<i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish

The anticonvulsive potential of proteins extracted from <i<Orthosiphon stamineus</i< leaves (OSLP) has never been elucidated in zebrafish (<i<Danio rerio</i<). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure mo...
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Gespeichert in:

Autor*in:	Yin-Sir Chung [verfasserIn] Brandon Kar Meng Choo [verfasserIn] Pervaiz Khalid Ahmed [verfasserIn] Iekhsan Othman [verfasserIn] Mohd. Farooq Shaikh [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	plant-derived proteins epilepsy seizures zebrafish

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 8(2020), 7, p 191
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:7, p 191

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines8070191

Katalog-ID:	DOAJ06538203X

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allfieldsSound	10.3390/biomedicines8070191 doi (DE-627)DOAJ06538203X (DE-599)DOAJ0c22607e74de4a8b8237cafdb650d033 DE-627 ger DE-627 rakwb eng QH301-705.5 Yin-Sir Chung verfasserin aut <i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The anticonvulsive potential of proteins extracted from <i<Orthosiphon stamineus</i< leaves (OSLP) has never been elucidated in zebrafish (<i<Danio rerio</i<). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP. <i<Orthosiphon stamineus</i< plant-derived proteins epilepsy seizures zebrafish Biology (General) Brandon Kar Meng Choo verfasserin aut Pervaiz Khalid Ahmed verfasserin aut Iekhsan Othman verfasserin aut Mohd. Farooq Shaikh verfasserin aut In Biomedicines MDPI AG, 2014 8(2020), 7, p 191 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:8 year:2020 number:7, p 191 https://doi.org/10.3390/biomedicines8070191 kostenfrei https://doaj.org/article/0c22607e74de4a8b8237cafdb650d033 kostenfrei https://www.mdpi.com/2227-9059/8/7/191 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 7, p 191
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callnumber-first	Q - Science
author	Yin-Sir Chung
spellingShingle	Yin-Sir Chung misc QH301-705.5 misc <i<Orthosiphon stamineus</i< misc plant-derived proteins misc epilepsy misc seizures misc zebrafish misc Biology (General) <i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
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topic_title	QH301-705.5 <i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish <i<Orthosiphon stamineus</i< plant-derived proteins epilepsy seizures zebrafish
topic	misc QH301-705.5 misc <i<Orthosiphon stamineus</i< misc plant-derived proteins misc epilepsy misc seizures misc zebrafish misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Orthosiphon stamineus</i< misc plant-derived proteins misc epilepsy misc seizures misc zebrafish misc Biology (General)
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title	<i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
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title_full	<i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
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author_browse	Yin-Sir Chung Brandon Kar Meng Choo Pervaiz Khalid Ahmed Iekhsan Othman Mohd. Farooq Shaikh
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title_sort	<i<orthosiphon stamineus</i< proteins alleviate pentylenetetrazol-induced seizures in zebrafish
callnumber	QH301-705.5
title_auth	<i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
abstract	The anticonvulsive potential of proteins extracted from <i<Orthosiphon stamineus</i< leaves (OSLP) has never been elucidated in zebrafish (<i<Danio rerio</i<). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP.
abstractGer	The anticonvulsive potential of proteins extracted from <i<Orthosiphon stamineus</i< leaves (OSLP) has never been elucidated in zebrafish (<i<Danio rerio</i<). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP.
abstract_unstemmed	The anticonvulsive potential of proteins extracted from <i<Orthosiphon stamineus</i< leaves (OSLP) has never been elucidated in zebrafish (<i<Danio rerio</i<). This study thus aims to elucidate the anticonvulsive potential of OSLP in pentylenetetrazol (PTZ)-induced seizure model. Physical changes (seizure score and seizure onset time, behavior, locomotor) and neurotransmitter analysis were elucidated to assess the pharmacological activity. The protective mechanism of OSLP on brain was also studied using mass spectrometry-based label-free proteomic quantification (LFQ) and bioinformatics. OSLP was found to be safe up to 800 µg/kg and pre-treatment with OSLP (800 µg/kg, i.p., 30 min) decreased the frequency of convulsive activities (lower seizure score and prolonged seizure onset time), improved locomotor behaviors (reduced erratic swimming movements and bottom-dwelling habit), and lowered the excitatory neurotransmitter (glutamate). Pre-treatment with OSLP increased protein Complexin 2 (Cplx 2) expression in the zebrafish brain. Cplx2 is an important regulator in the trans-SNARE complex which is required during the vesicle priming phase in the calcium-dependent synaptic vesicle exocytosis. Findings in this study collectively suggests that OSLP could be regulating the release of neurotransmitters via calcium-dependent synaptic vesicle exocytosis mediated by the “Synaptic Vesicle Cycle” pathway. OSLP’s anticonvulsive actions could be acting differently from diazepam (DZP) and with that, it might not produce the similar cognitive insults such as DZP.
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container_issue	7, p 191
title_short	<i<Orthosiphon stamineus</i< Proteins Alleviate Pentylenetetrazol-Induced Seizures in Zebrafish
url	https://doi.org/10.3390/biomedicines8070191 https://doaj.org/article/0c22607e74de4a8b8237cafdb650d033 https://www.mdpi.com/2227-9059/8/7/191 https://doaj.org/toc/2227-9059
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author2	Brandon Kar Meng Choo Pervaiz Khalid Ahmed Iekhsan Othman Mohd. Farooq Shaikh
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up_date	2024-07-03T14:27:54.343Z
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