siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo

NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expre...
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Autor*in:	Peng Hu [verfasserIn] Xi Chen [verfasserIn] Jing Sun [verfasserIn] Ping Bie [verfasserIn] Lei‑Da Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	NUF2 pancreatic cancer RNA interference tumourigenesis treatment

Übergeordnetes Werk:	In: Bioscience Reports - Portland Press, Biochemical Society, 2018, 35(2015), 1, p e00170
Übergeordnetes Werk:	volume:35 ; year:2015 ; number:1, p e00170

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1042/BSR20140124

Katalog-ID:	DOAJ065213254

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spelling	10.1042/BSR20140124 doi (DE-627)DOAJ065213254 (DE-599)DOAJ31c7cd35b75d4ae59378b25922bee509 DE-627 ger DE-627 rakwb eng QH501-531 QR1-502 Peng Hu verfasserin aut siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer. NUF2 pancreatic cancer RNA interference tumourigenesis treatment Life Microbiology Xi Chen verfasserin aut Jing Sun verfasserin aut Ping Bie verfasserin aut Lei‑Da Zhang verfasserin aut In Bioscience Reports Portland Press, Biochemical Society, 2018 35(2015), 1, p e00170 (DE-627)320524485 (DE-600)2014993-1 15734935 nnns volume:35 year:2015 number:1, p e00170 https://doi.org/10.1042/BSR20140124 kostenfrei https://doaj.org/article/31c7cd35b75d4ae59378b25922bee509 kostenfrei http://www.bioscirep.org/bsr/035/e170/bsr035e170.htm kostenfrei https://doaj.org/toc/1573-4935 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2015 1, p e00170
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allfieldsSound	10.1042/BSR20140124 doi (DE-627)DOAJ065213254 (DE-599)DOAJ31c7cd35b75d4ae59378b25922bee509 DE-627 ger DE-627 rakwb eng QH501-531 QR1-502 Peng Hu verfasserin aut siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer. NUF2 pancreatic cancer RNA interference tumourigenesis treatment Life Microbiology Xi Chen verfasserin aut Jing Sun verfasserin aut Ping Bie verfasserin aut Lei‑Da Zhang verfasserin aut In Bioscience Reports Portland Press, Biochemical Society, 2018 35(2015), 1, p e00170 (DE-627)320524485 (DE-600)2014993-1 15734935 nnns volume:35 year:2015 number:1, p e00170 https://doi.org/10.1042/BSR20140124 kostenfrei https://doaj.org/article/31c7cd35b75d4ae59378b25922bee509 kostenfrei http://www.bioscirep.org/bsr/035/e170/bsr035e170.htm kostenfrei https://doaj.org/toc/1573-4935 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 35 2015 1, p e00170
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source	In Bioscience Reports 35(2015), 1, p e00170 volume:35 year:2015 number:1, p e00170
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topic_facet	NUF2 pancreatic cancer RNA interference tumourigenesis treatment Life Microbiology
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authorswithroles_txt_mv	Peng Hu @@aut@@ Xi Chen @@aut@@ Jing Sun @@aut@@ Ping Bie @@aut@@ Lei‑Da Zhang @@aut@@
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callnumber-first	Q - Science
author	Peng Hu
spellingShingle	Peng Hu misc QH501-531 misc QR1-502 misc NUF2 misc pancreatic cancer misc RNA interference misc tumourigenesis misc treatment misc Life misc Microbiology siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo
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topic_title	QH501-531 QR1-502 siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo NUF2 pancreatic cancer RNA interference tumourigenesis treatment
topic	misc QH501-531 misc QR1-502 misc NUF2 misc pancreatic cancer misc RNA interference misc tumourigenesis misc treatment misc Life misc Microbiology
topic_unstemmed	misc QH501-531 misc QR1-502 misc NUF2 misc pancreatic cancer misc RNA interference misc tumourigenesis misc treatment misc Life misc Microbiology
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title	siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo
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title_full	siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo
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title_sort	sirna-mediated knockdown against nuf2 suppresses pancreatic cancer proliferation in vitro and in vivo
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title_auth	siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo
abstract	NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer.
abstractGer	NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer.
abstract_unstemmed	NUF2 (NUF2, Ndc80 kinetochore complex component) plays an important role in kinetochore-microtubule attachment. It has been reported that NUF2 is associated with multiple human cancers. However, the functional role of NUF2 in pancreatic cancer remains unclear. In this study, we found that NUF2 expression was stronger in tumour tissues than in normal pancreatic tissues, and its overexpression could be related to poor prognosis. Moreover, NUF2 was highly expressed in several human pancreatic cancer cell lines. We took advantage of lentivirus-mediated siRNA (small interfering RNA) to suppress NUF2 expression in PANC-1 and Sw1990 cell lines aiming to investigate the role of NUF2 in pancreatic cancer. NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. Cell cycle analysis showed that NUF2 knockdown induced cell cycle arrest at G0/G1 phase via suppression of Cyclin B1, Cdc2 and Cdc25A. More importantly, NUF2 silencing was able to alleviate in vivo tumourigenesis in pancreatic cancer xenograft nude mice. Collectively, the present study indicates that the siRNA-mediated knockdown against NUF2 may be a promising therapeutic method for the treatment of pancreatic cancer.
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title_short	siRNA-mediated knockdown against NUF2 suppresses pancreatic cancer proliferation in vitro and in vivo
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