Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene
Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to...
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Shahzad Rahmandoost [verfasserIn] Kumarss Amini [verfasserIn] |
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2015 |
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In: مجله دانشکده پزشکی اصفهان - Isfahan University of Medical Sciences, 2018, 33(2015), 356, Seite 1804-1809 |
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volume:33 ; year:2015 ; number:356 ; pages:1804-1809 |
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(DE-627)DOAJ06590303X (DE-599)DOAJfac000a8242741779cad9ebbde7405af DE-627 ger DE-627 rakwb per R5-920 Shahzad Rahmandoost verfasserin aut Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. Streptococcus mutans Glucosyltransferase gtfB gene Medicine R Medicine (General) Kumarss Amini verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 33(2015), 356, Seite 1804-1809 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:33 year:2015 number:356 pages:1804-1809 https://doaj.org/article/fac000a8242741779cad9ebbde7405af kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/4826 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 33 2015 356 1804-1809 |
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(DE-627)DOAJ06590303X (DE-599)DOAJfac000a8242741779cad9ebbde7405af DE-627 ger DE-627 rakwb per R5-920 Shahzad Rahmandoost verfasserin aut Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. Streptococcus mutans Glucosyltransferase gtfB gene Medicine R Medicine (General) Kumarss Amini verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 33(2015), 356, Seite 1804-1809 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:33 year:2015 number:356 pages:1804-1809 https://doaj.org/article/fac000a8242741779cad9ebbde7405af kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/4826 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 33 2015 356 1804-1809 |
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(DE-627)DOAJ06590303X (DE-599)DOAJfac000a8242741779cad9ebbde7405af DE-627 ger DE-627 rakwb per R5-920 Shahzad Rahmandoost verfasserin aut Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. Streptococcus mutans Glucosyltransferase gtfB gene Medicine R Medicine (General) Kumarss Amini verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 33(2015), 356, Seite 1804-1809 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:33 year:2015 number:356 pages:1804-1809 https://doaj.org/article/fac000a8242741779cad9ebbde7405af kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/4826 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 33 2015 356 1804-1809 |
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(DE-627)DOAJ06590303X (DE-599)DOAJfac000a8242741779cad9ebbde7405af DE-627 ger DE-627 rakwb per R5-920 Shahzad Rahmandoost verfasserin aut Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. Streptococcus mutans Glucosyltransferase gtfB gene Medicine R Medicine (General) Kumarss Amini verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 33(2015), 356, Seite 1804-1809 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:33 year:2015 number:356 pages:1804-1809 https://doaj.org/article/fac000a8242741779cad9ebbde7405af kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/4826 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 33 2015 356 1804-1809 |
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(DE-627)DOAJ06590303X (DE-599)DOAJfac000a8242741779cad9ebbde7405af DE-627 ger DE-627 rakwb per R5-920 Shahzad Rahmandoost verfasserin aut Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. Streptococcus mutans Glucosyltransferase gtfB gene Medicine R Medicine (General) Kumarss Amini verfasserin aut In مجله دانشکده پزشکی اصفهان Isfahan University of Medical Sciences, 2018 33(2015), 356, Seite 1804-1809 (DE-627)590956124 (DE-600)2476973-3 1735854X nnns volume:33 year:2015 number:356 pages:1804-1809 https://doaj.org/article/fac000a8242741779cad9ebbde7405af kostenfrei http://jims.mui.ac.ir/index.php/jims/article/view/4826 kostenfrei https://doaj.org/toc/1027-7595 Journal toc kostenfrei https://doaj.org/toc/1735-854X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 33 2015 356 1804-1809 |
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Identification of Streptococcus Mutans Isolated from Dental Plaques Based on the Presence of gtfB Gene |
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Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. |
abstractGer |
Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. |
abstract_unstemmed |
Background: Oral streptococci, especially Streptococcus mutans, are of the factors identified to cause tooth decay and periodontal disease. Glucosyltransferase gene (gtfB) encoded by the GTF-I is an important factor in the virulence of the bacteria. Now, the development of molecular methods tends to detect Streptococcus mutans with accuracy and speed. This study aimed to identify Streptococcus mutans in human dental plaques based on the presence of gtfB gene. Methods: After collecting 55 swab samples of dental plaques, biochemical and microbiological tests were performed. DNA samples were confirmed using the extraction kit and then, the polymerase chain reaction (PCR) assay was performed on samples to identify the gtfB gene. Findings: Of 55 isolates collected from dental plaques, 17 samples (30.9%) were positive for the presence of gtfB gene. Conclusion: The results obtained in this study found to have differences with other researches conducted in other countries and regions of Iran. These differences may be due to cultural differences in the use of toothbrush and dental floss, diet and oral health status. |
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|
score |
7.399988 |