Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation
Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present s...
Ausführliche Beschreibung
Autor*in: |
Anna Ochalek [verfasserIn] Balázs Mihalik [verfasserIn] Hasan X. Avci [verfasserIn] Abinaya Chandrasekaran [verfasserIn] Annamária Téglási [verfasserIn] István Bock [verfasserIn] Maria Lo Giudice [verfasserIn] Zsuzsanna Táncos [verfasserIn] Kinga Molnár [verfasserIn] Lajos László [verfasserIn] Jørgen E. Nielsen [verfasserIn] Bjørn Holst [verfasserIn] Kristine Freude [verfasserIn] Poul Hyttel [verfasserIn] Julianna Kobolák [verfasserIn] András Dinnyés [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2017 |
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In: Alzheimer’s Research & Therapy - BMC, 2015, 9(2017), 1, Seite 19 |
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Übergeordnetes Werk: |
volume:9 ; year:2017 ; number:1 ; pages:19 |
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DOI / URN: |
10.1186/s13195-017-0317-z |
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DOAJ06593721X |
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245 | 1 | 0 | |a Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation |
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520 | |a Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. | ||
650 | 4 | |a Familial Alzheimer’s disease | |
650 | 4 | |a Sporadic Alzheimer’s disease | |
650 | 4 | |a Induced pluripotent stem cells | |
650 | 4 | |a Amyloid β | |
650 | 4 | |a TAU pathology | |
650 | 4 | |a Hyperphosphorylation | |
653 | 0 | |a Neurosciences. Biological psychiatry. Neuropsychiatry | |
653 | 0 | |a Neurology. Diseases of the nervous system | |
700 | 0 | |a Balázs Mihalik |e verfasserin |4 aut | |
700 | 0 | |a Hasan X. Avci |e verfasserin |4 aut | |
700 | 0 | |a Abinaya Chandrasekaran |e verfasserin |4 aut | |
700 | 0 | |a Annamária Téglási |e verfasserin |4 aut | |
700 | 0 | |a István Bock |e verfasserin |4 aut | |
700 | 0 | |a Maria Lo Giudice |e verfasserin |4 aut | |
700 | 0 | |a Zsuzsanna Táncos |e verfasserin |4 aut | |
700 | 0 | |a Kinga Molnár |e verfasserin |4 aut | |
700 | 0 | |a Lajos László |e verfasserin |4 aut | |
700 | 0 | |a Jørgen E. Nielsen |e verfasserin |4 aut | |
700 | 0 | |a Bjørn Holst |e verfasserin |4 aut | |
700 | 0 | |a Kristine Freude |e verfasserin |4 aut | |
700 | 0 | |a Poul Hyttel |e verfasserin |4 aut | |
700 | 0 | |a Julianna Kobolák |e verfasserin |4 aut | |
700 | 0 | |a András Dinnyés |e verfasserin |4 aut | |
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10.1186/s13195-017-0317-z doi (DE-627)DOAJ06593721X (DE-599)DOAJ6f5f47269cee49599dfeeaaaeb8782f6 DE-627 ger DE-627 rakwb eng RC321-571 RC346-429 Anna Ochalek verfasserin aut Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation Neurosciences. Biological psychiatry. Neuropsychiatry Neurology. Diseases of the nervous system Balázs Mihalik verfasserin aut Hasan X. Avci verfasserin aut Abinaya Chandrasekaran verfasserin aut Annamária Téglási verfasserin aut István Bock verfasserin aut Maria Lo Giudice verfasserin aut Zsuzsanna Táncos verfasserin aut Kinga Molnár verfasserin aut Lajos László verfasserin aut Jørgen E. Nielsen verfasserin aut Bjørn Holst verfasserin aut Kristine Freude verfasserin aut Poul Hyttel verfasserin aut Julianna Kobolák verfasserin aut András Dinnyés verfasserin aut In Alzheimer’s Research & Therapy BMC, 2015 9(2017), 1, Seite 19 (DE-627)605683557 (DE-600)2506521-X 17589193 nnns volume:9 year:2017 number:1 pages:19 https://doi.org/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/article/6f5f47269cee49599dfeeaaaeb8782f6 kostenfrei http://link.springer.com/article/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/toc/1758-9193 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 1 19 |
