Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules

BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual li...
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Autor*in:	Hyemi Kwon [verfasserIn] Won Gu Kim [verfasserIn] Markus Eszlinger [verfasserIn] Ralf Paschke [verfasserIn] Dong Eun Song [verfasserIn] Mijin Kim [verfasserIn] Suyeon Park [verfasserIn] Min Ji Jeon [verfasserIn] Tae Yong Kim [verfasserIn] Young Kee Shong [verfasserIn] Won Bae Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Koreanisch

Erschienen:	2016

Schlagwörter:	Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms

Übergeordnetes Werk:	In: Endocrinology and Metabolism - Korean Endocrine Society, 2016, 31(2016), 4, Seite 586-591
Übergeordnetes Werk:	volume:31 ; year:2016 ; number:4 ; pages:586-591

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3803/EnM.2016.31.4.586

Katalog-ID:	DOAJ065433866

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520			\|a BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
650		4	\|a Molecular diagnostic techniques
650		4	\|a Biopsy, fine-needle
650		4	\|a Thyroid aspiration
650		4	\|a Thyroid nodule
650		4	\|a Thyroid neoplasms
653		0	\|a Diseases of the endocrine glands. Clinical endocrinology
700	0		\|a Won Gu Kim \|e verfasserin \|4 aut
700	0		\|a Markus Eszlinger \|e verfasserin \|4 aut
700	0		\|a Ralf Paschke \|e verfasserin \|4 aut
700	0		\|a Dong Eun Song \|e verfasserin \|4 aut
700	0		\|a Mijin Kim \|e verfasserin \|4 aut
700	0		\|a Suyeon Park \|e verfasserin \|4 aut
700	0		\|a Min Ji Jeon \|e verfasserin \|4 aut
700	0		\|a Tae Yong Kim \|e verfasserin \|4 aut
700	0		\|a Young Kee Shong \|e verfasserin \|4 aut
700	0		\|a Won Bae Kim \|e verfasserin \|4 aut
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author_variant	h k hk w g k wgk m e me r p rp d e s des m k mk s p sp m j j mjj t y k tyk y k s yks w b k wbk
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allfields	10.3803/EnM.2016.31.4.586 doi (DE-627)DOAJ065433866 (DE-599)DOAJb04f4a8b11cf404d8ee84271d38fa237 DE-627 ger DE-627 rakwb eng kor RC648-665 Hyemi Kwon verfasserin aut Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules. Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology Won Gu Kim verfasserin aut Markus Eszlinger verfasserin aut Ralf Paschke verfasserin aut Dong Eun Song verfasserin aut Mijin Kim verfasserin aut Suyeon Park verfasserin aut Min Ji Jeon verfasserin aut Tae Yong Kim verfasserin aut Young Kee Shong verfasserin aut Won Bae Kim verfasserin aut In Endocrinology and Metabolism Korean Endocrine Society, 2016 31(2016), 4, Seite 586-591 (DE-627)810542803 (DE-600)2802452-7 20935978 nnns volume:31 year:2016 number:4 pages:586-591 https://doi.org/10.3803/EnM.2016.31.4.586 kostenfrei https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 kostenfrei http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf kostenfrei https://doaj.org/toc/2093-596X Journal toc kostenfrei https://doaj.org/toc/2093-5978 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2016 4 586-591
spelling	10.3803/EnM.2016.31.4.586 doi (DE-627)DOAJ065433866 (DE-599)DOAJb04f4a8b11cf404d8ee84271d38fa237 DE-627 ger DE-627 rakwb eng kor RC648-665 Hyemi Kwon verfasserin aut Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules. Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology Won Gu Kim verfasserin aut Markus Eszlinger verfasserin aut Ralf Paschke verfasserin aut Dong Eun Song verfasserin aut Mijin Kim verfasserin aut Suyeon Park verfasserin aut Min Ji Jeon verfasserin aut Tae Yong Kim verfasserin aut Young Kee Shong verfasserin aut Won Bae Kim verfasserin aut In Endocrinology and Metabolism Korean Endocrine Society, 2016 31(2016), 4, Seite 586-591 (DE-627)810542803 (DE-600)2802452-7 20935978 nnns volume:31 year:2016 number:4 pages:586-591 https://doi.org/10.3803/EnM.2016.31.4.586 kostenfrei https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 kostenfrei http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf kostenfrei https://doaj.org/toc/2093-596X Journal toc kostenfrei https://doaj.org/toc/2093-5978 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2016 4 586-591
