Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)

A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for plur...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Ying Wang [verfasserIn] Qiang Fen [verfasserIn] Hongxiang Yu [verfasserIn] Haixia Qiu [verfasserIn] Xiquan Ma [verfasserIn] Ying Lei [verfasserIn] Jian Zhao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Übergeordnetes Werk:	In: Stem Cell Research - Elsevier, 2015, 33(2018), Seite 65-68
Übergeordnetes Werk:	volume:33 ; year:2018 ; pages:65-68

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.scr.2018.10.014

Katalog-ID:	DOAJ066418712

Internformat


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520			\|a A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1).
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700	0		\|a Jian Zhao \|e verfasserin \|4 aut
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912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
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912			\|a GBV_ILN_2007
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912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
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912			\|a GBV_ILN_2056
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912			\|a GBV_ILN_2061
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912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
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912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
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publishDate	2018
allfields	10.1016/j.scr.2018.10.014 doi (DE-627)DOAJ066418712 (DE-599)DOAJ1357e35f3e76492e91ad9c5b24c20182 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Wang verfasserin aut Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1). Biology (General) Qiang Fen verfasserin aut Hongxiang Yu verfasserin aut Haixia Qiu verfasserin aut Xiquan Ma verfasserin aut Ying Lei verfasserin aut Jian Zhao verfasserin aut In Stem Cell Research Elsevier, 2015 33(2018), Seite 65-68 (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:33 year:2018 pages:65-68 https://doi.org/10.1016/j.scr.2018.10.014 kostenfrei https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506118302502 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 33 2018 65-68
spelling	10.1016/j.scr.2018.10.014 doi (DE-627)DOAJ066418712 (DE-599)DOAJ1357e35f3e76492e91ad9c5b24c20182 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Wang verfasserin aut Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1). Biology (General) Qiang Fen verfasserin aut Hongxiang Yu verfasserin aut Haixia Qiu verfasserin aut Xiquan Ma verfasserin aut Ying Lei verfasserin aut Jian Zhao verfasserin aut In Stem Cell Research Elsevier, 2015 33(2018), Seite 65-68 (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:33 year:2018 pages:65-68 https://doi.org/10.1016/j.scr.2018.10.014 kostenfrei https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506118302502 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 33 2018 65-68
allfields_unstemmed	10.1016/j.scr.2018.10.014 doi (DE-627)DOAJ066418712 (DE-599)DOAJ1357e35f3e76492e91ad9c5b24c20182 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Wang verfasserin aut Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1). Biology (General) Qiang Fen verfasserin aut Hongxiang Yu verfasserin aut Haixia Qiu verfasserin aut Xiquan Ma verfasserin aut Ying Lei verfasserin aut Jian Zhao verfasserin aut In Stem Cell Research Elsevier, 2015 33(2018), Seite 65-68 (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:33 year:2018 pages:65-68 https://doi.org/10.1016/j.scr.2018.10.014 kostenfrei https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506118302502 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 33 2018 65-68
allfieldsGer	10.1016/j.scr.2018.10.014 doi (DE-627)DOAJ066418712 (DE-599)DOAJ1357e35f3e76492e91ad9c5b24c20182 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Wang verfasserin aut Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1). Biology (General) Qiang Fen verfasserin aut Hongxiang Yu verfasserin aut Haixia Qiu verfasserin aut Xiquan Ma verfasserin aut Ying Lei verfasserin aut Jian Zhao verfasserin aut In Stem Cell Research Elsevier, 2015 33(2018), Seite 65-68 (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:33 year:2018 pages:65-68 https://doi.org/10.1016/j.scr.2018.10.014 kostenfrei https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506118302502 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 33 2018 65-68
allfieldsSound	10.1016/j.scr.2018.10.014 doi (DE-627)DOAJ066418712 (DE-599)DOAJ1357e35f3e76492e91ad9c5b24c20182 DE-627 ger DE-627 rakwb eng QH301-705.5 Ying Wang verfasserin aut Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1). Biology (General) Qiang Fen verfasserin aut Hongxiang Yu verfasserin aut Haixia Qiu verfasserin aut Xiquan Ma verfasserin aut Ying Lei verfasserin aut Jian Zhao verfasserin aut In Stem Cell Research Elsevier, 2015 33(2018), Seite 65-68 (DE-627)548125856 (DE-600)2393143-7 18767753 nnns volume:33 year:2018 pages:65-68 https://doi.org/10.1016/j.scr.2018.10.014 kostenfrei https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 kostenfrei http://www.sciencedirect.com/science/article/pii/S1873506118302502 kostenfrei https://doaj.org/toc/1873-5061 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 33 2018 65-68
language	English
source	In Stem Cell Research 33(2018), Seite 65-68 volume:33 year:2018 pages:65-68
sourceStr	In Stem Cell Research 33(2018), Seite 65-68 volume:33 year:2018 pages:65-68
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biology (General)
isfreeaccess_bool	true
container_title	Stem Cell Research
authorswithroles_txt_mv	Ying Wang @@aut@@ Qiang Fen @@aut@@ Hongxiang Yu @@aut@@ Haixia Qiu @@aut@@ Xiquan Ma @@aut@@ Ying Lei @@aut@@ Jian Zhao @@aut@@
publishDateDaySort_date	2018-01-01T00:00:00Z
hierarchy_top_id	548125856
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Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. 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callnumber-first	Q - Science
author	Ying Wang
spellingShingle	Ying Wang misc QH301-705.5 misc Biology (General) Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
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topic_title	QH301-705.5 Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
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title	Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
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title_full	Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
author_sort	Ying Wang
journal	Stem Cell Research
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author_browse	Ying Wang Qiang Fen Hongxiang Yu Haixia Qiu Xiquan Ma Ying Lei Jian Zhao
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title_sort	establishment of tusmi005-a, an induced pluripotent stem cell (ipsc) line from a 32-year old chinese han patient with bipolar disorder (bd)
callnumber	QH301-705.5
title_auth	Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
abstract	A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1).
abstractGer	A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1).
abstract_unstemmed	A 32-year old Bipolar Disorder (BD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG).016.01.1).
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title_short	Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)
url	https://doi.org/10.1016/j.scr.2018.10.014 https://doaj.org/article/1357e35f3e76492e91ad9c5b24c20182 http://www.sciencedirect.com/science/article/pii/S1873506118302502 https://doaj.org/toc/1873-5061
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up_date	2024-07-03T19:56:47.701Z
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The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in BD.Resource tableUnlabelled TableUnique stem cell line identifierTUSMi005-AAlternative name(s) of stem cell lineN/AInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell sourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic modificationNOType of modificationN/AAssociated diseaseBipolar Disorder (BD)Gene/locusSLC1A3/chr 5p13.2: 36679820, rs117588697 A-G,MAPT/chr 17q21.31:44073818, rs577501443 G-A,SIRT1/chr 10q21.3: 67864422, rs12415800 G-AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi005-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. 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Establishment of TUSMi005-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Bipolar Disorder (BD)

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