Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector

Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, an...
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Gespeichert in:

Autor*in:	Salman Asghari [verfasserIn] Mahmoud Shekari Khaniani [verfasserIn] Masood Darabi [verfasserIn] Sima Mansoori Derakhshan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Human SCF Cloning pET-26b(+)

Übergeordnetes Werk:	In: Advanced Pharmaceutical Bulletin - Tabriz University of Medical Sciences, 2011, 4(2014), 1, Seite 91-95
Übergeordnetes Werk:	volume:4 ; year:2014 ; number:1 ; pages:91-95

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5681/apb.2014.014

Katalog-ID:	DOAJ066364299

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allfields_unstemmed	10.5681/apb.2014.014 doi (DE-627)DOAJ066364299 (DE-599)DOAJ71cb0a6e88f6446c9183ce39c6a8cb25 DE-627 ger DE-627 rakwb eng RM1-950 Salman Asghari verfasserin aut Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein. Human SCF Cloning pET-26b(+) Therapeutics. Pharmacology Mahmoud Shekari Khaniani verfasserin aut Masood Darabi verfasserin aut Sima Mansoori Derakhshan verfasserin aut In Advanced Pharmaceutical Bulletin Tabriz University of Medical Sciences, 2011 4(2014), 1, Seite 91-95 (DE-627)169603163X 22517308 nnns volume:4 year:2014 number:1 pages:91-95 https://doi.org/10.5681/apb.2014.014 kostenfrei https://doaj.org/article/71cb0a6e88f6446c9183ce39c6a8cb25 kostenfrei http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-91.pdf kostenfrei https://doaj.org/toc/2228-5881 Journal toc kostenfrei https://doaj.org/toc/2251-7308 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 1 91-95
allfieldsGer	10.5681/apb.2014.014 doi (DE-627)DOAJ066364299 (DE-599)DOAJ71cb0a6e88f6446c9183ce39c6a8cb25 DE-627 ger DE-627 rakwb eng RM1-950 Salman Asghari verfasserin aut Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein. Human SCF Cloning pET-26b(+) Therapeutics. Pharmacology Mahmoud Shekari Khaniani verfasserin aut Masood Darabi verfasserin aut Sima Mansoori Derakhshan verfasserin aut In Advanced Pharmaceutical Bulletin Tabriz University of Medical Sciences, 2011 4(2014), 1, Seite 91-95 (DE-627)169603163X 22517308 nnns volume:4 year:2014 number:1 pages:91-95 https://doi.org/10.5681/apb.2014.014 kostenfrei https://doaj.org/article/71cb0a6e88f6446c9183ce39c6a8cb25 kostenfrei http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-91.pdf kostenfrei https://doaj.org/toc/2228-5881 Journal toc kostenfrei https://doaj.org/toc/2251-7308 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 1 91-95
allfieldsSound	10.5681/apb.2014.014 doi (DE-627)DOAJ066364299 (DE-599)DOAJ71cb0a6e88f6446c9183ce39c6a8cb25 DE-627 ger DE-627 rakwb eng RM1-950 Salman Asghari verfasserin aut Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein. Human SCF Cloning pET-26b(+) Therapeutics. Pharmacology Mahmoud Shekari Khaniani verfasserin aut Masood Darabi verfasserin aut Sima Mansoori Derakhshan verfasserin aut In Advanced Pharmaceutical Bulletin Tabriz University of Medical Sciences, 2011 4(2014), 1, Seite 91-95 (DE-627)169603163X 22517308 nnns volume:4 year:2014 number:1 pages:91-95 https://doi.org/10.5681/apb.2014.014 kostenfrei https://doaj.org/article/71cb0a6e88f6446c9183ce39c6a8cb25 kostenfrei http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-91.pdf kostenfrei https://doaj.org/toc/2228-5881 Journal toc kostenfrei https://doaj.org/toc/2251-7308 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 4 2014 1 91-95
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callnumber-first	R - Medicine
author	Salman Asghari
spellingShingle	Salman Asghari misc RM1-950 misc Human SCF misc Cloning misc pET-26b(+) misc Therapeutics. Pharmacology Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
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topic_title	RM1-950 Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector Human SCF Cloning pET-26b(+)
topic	misc RM1-950 misc Human SCF misc Cloning misc pET-26b(+) misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc Human SCF misc Cloning misc pET-26b(+) misc Therapeutics. Pharmacology
topic_browse	misc RM1-950 misc Human SCF misc Cloning misc pET-26b(+) misc Therapeutics. Pharmacology
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title	Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
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title_full	Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
author_sort	Salman Asghari
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author_browse	Salman Asghari Mahmoud Shekari Khaniani Masood Darabi Sima Mansoori Derakhshan
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author-letter	Salman Asghari
doi_str_mv	10.5681/apb.2014.014
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title_sort	cloning of soluble human stem cell factor in pet-26b(+) vector
callnumber	RM1-950
title_auth	Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
abstract	Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein.
abstractGer	Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein.
abstract_unstemmed	Purpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein.
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title_short	Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector
url	https://doi.org/10.5681/apb.2014.014 https://doaj.org/article/71cb0a6e88f6446c9183ce39c6a8cb25 http://journals.tbzmed.ac.ir/APB/Manuscript/APB-4-91.pdf https://doaj.org/toc/2228-5881 https://doaj.org/toc/2251-7308
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up_date	2024-07-03T19:39:23.563Z
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Cloning of Soluble Human Stem Cell Factor in pET-26b(+) Vector

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