Peripheral blood specimens were collected from twenty MS patients before and after treatment with intramuscular (IM) IFN-β-1a and were used for isolation of mononuclear cells (PBMCs). mRNA expression patterns of negatively-selected CD4+ T-cells from the PBMCs were analyzed using microarray gene expression technology. IL-12 and IL-23 receptor levels on PBMC-derived CD4+ T-cells were analyzed by flow cytometry. The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). Thus, the changes in the numbers of CD4<sup<+ </sup<T-cells expressing these receptors in response to IFN-β-1a treatment may point to an important mechanism of action for this drug, but further large scale studies are needed to confirm these preliminary observations.</p<
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container_issue	1, p 155
title_short	Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis
url	https://doi.org/10.1186/1471-2377-11-155 https://doaj.org/article/b1cf511c4ad14a4b9caaf53a6a2930a9 http://www.biomedcentral.com/1471-2377/11/155 https://doaj.org/toc/1471-2377
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The phosphorylation status of Stat4 was measured by performing densitometry on western blots.</p< <p<Results</p< <p<Microarray analyses demonstrated that mRNA expression of the IL-12Rβ2 gene was uniformly up-regulated in response to IFN-β-1a treatment and was associated with an increased number of IL-12Rβ2<sup<+ </sup<CD4<sup<+ </sup<T-cells by flow cytometry in 4 of 6 patients. This finding was substantiated by demonstrating that Stat4 phosphorylation, a transcription factor for IL-12, was increased after treatment. Conversely, the number of IL-23R<sup<+ </sup<CD4<sup<+ </sup<T-cells was decreased following treatment.</p< <p<Conclusions</p< <p<The IL-12 receptor shares a common subunit, the IL-12Rβ2, with the IL-23 receptor. Both of these receptors have a probable role in regulating IL-17 and TH-17 cells, important mediators of inflammation in multiple sclerosis (MS). 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Preliminary study: Treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2<sup<+ </sup<and decreased levels of IL23R<sup<+ </sup<CD4<sup<+ </sup<T - Lymphocytes in multiple sclerosis

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