Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland

<p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MA...
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Autor*in:	Stephan Roger [verfasserIn] Corti Sabrina [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2002

Übergeordnetes Werk:	In: BMC Microbiology - BMC, 2003, 2(2002), 1, p 15
Übergeordnetes Werk:	volume:2 ; year:2002 ; number:1, p 15

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2180-2-15

Katalog-ID:	DOAJ065086732

Internformat


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allfields	10.1186/1471-2180-2-15 doi (DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198 DE-627 ger DE-627 rakwb eng QR1-502 Stephan Roger verfasserin aut Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p< Microbiology Corti Sabrina verfasserin aut In BMC Microbiology BMC, 2003 2(2002), 1, p 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:2 year:2002 number:1, p 15 https://doi.org/10.1186/1471-2180-2-15 kostenfrei https://doaj.org/article/388cc46b0bcd4233ba3971e75e422198 kostenfrei http://www.biomedcentral.com/1471-2180/2/15 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1, p 15
spelling	10.1186/1471-2180-2-15 doi (DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198 DE-627 ger DE-627 rakwb eng QR1-502 Stephan Roger verfasserin aut Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p< Microbiology Corti Sabrina verfasserin aut In BMC Microbiology BMC, 2003 2(2002), 1, p 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:2 year:2002 number:1, p 15 https://doi.org/10.1186/1471-2180-2-15 kostenfrei https://doaj.org/article/388cc46b0bcd4233ba3971e75e422198 kostenfrei http://www.biomedcentral.com/1471-2180/2/15 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1, p 15
allfields_unstemmed	10.1186/1471-2180-2-15 doi (DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198 DE-627 ger DE-627 rakwb eng QR1-502 Stephan Roger verfasserin aut Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p< Microbiology Corti Sabrina verfasserin aut In BMC Microbiology BMC, 2003 2(2002), 1, p 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:2 year:2002 number:1, p 15 https://doi.org/10.1186/1471-2180-2-15 kostenfrei https://doaj.org/article/388cc46b0bcd4233ba3971e75e422198 kostenfrei http://www.biomedcentral.com/1471-2180/2/15 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1, p 15
allfieldsGer	10.1186/1471-2180-2-15 doi (DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198 DE-627 ger DE-627 rakwb eng QR1-502 Stephan Roger verfasserin aut Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p< Microbiology Corti Sabrina verfasserin aut In BMC Microbiology BMC, 2003 2(2002), 1, p 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:2 year:2002 number:1, p 15 https://doi.org/10.1186/1471-2180-2-15 kostenfrei https://doaj.org/article/388cc46b0bcd4233ba3971e75e422198 kostenfrei http://www.biomedcentral.com/1471-2180/2/15 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1, p 15
allfieldsSound	10.1186/1471-2180-2-15 doi (DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198 DE-627 ger DE-627 rakwb eng QR1-502 Stephan Roger verfasserin aut Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p< Microbiology Corti Sabrina verfasserin aut In BMC Microbiology BMC, 2003 2(2002), 1, p 15 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:2 year:2002 number:1, p 15 https://doi.org/10.1186/1471-2180-2-15 kostenfrei https://doaj.org/article/388cc46b0bcd4233ba3971e75e422198 kostenfrei http://www.biomedcentral.com/1471-2180/2/15 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2002 1, p 15
language	English
source	In BMC Microbiology 2(2002), 1, p 15 volume:2 year:2002 number:1, p 15
sourceStr	In BMC Microbiology 2(2002), 1, p 15 volume:2 year:2002 number:1, p 15
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microbiology
isfreeaccess_bool	true
container_title	BMC Microbiology
authorswithroles_txt_mv	Stephan Roger @@aut@@ Corti Sabrina @@aut@@
publishDateDaySort_date	2002-01-01T00:00:00Z
hierarchy_top_id	326644997
id	DOAJ065086732
language_de	englisch
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Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. 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callnumber-first	Q - Science
author	Stephan Roger
spellingShingle	Stephan Roger misc QR1-502 misc Microbiology Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland
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topic_title	QR1-502 Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland
topic	misc QR1-502 misc Microbiology
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title	Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland
ctrlnum	(DE-627)DOAJ065086732 (DE-599)DOAJ388cc46b0bcd4233ba3971e75e422198
title_full	Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland
author_sort	Stephan Roger
journal	BMC Microbiology
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title_sort	detection of <it<mycobacterium avium subspecies paratuberculosis</it< specific is900 insertion sequences in bulk-tank milk samples obtained from different regions throughout switzerland
callnumber	QR1-502
title_auth	Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland
abstract	<p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Since <it<Mycobacterium avium</it< subspecies <it<paratuberculosis</it< (MAP) was isolated from intestinal tissue of a human patient suffering Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Raw milk may be a potential vehicle for the transmission of MAP to human population. In a previous paper, we have demonstrated that MAP are found in raw milk samples obtained from a defined region in Switzerland. The aim of this work is to collect data about the prevalence of MAP specific <it<IS900</it< insertion sequence in bulk-tank milk samples in different regions of Switzerland. Furthermore, we examined eventual correlation between the presence of MAP and the somatic cell counts, the total colony counts and the presence of <it<Enterobacteriaceae</it<.</p< <p<Results</p< <p<273 (19.7%) of the 1384 examined bulk-tank milk samples tested IS900 PCR-positive. The prevalence, however, in the different regions of Switzerland shows significant differences and ranged from 1.7% to 49.2%. Furthermore, there were no statistically significant (p << 0.05) differences between the somatic cell counts and the total colony counts of PCR-positive and PCR-negative milk samples. <it<Enterobacteriaceae</it< occur as often in IS900 PCR-positive as in PCR-negative milk samples.</p< <p<Conclusion</p< <p<This is the first study, which investigates the prevalence of MAP in bulk-tank milk samples all over Switzerland and infers the herd-level prevalence of MAP infection in dairy herds. The prevalence of 19.7% IS900 PCR-positive bulk-milk samples shows a wide distribution of subclinical MAP-infections in dairy stock in Switzerland. MAP can therefore often be transmitted to humans by raw milk consumption.</p<
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Detection of <it<Mycobacterium avium subspecies paratuberculosis</it< specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland

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