allfields_unstemmed	10.3803/EnM.2016.31.4.586 doi (DE-627)DOAJ065433866 (DE-599)DOAJb04f4a8b11cf404d8ee84271d38fa237 DE-627 ger DE-627 rakwb eng kor RC648-665 Hyemi Kwon verfasserin aut Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules. Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology Won Gu Kim verfasserin aut Markus Eszlinger verfasserin aut Ralf Paschke verfasserin aut Dong Eun Song verfasserin aut Mijin Kim verfasserin aut Suyeon Park verfasserin aut Min Ji Jeon verfasserin aut Tae Yong Kim verfasserin aut Young Kee Shong verfasserin aut Won Bae Kim verfasserin aut In Endocrinology and Metabolism Korean Endocrine Society, 2016 31(2016), 4, Seite 586-591 (DE-627)810542803 (DE-600)2802452-7 20935978 nnns volume:31 year:2016 number:4 pages:586-591 https://doi.org/10.3803/EnM.2016.31.4.586 kostenfrei https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 kostenfrei http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf kostenfrei https://doaj.org/toc/2093-596X Journal toc kostenfrei https://doaj.org/toc/2093-5978 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2016 4 586-591
allfieldsGer	10.3803/EnM.2016.31.4.586 doi (DE-627)DOAJ065433866 (DE-599)DOAJb04f4a8b11cf404d8ee84271d38fa237 DE-627 ger DE-627 rakwb eng kor RC648-665 Hyemi Kwon verfasserin aut Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules. Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology Won Gu Kim verfasserin aut Markus Eszlinger verfasserin aut Ralf Paschke verfasserin aut Dong Eun Song verfasserin aut Mijin Kim verfasserin aut Suyeon Park verfasserin aut Min Ji Jeon verfasserin aut Tae Yong Kim verfasserin aut Young Kee Shong verfasserin aut Won Bae Kim verfasserin aut In Endocrinology and Metabolism Korean Endocrine Society, 2016 31(2016), 4, Seite 586-591 (DE-627)810542803 (DE-600)2802452-7 20935978 nnns volume:31 year:2016 number:4 pages:586-591 https://doi.org/10.3803/EnM.2016.31.4.586 kostenfrei https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 kostenfrei http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf kostenfrei https://doaj.org/toc/2093-596X Journal toc kostenfrei https://doaj.org/toc/2093-5978 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2016 4 586-591
allfieldsSound	10.3803/EnM.2016.31.4.586 doi (DE-627)DOAJ065433866 (DE-599)DOAJb04f4a8b11cf404d8ee84271d38fa237 DE-627 ger DE-627 rakwb eng kor RC648-665 Hyemi Kwon verfasserin aut Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules. Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology Won Gu Kim verfasserin aut Markus Eszlinger verfasserin aut Ralf Paschke verfasserin aut Dong Eun Song verfasserin aut Mijin Kim verfasserin aut Suyeon Park verfasserin aut Min Ji Jeon verfasserin aut Tae Yong Kim verfasserin aut Young Kee Shong verfasserin aut Won Bae Kim verfasserin aut In Endocrinology and Metabolism Korean Endocrine Society, 2016 31(2016), 4, Seite 586-591 (DE-627)810542803 (DE-600)2802452-7 20935978 nnns volume:31 year:2016 number:4 pages:586-591 https://doi.org/10.3803/EnM.2016.31.4.586 kostenfrei https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 kostenfrei http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf kostenfrei https://doaj.org/toc/2093-596X Journal toc kostenfrei https://doaj.org/toc/2093-5978 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 31 2016 4 586-591
language	English Korean
source	In Endocrinology and Metabolism 31(2016), 4, Seite 586-591 volume:31 year:2016 number:4 pages:586-591
sourceStr	In Endocrinology and Metabolism 31(2016), 4, Seite 586-591 volume:31 year:2016 number:4 pages:586-591
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms Diseases of the endocrine glands. Clinical endocrinology
isfreeaccess_bool	true
container_title	Endocrinology and Metabolism
authorswithroles_txt_mv	Hyemi Kwon @@aut@@ Won Gu Kim @@aut@@ Markus Eszlinger @@aut@@ Ralf Paschke @@aut@@ Dong Eun Song @@aut@@ Mijin Kim @@aut@@ Suyeon Park @@aut@@ Min Ji Jeon @@aut@@ Tae Yong Kim @@aut@@ Young Kee Shong @@aut@@ Won Bae Kim @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	810542803
id	DOAJ065433866
language_de	englisch koreanisch
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callnumber-first	R - Medicine
author	Hyemi Kwon
spellingShingle	Hyemi Kwon misc RC648-665 misc Molecular diagnostic techniques misc Biopsy, fine-needle misc Thyroid aspiration misc Thyroid nodule misc Thyroid neoplasms misc Diseases of the endocrine glands. Clinical endocrinology Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
