Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification

A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was design...
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Gespeichert in:

Autor*in:	Byung-Yong PARK [verfasserIn] Kwan-Seob SHIM [verfasserIn] Won-Il KIM [verfasserIn] Md Mukter HOSSAIN [verfasserIn] Bumseok KIM [verfasserIn] Jungkee KWON [verfasserIn] Choi-Kyu PARK [verfasserIn] Sung-Jin CHO [verfasserIn] Inho JO [verfasserIn] Ho-Seong CHO [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification

Übergeordnetes Werk:	In: Acta Veterinaria - Sciendo, 2010, 65(2015), 1, Seite 20-29
Übergeordnetes Werk:	volume:65 ; year:2015 ; number:1 ; pages:20-29

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1515/acve-2015-0002

Katalog-ID:	DOAJ066748208

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spelling	10.1515/acve-2015-0002 doi (DE-627)DOAJ066748208 (DE-599)DOAJ3e86de5e9f9c413c9ac08105b79be3c1 DE-627 ger DE-627 rakwb eng SF600-1100 Byung-Yong PARK verfasserin aut Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples. lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine Kwan-Seob SHIM verfasserin aut Won-Il KIM verfasserin aut Md Mukter HOSSAIN verfasserin aut Bumseok KIM verfasserin aut Jungkee KWON verfasserin aut Choi-Kyu PARK verfasserin aut Sung-Jin CHO verfasserin aut Inho JO verfasserin aut Ho-Seong CHO verfasserin aut In Acta Veterinaria Sciendo, 2010 65(2015), 1, Seite 20-29 (DE-627)538997877 (DE-600)2380825-1 18207448 nnns volume:65 year:2015 number:1 pages:20-29 https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1 kostenfrei https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/toc/1820-7448 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 65 2015 1 20-29
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allfieldsGer	10.1515/acve-2015-0002 doi (DE-627)DOAJ066748208 (DE-599)DOAJ3e86de5e9f9c413c9ac08105b79be3c1 DE-627 ger DE-627 rakwb eng SF600-1100 Byung-Yong PARK verfasserin aut Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples. lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine Kwan-Seob SHIM verfasserin aut Won-Il KIM verfasserin aut Md Mukter HOSSAIN verfasserin aut Bumseok KIM verfasserin aut Jungkee KWON verfasserin aut Choi-Kyu PARK verfasserin aut Sung-Jin CHO verfasserin aut Inho JO verfasserin aut Ho-Seong CHO verfasserin aut In Acta Veterinaria Sciendo, 2010 65(2015), 1, Seite 20-29 (DE-627)538997877 (DE-600)2380825-1 18207448 nnns volume:65 year:2015 number:1 pages:20-29 https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1 kostenfrei https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/toc/1820-7448 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 65 2015 1 20-29
allfieldsSound	10.1515/acve-2015-0002 doi (DE-627)DOAJ066748208 (DE-599)DOAJ3e86de5e9f9c413c9ac08105b79be3c1 DE-627 ger DE-627 rakwb eng SF600-1100 Byung-Yong PARK verfasserin aut Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples. lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification Veterinary medicine Kwan-Seob SHIM verfasserin aut Won-Il KIM verfasserin aut Md Mukter HOSSAIN verfasserin aut Bumseok KIM verfasserin aut Jungkee KWON verfasserin aut Choi-Kyu PARK verfasserin aut Sung-Jin CHO verfasserin aut Inho JO verfasserin aut Ho-Seong CHO verfasserin aut In Acta Veterinaria Sciendo, 2010 65(2015), 1, Seite 20-29 (DE-627)538997877 (DE-600)2380825-1 18207448 nnns volume:65 year:2015 number:1 pages:20-29 https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1 kostenfrei https://doi.org/10.1515/acve-2015-0002 kostenfrei https://doaj.org/toc/1820-7448 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 65 2015 1 20-29
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source	In Acta Veterinaria 65(2015), 1, Seite 20-29 volume:65 year:2015 number:1 pages:20-29
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callnumber-first	S - Agriculture
author	Byung-Yong PARK
spellingShingle	Byung-Yong PARK misc SF600-1100 misc lawsonia intracellularis misc pig misc porcine proliferative enteropathy misc real-time loopmediated isothermal amplification misc Veterinary medicine Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
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topic_title	SF600-1100 Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification lawsonia intracellularis pig porcine proliferative enteropathy real-time loopmediated isothermal amplification
topic	misc SF600-1100 misc lawsonia intracellularis misc pig misc porcine proliferative enteropathy misc real-time loopmediated isothermal amplification misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc lawsonia intracellularis misc pig misc porcine proliferative enteropathy misc real-time loopmediated isothermal amplification misc Veterinary medicine
topic_browse	misc SF600-1100 misc lawsonia intracellularis misc pig misc porcine proliferative enteropathy misc real-time loopmediated isothermal amplification misc Veterinary medicine
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title	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
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title_full	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
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author_browse	Byung-Yong PARK Kwan-Seob SHIM Won-Il KIM Md Mukter HOSSAIN Bumseok KIM Jungkee KWON Choi-Kyu PARK Sung-Jin CHO Inho JO Ho-Seong CHO
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title_sort	rapid and sensitive detection of lawsonia intracellularis in pigs by real-time loop-mediated isothermal amplification
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title_auth	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
abstract	A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
abstractGer	A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
abstract_unstemmed	A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.
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title_short	Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification
url	https://doi.org/10.1515/acve-2015-0002 https://doaj.org/article/3e86de5e9f9c413c9ac08105b79be3c1 https://doaj.org/toc/1820-7448
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up_date	2024-07-03T21:46:54.323Z
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Rapid And Sensitive Detection Of Lawsonia Intracellularis In Pigs By Real-Time Loop-Mediated Isothermal Amplification

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