Evaluation of the leptin receptor in human spermatozoa

<p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempte...
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Autor*in:	Ghaedi Kamran [verfasserIn] Tanhaei Somayeh [verfasserIn] Tavalaee Marziyeh [verfasserIn] Zarkesh-Esfahani Hamid [verfasserIn] Razavi Shahnaz [verfasserIn] Hatami-Baroogh Leila [verfasserIn] Deemeh Mohamad [verfasserIn] Rabiee Farzaneh [verfasserIn] Nasr-Esfahani Mohammad [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: Reproductive Biology and Endocrinology - BMC, 2003, 8(2010), 1, p 17
Übergeordnetes Werk:	volume:8 ; year:2010 ; number:1, p 17

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1477-7827-8-17

Katalog-ID:	DOAJ066181666

Internformat


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520			\|a <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p<
653		0	\|a Gynecology and obstetrics
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700	0		\|a Zarkesh-Esfahani Hamid \|e verfasserin \|4 aut
700	0		\|a Razavi Shahnaz \|e verfasserin \|4 aut
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700	0		\|a Nasr-Esfahani Mohammad \|e verfasserin \|4 aut
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912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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author_variant	g k gk t s ts t m tm z e h zeh r s rs h b l hbl d m dm r f rf n e m nem
matchkey_str	article:14777827:2010----::vlainfhlpircpoih
hierarchy_sort_str	2010
callnumber-subject-code	RG
publishDate	2010
allfields	10.1186/1477-7827-8-17 doi (DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63 DE-627 ger DE-627 rakwb eng RG1-991 QH471-489 Ghaedi Kamran verfasserin aut Evaluation of the leptin receptor in human spermatozoa 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p< Gynecology and obstetrics Reproduction Tanhaei Somayeh verfasserin aut Tavalaee Marziyeh verfasserin aut Zarkesh-Esfahani Hamid verfasserin aut Razavi Shahnaz verfasserin aut Hatami-Baroogh Leila verfasserin aut Deemeh Mohamad verfasserin aut Rabiee Farzaneh verfasserin aut Nasr-Esfahani Mohammad verfasserin aut In Reproductive Biology and Endocrinology BMC, 2003 8(2010), 1, p 17 (DE-627)369554477 (DE-600)2119215-7 14777827 nnns volume:8 year:2010 number:1, p 17 https://doi.org/10.1186/1477-7827-8-17 kostenfrei https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 kostenfrei http://www.rbej.com/content/8/1/17 kostenfrei https://doaj.org/toc/1477-7827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1, p 17
spelling	10.1186/1477-7827-8-17 doi (DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63 DE-627 ger DE-627 rakwb eng RG1-991 QH471-489 Ghaedi Kamran verfasserin aut Evaluation of the leptin receptor in human spermatozoa 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p< Gynecology and obstetrics Reproduction Tanhaei Somayeh verfasserin aut Tavalaee Marziyeh verfasserin aut Zarkesh-Esfahani Hamid verfasserin aut Razavi Shahnaz verfasserin aut Hatami-Baroogh Leila verfasserin aut Deemeh Mohamad verfasserin aut Rabiee Farzaneh verfasserin aut Nasr-Esfahani Mohammad verfasserin aut In Reproductive Biology and Endocrinology BMC, 2003 8(2010), 1, p 17 (DE-627)369554477 (DE-600)2119215-7 14777827 nnns volume:8 year:2010 number:1, p 17 https://doi.org/10.1186/1477-7827-8-17 kostenfrei https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 kostenfrei http://www.rbej.com/content/8/1/17 kostenfrei https://doaj.org/toc/1477-7827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1, p 17
allfields_unstemmed	10.1186/1477-7827-8-17 doi (DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63 DE-627 ger DE-627 rakwb eng RG1-991 QH471-489 Ghaedi Kamran verfasserin aut Evaluation of the leptin receptor in human spermatozoa 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p< Gynecology and obstetrics Reproduction Tanhaei Somayeh verfasserin aut Tavalaee Marziyeh verfasserin aut Zarkesh-Esfahani Hamid verfasserin aut Razavi Shahnaz verfasserin aut Hatami-Baroogh Leila verfasserin aut Deemeh Mohamad verfasserin aut Rabiee Farzaneh verfasserin aut Nasr-Esfahani Mohammad verfasserin aut In Reproductive Biology and Endocrinology BMC, 2003 8(2010), 1, p 17 (DE-627)369554477 (DE-600)2119215-7 14777827 nnns volume:8 year:2010 number:1, p 17 https://doi.org/10.1186/1477-7827-8-17 kostenfrei https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 kostenfrei http://www.rbej.com/content/8/1/17 kostenfrei https://doaj.org/toc/1477-7827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1, p 17
allfieldsGer	10.1186/1477-7827-8-17 doi (DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63 DE-627 ger DE-627 rakwb eng RG1-991 QH471-489 Ghaedi Kamran verfasserin aut Evaluation of the leptin receptor in human spermatozoa 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p< Gynecology and obstetrics Reproduction Tanhaei Somayeh verfasserin aut Tavalaee Marziyeh verfasserin aut Zarkesh-Esfahani Hamid verfasserin aut Razavi Shahnaz verfasserin aut Hatami-Baroogh Leila verfasserin aut Deemeh Mohamad verfasserin aut Rabiee Farzaneh verfasserin aut Nasr-Esfahani Mohammad verfasserin aut In Reproductive Biology and Endocrinology BMC, 2003 8(2010), 1, p 17 (DE-627)369554477 (DE-600)2119215-7 14777827 nnns volume:8 year:2010 number:1, p 17 https://doi.org/10.1186/1477-7827-8-17 kostenfrei https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 kostenfrei http://www.rbej.com/content/8/1/17 kostenfrei https://doaj.org/toc/1477-7827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1, p 17