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10.1186/s13195-017-0317-z doi (DE-627)DOAJ06593721X (DE-599)DOAJ6f5f47269cee49599dfeeaaaeb8782f6 DE-627 ger DE-627 rakwb eng RC321-571 RC346-429 Anna Ochalek verfasserin aut Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation Neurosciences. Biological psychiatry. Neuropsychiatry Neurology. Diseases of the nervous system Balázs Mihalik verfasserin aut Hasan X. Avci verfasserin aut Abinaya Chandrasekaran verfasserin aut Annamária Téglási verfasserin aut István Bock verfasserin aut Maria Lo Giudice verfasserin aut Zsuzsanna Táncos verfasserin aut Kinga Molnár verfasserin aut Lajos László verfasserin aut Jørgen E. Nielsen verfasserin aut Bjørn Holst verfasserin aut Kristine Freude verfasserin aut Poul Hyttel verfasserin aut Julianna Kobolák verfasserin aut András Dinnyés verfasserin aut In Alzheimer’s Research & Therapy BMC, 2015 9(2017), 1, Seite 19 (DE-627)605683557 (DE-600)2506521-X 17589193 nnns volume:9 year:2017 number:1 pages:19 https://doi.org/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/article/6f5f47269cee49599dfeeaaaeb8782f6 kostenfrei http://link.springer.com/article/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/toc/1758-9193 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 1 19 |
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10.1186/s13195-017-0317-z doi (DE-627)DOAJ06593721X (DE-599)DOAJ6f5f47269cee49599dfeeaaaeb8782f6 DE-627 ger DE-627 rakwb eng RC321-571 RC346-429 Anna Ochalek verfasserin aut Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation Neurosciences. Biological psychiatry. Neuropsychiatry Neurology. Diseases of the nervous system Balázs Mihalik verfasserin aut Hasan X. Avci verfasserin aut Abinaya Chandrasekaran verfasserin aut Annamária Téglási verfasserin aut István Bock verfasserin aut Maria Lo Giudice verfasserin aut Zsuzsanna Táncos verfasserin aut Kinga Molnár verfasserin aut Lajos László verfasserin aut Jørgen E. Nielsen verfasserin aut Bjørn Holst verfasserin aut Kristine Freude verfasserin aut Poul Hyttel verfasserin aut Julianna Kobolák verfasserin aut András Dinnyés verfasserin aut In Alzheimer’s Research & Therapy BMC, 2015 9(2017), 1, Seite 19 (DE-627)605683557 (DE-600)2506521-X 17589193 nnns volume:9 year:2017 number:1 pages:19 https://doi.org/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/article/6f5f47269cee49599dfeeaaaeb8782f6 kostenfrei http://link.springer.com/article/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/toc/1758-9193 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 1 19 |
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10.1186/s13195-017-0317-z doi (DE-627)DOAJ06593721X (DE-599)DOAJ6f5f47269cee49599dfeeaaaeb8782f6 DE-627 ger DE-627 rakwb eng RC321-571 RC346-429 Anna Ochalek verfasserin aut Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation Neurosciences. Biological psychiatry. Neuropsychiatry Neurology. Diseases of the nervous system Balázs Mihalik verfasserin aut Hasan X. Avci verfasserin aut Abinaya Chandrasekaran verfasserin aut Annamária Téglási verfasserin aut István Bock verfasserin aut Maria Lo Giudice verfasserin aut Zsuzsanna Táncos verfasserin aut Kinga Molnár verfasserin aut Lajos László verfasserin aut Jørgen E. Nielsen verfasserin aut Bjørn Holst verfasserin aut Kristine Freude verfasserin aut Poul Hyttel verfasserin aut Julianna Kobolák verfasserin aut András Dinnyés verfasserin aut In Alzheimer’s Research & Therapy BMC, 2015 9(2017), 1, Seite 19 (DE-627)605683557 (DE-600)2506521-X 17589193 nnns volume:9 year:2017 number:1 pages:19 https://doi.org/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/article/6f5f47269cee49599dfeeaaaeb8782f6 kostenfrei http://link.springer.com/article/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/toc/1758-9193 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 1 19 |
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10.1186/s13195-017-0317-z doi (DE-627)DOAJ06593721X (DE-599)DOAJ6f5f47269cee49599dfeeaaaeb8782f6 DE-627 ger DE-627 rakwb eng RC321-571 RC346-429 Anna Ochalek verfasserin aut Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation Neurosciences. Biological psychiatry. Neuropsychiatry Neurology. Diseases of the nervous system Balázs Mihalik verfasserin aut Hasan X. Avci verfasserin aut Abinaya Chandrasekaran verfasserin aut Annamária Téglási verfasserin aut István Bock verfasserin aut Maria Lo Giudice verfasserin aut Zsuzsanna Táncos verfasserin aut Kinga Molnár verfasserin aut Lajos László verfasserin aut Jørgen E. Nielsen verfasserin aut Bjørn Holst verfasserin aut Kristine Freude verfasserin aut Poul Hyttel verfasserin aut Julianna Kobolák verfasserin aut András Dinnyés verfasserin aut In Alzheimer’s Research & Therapy BMC, 2015 9(2017), 1, Seite 19 (DE-627)605683557 (DE-600)2506521-X 17589193 nnns volume:9 year:2017 number:1 pages:19 https://doi.org/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/article/6f5f47269cee49599dfeeaaaeb8782f6 kostenfrei http://link.springer.com/article/10.1186/s13195-017-0317-z kostenfrei https://doaj.org/toc/1758-9193 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2017 1 19 |
