Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen
Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference i...
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Gespeichert in:
| Autor*in: |
Sasan Badi [verfasserIn] Hami Kaboosi [verfasserIn] Hossian Abbaspoor [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Persisch |
| Erschienen: |
2018 |
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| Übergeordnetes Werk: |
In: مجله دانشگاه علوم پزشکی گرگان - Golestan University of Medical Sciences, 2019, 20(2018), 3, Seite 70-75 |
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| Übergeordnetes Werk: |
volume:20 ; year:2018 ; number:3 ; pages:70-75 |
| Links: |
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DOAJ065164784 |
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| 520 | |a Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. | ||
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(DE-627)DOAJ065164784 (DE-599)DOAJ54b411dc224443d49e115ce164a40f41 DE-627 ger DE-627 rakwb per R5-920 Sasan Badi verfasserin aut Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. Digestive diseases Helicobacter pylori vacA gene alleles Medicine R Medicine (General) Hami Kaboosi verfasserin aut Hossian Abbaspoor verfasserin aut In مجله دانشگاه علوم پزشکی گرگان Golestan University of Medical Sciences, 2019 20(2018), 3, Seite 70-75 (DE-627)1760644765 20084080 nnns volume:20 year:2018 number:3 pages:70-75 https://doaj.org/article/54b411dc224443d49e115ce164a40f41 kostenfrei http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1562-4765 Journal toc kostenfrei https://doaj.org/toc/2008-4080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 20 2018 3 70-75 |
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(DE-627)DOAJ065164784 (DE-599)DOAJ54b411dc224443d49e115ce164a40f41 DE-627 ger DE-627 rakwb per R5-920 Sasan Badi verfasserin aut Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. Digestive diseases Helicobacter pylori vacA gene alleles Medicine R Medicine (General) Hami Kaboosi verfasserin aut Hossian Abbaspoor verfasserin aut In مجله دانشگاه علوم پزشکی گرگان Golestan University of Medical Sciences, 2019 20(2018), 3, Seite 70-75 (DE-627)1760644765 20084080 nnns volume:20 year:2018 number:3 pages:70-75 https://doaj.org/article/54b411dc224443d49e115ce164a40f41 kostenfrei http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1562-4765 Journal toc kostenfrei https://doaj.org/toc/2008-4080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 20 2018 3 70-75 |
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(DE-627)DOAJ065164784 (DE-599)DOAJ54b411dc224443d49e115ce164a40f41 DE-627 ger DE-627 rakwb per R5-920 Sasan Badi verfasserin aut Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. Digestive diseases Helicobacter pylori vacA gene alleles Medicine R Medicine (General) Hami Kaboosi verfasserin aut Hossian Abbaspoor verfasserin aut In مجله دانشگاه علوم پزشکی گرگان Golestan University of Medical Sciences, 2019 20(2018), 3, Seite 70-75 (DE-627)1760644765 20084080 nnns volume:20 year:2018 number:3 pages:70-75 https://doaj.org/article/54b411dc224443d49e115ce164a40f41 kostenfrei http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1562-4765 Journal toc kostenfrei https://doaj.org/toc/2008-4080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 20 2018 3 70-75 |
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(DE-627)DOAJ065164784 (DE-599)DOAJ54b411dc224443d49e115ce164a40f41 DE-627 ger DE-627 rakwb per R5-920 Sasan Badi verfasserin aut Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. Digestive diseases Helicobacter pylori vacA gene alleles Medicine R Medicine (General) Hami Kaboosi verfasserin aut Hossian Abbaspoor verfasserin aut In مجله دانشگاه علوم پزشکی گرگان Golestan University of Medical Sciences, 2019 20(2018), 3, Seite 70-75 (DE-627)1760644765 20084080 nnns volume:20 year:2018 number:3 pages:70-75 https://doaj.org/article/54b411dc224443d49e115ce164a40f41 kostenfrei http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1562-4765 Journal toc kostenfrei https://doaj.org/toc/2008-4080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 20 2018 3 70-75 |
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(DE-627)DOAJ065164784 (DE-599)DOAJ54b411dc224443d49e115ce164a40f41 DE-627 ger DE-627 rakwb per R5-920 Sasan Badi verfasserin aut Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. Digestive diseases Helicobacter pylori vacA gene alleles Medicine R Medicine (General) Hami Kaboosi verfasserin aut Hossian Abbaspoor verfasserin aut In مجله دانشگاه علوم پزشکی گرگان Golestan University of Medical Sciences, 2019 20(2018), 3, Seite 70-75 (DE-627)1760644765 20084080 nnns volume:20 year:2018 number:3 pages:70-75 https://doaj.org/article/54b411dc224443d49e115ce164a40f41 kostenfrei http://goums.ac.ir/journal/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1562-4765 Journal toc kostenfrei https://doaj.org/toc/2008-4080 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2055 GBV_ILN_2111 AR 20 2018 3 70-75 |
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The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. 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Prevalence of s1, s2, m1 and m2 alleles of vacA Helicobacter pylori gene isolated from clinical specimen |
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Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. |
| abstractGer |
Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. |
| abstract_unstemmed |
Background and Objective: Helicobacter pylori infection is a pathogenic agent of many stomach disorders, including peptic ulcer disease, stomach cancer and stomach lymphoma. The reasons for the variety of the outcomes of the infection resulting from Helicobacter pylori may be related to difference in genotype or expression of pathogenic bacterial-related factors, as well as environmental and host factors. This study was conducted to determine the frequency of s1, s2, m1 and m2 alleles of the vacA Helicobacter pylori gene isolated from clinical samples. Methods: This descriptive-analytic study was conducted on 183 patients whom suffering from digestive disorders which referring to the endoscopic department of Kordkuy’s Amiralmomenin hospital in Golestan province, north of Iran during 2016. Two samples of biopsy from antrum region were taken from each patient. The first sample was evaluated by urease test and the second one was done with saline buffer phosphate solution. Urease test of 50 positive samples and DNA extraction was performed. The polymerase chain reaction was performed for vacA alleles and then the relationship between toxin secretion with the symptoms such as abdominal pain, stomachache, reflux, nausea, gastritis, bleeding, stomach ulcers, burning, anemia, and weight loss were evaluated. Results: Frequency of s1, s2, m1, m2 vacA alleles of isolated strains was 88%, 6%, 38% and 70%, respectively. Also, the s1 / m1, s1 / m2, s2 / m1 and s2 / m2 genotypes of vacA Helicobacter pylori gene were determined 36%, 58%, 0% and 6%, respectively. Toxin secretion did not have significant relationship with digestive symptoms. Conclusion: The dominant genotype of the patients with digestive disorders (58%) in this study was s1 / m2 and s2 / m1 genotype did not observe in clinical samples. |
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