Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permea...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Diliana Pérez-Reytor [verfasserIn] Alequis Pavón [verfasserIn] Carmen Lopez-Joven [verfasserIn] Sebastián Ramírez-Araya [verfasserIn] Carlos Peña-Varas [verfasserIn] Nicolás Plaza [verfasserIn] Melissa Alegría-Arcos [verfasserIn] Gino Corsini [verfasserIn] Víctor Jaña [verfasserIn] Leonardo Pavez [verfasserIn] Talia del Pozo [verfasserIn] Roberto Bastías [verfasserIn] Carlos J. Blondel [verfasserIn] David Ramírez [verfasserIn] Katherine García [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Frontiers in Cellular and Infection Microbiology - Frontiers Media S.A., 2016, 10(2020) |
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| Übergeordnetes Werk: |
volume:10 ; year:2020 |
| Links: |
|---|
| DOI / URN: |
10.3389/fcimb.2020.00482 |
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| Katalog-ID: |
DOAJ065392930 |
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| 520 | |a Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. | ||
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| 650 | 4 | |a Zonula occludens toxin | |
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10.3389/fcimb.2020.00482 doi (DE-627)DOAJ065392930 (DE-599)DOAJ633f5fd49ade4c2b8617c616d3814273 DE-627 ger DE-627 rakwb eng QR1-502 Diliana Pérez-Reytor verfasserin aut Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. Vibrio parahaemolyticus non-toxigenic strains Zonula occludens toxin Zot Vibrio cholerae Campylobacter concisus Microbiology Alequis Pavón verfasserin aut Carmen Lopez-Joven verfasserin aut Sebastián Ramírez-Araya verfasserin aut Carlos Peña-Varas verfasserin aut Nicolás Plaza verfasserin aut Melissa Alegría-Arcos verfasserin aut Gino Corsini verfasserin aut Víctor Jaña verfasserin aut Leonardo Pavez verfasserin aut Leonardo Pavez verfasserin aut Talia del Pozo verfasserin aut Roberto Bastías verfasserin aut Carlos J. Blondel verfasserin aut Carlos J. Blondel verfasserin aut David Ramírez verfasserin aut Katherine García verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 10(2020) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:10 year:2020 https://doi.org/10.3389/fcimb.2020.00482 kostenfrei https://doaj.org/article/633f5fd49ade4c2b8617c616d3814273 kostenfrei https://www.frontiersin.org/article/10.3389/fcimb.2020.00482/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 |
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10.3389/fcimb.2020.00482 doi (DE-627)DOAJ065392930 (DE-599)DOAJ633f5fd49ade4c2b8617c616d3814273 DE-627 ger DE-627 rakwb eng QR1-502 Diliana Pérez-Reytor verfasserin aut Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. Vibrio parahaemolyticus non-toxigenic strains Zonula occludens toxin Zot Vibrio cholerae Campylobacter concisus Microbiology Alequis Pavón verfasserin aut Carmen Lopez-Joven verfasserin aut Sebastián Ramírez-Araya verfasserin aut Carlos Peña-Varas verfasserin aut Nicolás Plaza verfasserin aut Melissa Alegría-Arcos verfasserin aut Gino Corsini verfasserin aut Víctor Jaña verfasserin aut Leonardo Pavez verfasserin aut Leonardo Pavez verfasserin aut Talia del Pozo verfasserin aut Roberto Bastías verfasserin aut Carlos J. Blondel verfasserin aut Carlos J. Blondel verfasserin aut David Ramírez verfasserin aut Katherine García verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 10(2020) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:10 year:2020 https://doi.org/10.3389/fcimb.2020.00482 kostenfrei https://doaj.org/article/633f5fd49ade4c2b8617c616d3814273 kostenfrei https://www.frontiersin.org/article/10.3389/fcimb.2020.00482/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 |
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10.3389/fcimb.2020.00482 doi (DE-627)DOAJ065392930 (DE-599)DOAJ633f5fd49ade4c2b8617c616d3814273 DE-627 ger DE-627 rakwb eng QR1-502 Diliana Pérez-Reytor verfasserin aut Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. Vibrio parahaemolyticus non-toxigenic strains Zonula occludens toxin Zot Vibrio cholerae Campylobacter concisus Microbiology Alequis Pavón verfasserin aut Carmen Lopez-Joven verfasserin aut Sebastián Ramírez-Araya verfasserin aut Carlos Peña-Varas verfasserin aut Nicolás Plaza verfasserin aut Melissa Alegría-Arcos verfasserin aut Gino Corsini verfasserin aut Víctor Jaña verfasserin aut Leonardo Pavez verfasserin aut Leonardo Pavez verfasserin aut Talia del Pozo verfasserin aut Roberto Bastías verfasserin aut Carlos J. Blondel verfasserin aut Carlos J. Blondel verfasserin aut David Ramírez verfasserin aut Katherine García verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 10(2020) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:10 year:2020 https://doi.org/10.3389/fcimb.2020.00482 kostenfrei https://doaj.org/article/633f5fd49ade4c2b8617c616d3814273 kostenfrei https://www.frontiersin.org/article/10.3389/fcimb.2020.00482/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 |