allfieldsSound	10.1186/1477-7827-8-17 doi (DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63 DE-627 ger DE-627 rakwb eng RG1-991 QH471-489 Ghaedi Kamran verfasserin aut Evaluation of the leptin receptor in human spermatozoa 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p< Gynecology and obstetrics Reproduction Tanhaei Somayeh verfasserin aut Tavalaee Marziyeh verfasserin aut Zarkesh-Esfahani Hamid verfasserin aut Razavi Shahnaz verfasserin aut Hatami-Baroogh Leila verfasserin aut Deemeh Mohamad verfasserin aut Rabiee Farzaneh verfasserin aut Nasr-Esfahani Mohammad verfasserin aut In Reproductive Biology and Endocrinology BMC, 2003 8(2010), 1, p 17 (DE-627)369554477 (DE-600)2119215-7 14777827 nnns volume:8 year:2010 number:1, p 17 https://doi.org/10.1186/1477-7827-8-17 kostenfrei https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 kostenfrei http://www.rbej.com/content/8/1/17 kostenfrei https://doaj.org/toc/1477-7827 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 1, p 17
language	English
source	In Reproductive Biology and Endocrinology 8(2010), 1, p 17 volume:8 year:2010 number:1, p 17
sourceStr	In Reproductive Biology and Endocrinology 8(2010), 1, p 17 volume:8 year:2010 number:1, p 17
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Gynecology and obstetrics Reproduction
isfreeaccess_bool	true
container_title	Reproductive Biology and Endocrinology
authorswithroles_txt_mv	Ghaedi Kamran @@aut@@ Tanhaei Somayeh @@aut@@ Tavalaee Marziyeh @@aut@@ Zarkesh-Esfahani Hamid @@aut@@ Razavi Shahnaz @@aut@@ Hatami-Baroogh Leila @@aut@@ Deemeh Mohamad @@aut@@ Rabiee Farzaneh @@aut@@ Nasr-Esfahani Mohammad @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	369554477
id	DOAJ066181666
language_de	englisch
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callnumber-first	R - Medicine
author	Ghaedi Kamran
spellingShingle	Ghaedi Kamran misc RG1-991 misc QH471-489 misc Gynecology and obstetrics misc Reproduction Evaluation of the leptin receptor in human spermatozoa
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topic_title	RG1-991 QH471-489 Evaluation of the leptin receptor in human spermatozoa
topic	misc RG1-991 misc QH471-489 misc Gynecology and obstetrics misc Reproduction
topic_unstemmed	misc RG1-991 misc QH471-489 misc Gynecology and obstetrics misc Reproduction
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title	Evaluation of the leptin receptor in human spermatozoa
ctrlnum	(DE-627)DOAJ066181666 (DE-599)DOAJc9eba8a8c6584a20abe69b92797c4f63
title_full	Evaluation of the leptin receptor in human spermatozoa
author_sort	Ghaedi Kamran
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journalStr	Reproductive Biology and Endocrinology
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author_browse	Ghaedi Kamran Tanhaei Somayeh Tavalaee Marziyeh Zarkesh-Esfahani Hamid Razavi Shahnaz Hatami-Baroogh Leila Deemeh Mohamad Rabiee Farzaneh Nasr-Esfahani Mohammad
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class	RG1-991 QH471-489
format_se	Elektronische Aufsätze
author-letter	Ghaedi Kamran
doi_str_mv	10.1186/1477-7827-8-17
author2-role	verfasserin
title_sort	evaluation of the leptin receptor in human spermatozoa
callnumber	RG1-991
title_auth	Evaluation of the leptin receptor in human spermatozoa
abstract	<p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Leptin, a 167 amino acid peptide hormone, profoundly effects reproduction exerting its biological effects via interaction with the leptin receptor (ObR) which is widely expressed on peripheral tissues. In this study, we have attempted to assess leptin receptor expression in the spermatozoa of fertile males and those diagnosed with male factor infertility; both at the mRNA or protein levels.</p< <p<Methods</p< <p<Semen samples were collected from fertile males and individuals with male factor infertility. In order to evaluate leptin receptor expression several techniques were utilized, including: reverse transcriptase-polymerase chain reaction (RT-PCR), immunostaining, flow cytometry, and western blotting. Mononuclear cells isolated from volunteers' peripheral blood were used as positive controls for leptin receptor expression.</p< <p<Results</p< <p<leptin receptor was noted on mononuclear cells but we were unable to detect this receptor on spermatozoa at the protein level. Leptin receptor expression was detected on peripheral blood mononuclear cells (PBMCs) as positive controls; however it was not detectable on the spermatozoa of both groups by immunofluorescence microscopy or flow cytometry. Furthermore, positive expression of the ObR long isoform as assessed by RT-PCR was observed in the sperm of only four cases, whereas expression of beta-Actin, a house keeping gene, and HspA2, a testis specific gene, was present in all cases.</p< <p<Conclusion</p< <p<The long isoform of leptin receptor may not be present on human sperm. Species difference may be accounted for diverse reproductive physiology which depends on metabolic requirement. Leptin receptor expression at the mRNA level in some individuals may be related to contamination by other cells in semen.</p<
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title_short	Evaluation of the leptin receptor in human spermatozoa
url	https://doi.org/10.1186/1477-7827-8-17 https://doaj.org/article/c9eba8a8c6584a20abe69b92797c4f63 http://www.rbej.com/content/8/1/17 https://doaj.org/toc/1477-7827
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