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topic_title	RC648-665 Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules Molecular diagnostic techniques Biopsy, fine-needle Thyroid aspiration Thyroid nodule Thyroid neoplasms
topic	misc RC648-665 misc Molecular diagnostic techniques misc Biopsy, fine-needle misc Thyroid aspiration misc Thyroid nodule misc Thyroid neoplasms misc Diseases of the endocrine glands. Clinical endocrinology
topic_unstemmed	misc RC648-665 misc Molecular diagnostic techniques misc Biopsy, fine-needle misc Thyroid aspiration misc Thyroid nodule misc Thyroid neoplasms misc Diseases of the endocrine glands. Clinical endocrinology
topic_browse	misc RC648-665 misc Molecular diagnostic techniques misc Biopsy, fine-needle misc Thyroid aspiration misc Thyroid nodule misc Thyroid neoplasms misc Diseases of the endocrine glands. Clinical endocrinology
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title	Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
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title_full	Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
author_sort	Hyemi Kwon
journal	Endocrinology and Metabolism
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author_browse	Hyemi Kwon Won Gu Kim Markus Eszlinger Ralf Paschke Dong Eun Song Mijin Kim Suyeon Park Min Ji Jeon Tae Yong Kim Young Kee Shong Won Bae Kim
container_volume	31
class	RC648-665
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author-letter	Hyemi Kwon
doi_str_mv	10.3803/EnM.2016.31.4.586
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title_sort	molecular diagnosis using residual liquid-based cytology materials for patients with nondiagnostic or indeterminate thyroid nodules
callnumber	RC648-665
title_auth	Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
abstract	BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
abstractGer	BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
abstract_unstemmed	BackgroundMolecular analysis for common somatic mutations in thyroid cancer can improve diagnostic accuracy of fine-needle aspiration cytology (FNAC) in the nondiagnostic or indeterminate category of thyroid nodules. In this study, we evaluated the feasibility of molecular diagnosis from residual liquid-based cytology (LBC) material after cytological diagnosis.MethodsThis prospective study enrolled 53 patients with thyroid nodules diagnosed as nondiagnostic, atypia of undetermined significance (AUS), or follicular lesion of undetermined significance (FLUS) after FNAC. DNAs and RNAs were isolated from residual LBC materials. BRAFV600E and RAS point mutations, PAX8/peroxisome proliferator-activated receptor γ (PPARγ), RET/PTC1, and RET/PTC3 rearrangements were evaluated by real-time polymerase chain reaction and pyrosequencing.ResultsAll DNAs from 53 residual LBC samples could be analysed and point mutations were detected in 10 samples (19%). In 17 AUS nodules, seven samples (41%) had point mutations including BRAF (n=4), NRAS (n=2), and KRAS (n=1). In 20 FLUS nodules, three samples (15%) had NRAS point mutations. RNA from only one FLUS nodule could be analysed for rearrangements and there was no abnormality.ConclusionMolecular analysis for BRAF and RAS mutations was feasible in residual LBC materials and might be useful for diagnosis of indeterminate thyroid nodules.
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container_issue	4
title_short	Molecular Diagnosis Using Residual Liquid-Based Cytology Materials for Patients with Nondiagnostic or Indeterminate Thyroid Nodules
url	https://doi.org/10.3803/EnM.2016.31.4.586 https://doaj.org/article/b04f4a8b11cf404d8ee84271d38fa237 http://e-enm.org/Synapse/Data/PDFData/2008ENM/enm-31-586.pdf https://doaj.org/toc/2093-596X https://doaj.org/toc/2093-5978
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author2	Won Gu Kim Markus Eszlinger Ralf Paschke Dong Eun Song Mijin Kim Suyeon Park Min Ji Jeon Tae Yong Kim Young Kee Shong Won Bae Kim
author2Str	Won Gu Kim Markus Eszlinger Ralf Paschke Dong Eun Song Mijin Kim Suyeon Park Min Ji Jeon Tae Yong Kim Young Kee Shong Won Bae Kim
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up_date	2024-07-03T14:44:44.316Z
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