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10.3389/fcimb.2020.00482 doi (DE-627)DOAJ065392930 (DE-599)DOAJ633f5fd49ade4c2b8617c616d3814273 DE-627 ger DE-627 rakwb eng QR1-502 Diliana Pérez-Reytor verfasserin aut Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. Vibrio parahaemolyticus non-toxigenic strains Zonula occludens toxin Zot Vibrio cholerae Campylobacter concisus Microbiology Alequis Pavón verfasserin aut Carmen Lopez-Joven verfasserin aut Sebastián Ramírez-Araya verfasserin aut Carlos Peña-Varas verfasserin aut Nicolás Plaza verfasserin aut Melissa Alegría-Arcos verfasserin aut Gino Corsini verfasserin aut Víctor Jaña verfasserin aut Leonardo Pavez verfasserin aut Leonardo Pavez verfasserin aut Talia del Pozo verfasserin aut Roberto Bastías verfasserin aut Carlos J. Blondel verfasserin aut Carlos J. Blondel verfasserin aut David Ramírez verfasserin aut Katherine García verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 10(2020) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:10 year:2020 https://doi.org/10.3389/fcimb.2020.00482 kostenfrei https://doaj.org/article/633f5fd49ade4c2b8617c616d3814273 kostenfrei https://www.frontiersin.org/article/10.3389/fcimb.2020.00482/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2020 |
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Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 |
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Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 |
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Diliana Pérez-Reytor |
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Frontiers in Cellular and Infection Microbiology |
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Diliana Pérez-Reytor Alequis Pavón Carmen Lopez-Joven Sebastián Ramírez-Araya Carlos Peña-Varas Nicolás Plaza Melissa Alegría-Arcos Gino Corsini Víctor Jaña Leonardo Pavez Talia del Pozo Roberto Bastías Carlos J. Blondel David Ramírez Katherine García |
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analysis of the zonula occludens toxin found in the genome of the chilean non-toxigenic vibrio parahaemolyticus strain pmc53.7 |
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Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 |
| abstract |
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. |
| abstractGer |
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. |
| abstract_unstemmed |
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V. parahaemolyticus strains, including the non-toxigenic clinical strain PMC53.7. To gain insights into a possible role of Zot in V. parahaemolyticus, we analyzed whether it could be responsible for cytotoxicity. However, we observed a barely positive correlation between Caco-2 cell membrane damage and Zot mRNA expression during PMC53.7 infection and non-cytotoxicity induction in response to purified PMC53.7-Zot. Unusually, we observed a particular actin disturbance on cells infected with PMC53.7. Based on this observation, we decided to compare the sequence of PMC53.7-Zot with Zot of human pathogenic species such as V. cholerae, Campylobacter concisus, Neisseria meningitidis, and other V. parahaemolyticus strains, using computational tools. The PMC53.7-Zot was compared with other toxins and identified as an endotoxin with conserved motifs in the N-terminus and a variable C-terminal region and without FCIGRL peptide. Notably, the C-terminal diversity among Zots meant that not all of them could be identified as toxins. Structurally, PMC53.7-Zot was modeled as a transmembrane protein. Our results suggested that it has partial 3D structure similarity with V. cholerae-Zot. Probably, the PMC53.7-Zot would affect the actin cytoskeletal, but, in the absence of FCIGRL, the mechanisms of actions must be elucidated. |
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Analysis of the Zonula occludens Toxin Found in the Genome of the Chilean Non-toxigenic Vibrio parahaemolyticus Strain PMC53.7 |
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Alequis Pavón Carmen Lopez-Joven Sebastián Ramírez-Araya Carlos Peña-Varas Nicolás Plaza Melissa Alegría-Arcos Gino Corsini Víctor Jaña Leonardo Pavez Talia del Pozo Roberto Bastías Carlos J. Blondel David Ramírez Katherine García |
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Alequis Pavón Carmen Lopez-Joven Sebastián Ramírez-Araya Carlos Peña-Varas Nicolás Plaza Melissa Alegría-Arcos Gino Corsini Víctor Jaña Leonardo Pavez Talia del Pozo Roberto Bastías Carlos J. Blondel David Ramírez Katherine García |
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