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Anna Ochalek @@aut@@ Balázs Mihalik @@aut@@ Hasan X. Avci @@aut@@ Abinaya Chandrasekaran @@aut@@ Annamária Téglási @@aut@@ István Bock @@aut@@ Maria Lo Giudice @@aut@@ Zsuzsanna Táncos @@aut@@ Kinga Molnár @@aut@@ Lajos László @@aut@@ Jørgen E. Nielsen @@aut@@ Bjørn Holst @@aut@@ Kristine Freude @@aut@@ Poul Hyttel @@aut@@ Julianna Kobolák @@aut@@ András Dinnyés @@aut@@ |
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Anna Ochalek misc RC321-571 misc RC346-429 misc Familial Alzheimer’s disease misc Sporadic Alzheimer’s disease misc Induced pluripotent stem cells misc Amyloid β misc TAU pathology misc Hyperphosphorylation misc Neurosciences. Biological psychiatry. Neuropsychiatry misc Neurology. Diseases of the nervous system Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation |
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RC321-571 RC346-429 Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation Familial Alzheimer’s disease Sporadic Alzheimer’s disease Induced pluripotent stem cells Amyloid β TAU pathology Hyperphosphorylation |
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Anna Ochalek Balázs Mihalik Hasan X. Avci Abinaya Chandrasekaran Annamária Téglási István Bock Maria Lo Giudice Zsuzsanna Táncos Kinga Molnár Lajos László Jørgen E. Nielsen Bjørn Holst Kristine Freude Poul Hyttel Julianna Kobolák András Dinnyés |
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Neurons derived from sporadic Alzheimer’s disease iPSCs reveal elevated TAU hyperphosphorylation, increased amyloid levels, and GSK3B activation |
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Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. |
abstractGer |
Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. |
abstract_unstemmed |
Abstract Background Alzheimer’s disease (AD) is the most common type of dementia, affecting one in eight adults over 65 years of age. The majority of AD cases are sporadic, with unknown etiology, and only 5% of all patients with AD present the familial monogenic form of the disease. In the present study, our aim was to establish an in vitro cell model based on patient-specific human neurons to study the pathomechanism of sporadic AD. Methods We compared neurons derived from induced pluripotent stem cell (iPSC) lines of patients with early-onset familial Alzheimer’s disease (fAD), all caused by mutations in the PSEN1 gene; patients with late-onset sporadic Alzheimer’s disease (sAD); and three control individuals without dementia. The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. Results Neurons from patients with fAD and patients with sAD showed increased phosphorylation of TAU protein at all investigated phosphorylation sites. Relative to the control neurons, neurons derived from patients with fAD and patients with sAD exhibited higher levels of extracellular amyloid-β 1–40 (Aβ1–40) and amyloid-β 1–42 (Aβ1–42). However, significantly increased Aβ1–42/Aβ1–40 ratios, which is one of the pathological markers of fAD, were observed only in samples of patients with fAD. Additionally, we detected increased levels of active glycogen synthase kinase 3 β, a physiological kinase of TAU, in neurons derived from AD iPSCs, as well as significant upregulation of amyloid precursor protein (APP) synthesis and APP carboxy-terminal fragment cleavage. Moreover, elevated sensitivity to oxidative stress, as induced by amyloid oligomers or peroxide, was detected in both fAD- and sAD-derived neurons. Conclusions On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ1–40 levels in phenotype between fAD and sAD samples. To our knowledge, this is the first study in which the hyperphosphorylation of TAU protein has been compared in fAD and sAD iPSC-derived neurons. Our findings demonstrate that iPSC technology is suitable to model both fAD and sAD and may provide a platform for developing new treatment strategies for these conditions. |
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