An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)

<p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx....
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Autor*in:	Garcia-Mas Jordi [verfasserIn] Aranda Miguel A [verfasserIn] Picó-Silvent Belén [verfasserIn] López-Bigas Nuria [verfasserIn] Deleu Wim [verfasserIn] Roig Cristina [verfasserIn] Saladié Montserrat [verfasserIn] Gonzalez-Ibeas Daniel [verfasserIn] Blanca José [verfasserIn] Mora-García Santiago [verfasserIn] Vilarrasa-Blasi Josep [verfasserIn] Cañizares Joaquin [verfasserIn] Mascarell-Creus Albert [verfasserIn] Nuez Fernando [verfasserIn] Puigdomènech Pere [verfasserIn] Caño-Delgado Ana I [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 10(2009), 1, p 467
Übergeordnetes Werk:	volume:10 ; year:2009 ; number:1, p 467

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-10-467

Katalog-ID:	DOAJ065267834

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allfields	10.1186/1471-2164-10-467 doi (DE-627)DOAJ065267834 (DE-599)DOAJ3f0177140e934feba0df8ff0d60beb5f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Garcia-Mas Jordi verfasserin aut An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.) 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p< Biotechnology Genetics Aranda Miguel A verfasserin aut Picó-Silvent Belén verfasserin aut López-Bigas Nuria verfasserin aut Deleu Wim verfasserin aut Roig Cristina verfasserin aut Saladié Montserrat verfasserin aut Gonzalez-Ibeas Daniel verfasserin aut Blanca José verfasserin aut Mora-García Santiago verfasserin aut Vilarrasa-Blasi Josep verfasserin aut Cañizares Joaquin verfasserin aut Mascarell-Creus Albert verfasserin aut Nuez Fernando verfasserin aut Puigdomènech Pere verfasserin aut Caño-Delgado Ana I verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 467 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 467 https://doi.org/10.1186/1471-2164-10-467 kostenfrei https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f kostenfrei http://www.biomedcentral.com/1471-2164/10/467 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 467
spelling	10.1186/1471-2164-10-467 doi (DE-627)DOAJ065267834 (DE-599)DOAJ3f0177140e934feba0df8ff0d60beb5f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Garcia-Mas Jordi verfasserin aut An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.) 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p< Biotechnology Genetics Aranda Miguel A verfasserin aut Picó-Silvent Belén verfasserin aut López-Bigas Nuria verfasserin aut Deleu Wim verfasserin aut Roig Cristina verfasserin aut Saladié Montserrat verfasserin aut Gonzalez-Ibeas Daniel verfasserin aut Blanca José verfasserin aut Mora-García Santiago verfasserin aut Vilarrasa-Blasi Josep verfasserin aut Cañizares Joaquin verfasserin aut Mascarell-Creus Albert verfasserin aut Nuez Fernando verfasserin aut Puigdomènech Pere verfasserin aut Caño-Delgado Ana I verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 467 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 467 https://doi.org/10.1186/1471-2164-10-467 kostenfrei https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f kostenfrei http://www.biomedcentral.com/1471-2164/10/467 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 467
allfields_unstemmed	10.1186/1471-2164-10-467 doi (DE-627)DOAJ065267834 (DE-599)DOAJ3f0177140e934feba0df8ff0d60beb5f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Garcia-Mas Jordi verfasserin aut An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.) 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p< Biotechnology Genetics Aranda Miguel A verfasserin aut Picó-Silvent Belén verfasserin aut López-Bigas Nuria verfasserin aut Deleu Wim verfasserin aut Roig Cristina verfasserin aut Saladié Montserrat verfasserin aut Gonzalez-Ibeas Daniel verfasserin aut Blanca José verfasserin aut Mora-García Santiago verfasserin aut Vilarrasa-Blasi Josep verfasserin aut Cañizares Joaquin verfasserin aut Mascarell-Creus Albert verfasserin aut Nuez Fernando verfasserin aut Puigdomènech Pere verfasserin aut Caño-Delgado Ana I verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 467 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 467 https://doi.org/10.1186/1471-2164-10-467 kostenfrei https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f kostenfrei http://www.biomedcentral.com/1471-2164/10/467 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 467
allfieldsGer	10.1186/1471-2164-10-467 doi (DE-627)DOAJ065267834 (DE-599)DOAJ3f0177140e934feba0df8ff0d60beb5f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Garcia-Mas Jordi verfasserin aut An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.) 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p< Biotechnology Genetics Aranda Miguel A verfasserin aut Picó-Silvent Belén verfasserin aut López-Bigas Nuria verfasserin aut Deleu Wim verfasserin aut Roig Cristina verfasserin aut Saladié Montserrat verfasserin aut Gonzalez-Ibeas Daniel verfasserin aut Blanca José verfasserin aut Mora-García Santiago verfasserin aut Vilarrasa-Blasi Josep verfasserin aut Cañizares Joaquin verfasserin aut Mascarell-Creus Albert verfasserin aut Nuez Fernando verfasserin aut Puigdomènech Pere verfasserin aut Caño-Delgado Ana I verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 467 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 467 https://doi.org/10.1186/1471-2164-10-467 kostenfrei https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f kostenfrei http://www.biomedcentral.com/1471-2164/10/467 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 467
allfieldsSound	10.1186/1471-2164-10-467 doi (DE-627)DOAJ065267834 (DE-599)DOAJ3f0177140e934feba0df8ff0d60beb5f DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Garcia-Mas Jordi verfasserin aut An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.) 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p< Biotechnology Genetics Aranda Miguel A verfasserin aut Picó-Silvent Belén verfasserin aut López-Bigas Nuria verfasserin aut Deleu Wim verfasserin aut Roig Cristina verfasserin aut Saladié Montserrat verfasserin aut Gonzalez-Ibeas Daniel verfasserin aut Blanca José verfasserin aut Mora-García Santiago verfasserin aut Vilarrasa-Blasi Josep verfasserin aut Cañizares Joaquin verfasserin aut Mascarell-Creus Albert verfasserin aut Nuez Fernando verfasserin aut Puigdomènech Pere verfasserin aut Caño-Delgado Ana I verfasserin aut In BMC Genomics BMC, 2003 10(2009), 1, p 467 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:10 year:2009 number:1, p 467 https://doi.org/10.1186/1471-2164-10-467 kostenfrei https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f kostenfrei http://www.biomedcentral.com/1471-2164/10/467 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2009 1, p 467
language	English
source	In BMC Genomics 10(2009), 1, p 467 volume:10 year:2009 number:1, p 467
sourceStr	In BMC Genomics 10(2009), 1, p 467 volume:10 year:2009 number:1, p 467
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Garcia-Mas Jordi @@aut@@ Aranda Miguel A @@aut@@ Picó-Silvent Belén @@aut@@ López-Bigas Nuria @@aut@@ Deleu Wim @@aut@@ Roig Cristina @@aut@@ Saladié Montserrat @@aut@@ Gonzalez-Ibeas Daniel @@aut@@ Blanca José @@aut@@ Mora-García Santiago @@aut@@ Vilarrasa-Blasi Josep @@aut@@ Cañizares Joaquin @@aut@@ Mascarell-Creus Albert @@aut@@ Nuez Fernando @@aut@@ Puigdomènech Pere @@aut@@ Caño-Delgado Ana I @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ065267834
language_de	englisch
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Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. 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callnumber-first	T - Technology
author	Garcia-Mas Jordi
spellingShingle	Garcia-Mas Jordi misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
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topic_title	TP248.13-248.65 QH426-470 An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
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title	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
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title_full	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
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author_browse	Garcia-Mas Jordi Aranda Miguel A Picó-Silvent Belén López-Bigas Nuria Deleu Wim Roig Cristina Saladié Montserrat Gonzalez-Ibeas Daniel Blanca José Mora-García Santiago Vilarrasa-Blasi Josep Cañizares Joaquin Mascarell-Creus Albert Nuez Fernando Puigdomènech Pere Caño-Delgado Ana I
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title_sort	oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<cucumis melo </it<l.)
callnumber	TP248.13-248.65
title_auth	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
abstract	<p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Melon (<it<Cucumis melo</it<) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.</p< <p<Results</p< <p<We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.</p< <p<Conclusion</p< <p<The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.</p<
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title_short	An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)
url	https://doi.org/10.1186/1471-2164-10-467 https://doaj.org/article/3f0177140e934feba0df8ff0d60beb5f http://www.biomedcentral.com/1471-2164/10/467 https://doaj.org/toc/1471-2164
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An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (<it<Cucumis melo </it<L.)

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