Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome

BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined...
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Autor*in:	Chaojie Yu [verfasserIn] Xinli Zhan [verfasserIn] Tuo Liang [verfasserIn] Liyi Chen [verfasserIn] Zide Zhang [verfasserIn] Jie Jiang [verfasserIn] Jiang Xue [verfasserIn] Jiarui Chen [verfasserIn] Chong Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome

Übergeordnetes Werk:	In: Frontiers in Immunology - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fimmu.2021.572592

Katalog-ID:	DOAJ066782988

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520			\|a BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
650		4	\|a ankylosing spondylitis
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allfields	10.3389/fimmu.2021.572592 doi (DE-627)DOAJ066782988 (DE-599)DOAJ1cf0c3b335304580915fad2224613ceb DE-627 ger DE-627 rakwb eng RC581-607 Chaojie Yu verfasserin aut Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway. ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy Xinli Zhan verfasserin aut Tuo Liang verfasserin aut Liyi Chen verfasserin aut Zide Zhang verfasserin aut Jie Jiang verfasserin aut Jiang Xue verfasserin aut Jiarui Chen verfasserin aut Chong Liu verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.572592 kostenfrei https://doaj.org/article/1cf0c3b335304580915fad2224613ceb kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
spelling	10.3389/fimmu.2021.572592 doi (DE-627)DOAJ066782988 (DE-599)DOAJ1cf0c3b335304580915fad2224613ceb DE-627 ger DE-627 rakwb eng RC581-607 Chaojie Yu verfasserin aut Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway. ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy Xinli Zhan verfasserin aut Tuo Liang verfasserin aut Liyi Chen verfasserin aut Zide Zhang verfasserin aut Jie Jiang verfasserin aut Jiang Xue verfasserin aut Jiarui Chen verfasserin aut Chong Liu verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.572592 kostenfrei https://doaj.org/article/1cf0c3b335304580915fad2224613ceb kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfields_unstemmed	10.3389/fimmu.2021.572592 doi (DE-627)DOAJ066782988 (DE-599)DOAJ1cf0c3b335304580915fad2224613ceb DE-627 ger DE-627 rakwb eng RC581-607 Chaojie Yu verfasserin aut Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway. ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy Xinli Zhan verfasserin aut Tuo Liang verfasserin aut Liyi Chen verfasserin aut Zide Zhang verfasserin aut Jie Jiang verfasserin aut Jiang Xue verfasserin aut Jiarui Chen verfasserin aut Chong Liu verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.572592 kostenfrei https://doaj.org/article/1cf0c3b335304580915fad2224613ceb kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsGer	10.3389/fimmu.2021.572592 doi (DE-627)DOAJ066782988 (DE-599)DOAJ1cf0c3b335304580915fad2224613ceb DE-627 ger DE-627 rakwb eng RC581-607 Chaojie Yu verfasserin aut Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway. ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy Xinli Zhan verfasserin aut Tuo Liang verfasserin aut Liyi Chen verfasserin aut Zide Zhang verfasserin aut Jie Jiang verfasserin aut Jiang Xue verfasserin aut Jiarui Chen verfasserin aut Chong Liu verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.572592 kostenfrei https://doaj.org/article/1cf0c3b335304580915fad2224613ceb kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsSound	10.3389/fimmu.2021.572592 doi (DE-627)DOAJ066782988 (DE-599)DOAJ1cf0c3b335304580915fad2224613ceb DE-627 ger DE-627 rakwb eng RC581-607 Chaojie Yu verfasserin aut Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway. ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy Xinli Zhan verfasserin aut Tuo Liang verfasserin aut Liyi Chen verfasserin aut Zide Zhang verfasserin aut Jie Jiang verfasserin aut Jiang Xue verfasserin aut Jiarui Chen verfasserin aut Chong Liu verfasserin aut In Frontiers in Immunology Frontiers Media S.A., 2011 12(2021) (DE-627)657998354 (DE-600)2606827-8 16643224 nnns volume:12 year:2021 https://doi.org/10.3389/fimmu.2021.572592 kostenfrei https://doaj.org/article/1cf0c3b335304580915fad2224613ceb kostenfrei https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full kostenfrei https://doaj.org/toc/1664-3224 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
language	English
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topic_facet	ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome Immunologic diseases. Allergy
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container_title	Frontiers in Immunology
authorswithroles_txt_mv	Chaojie Yu @@aut@@ Xinli Zhan @@aut@@ Tuo Liang @@aut@@ Liyi Chen @@aut@@ Zide Zhang @@aut@@ Jie Jiang @@aut@@ Jiang Xue @@aut@@ Jiarui Chen @@aut@@ Chong Liu @@aut@@
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The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ankylosing spondylitis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">proteomics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bioinformatics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">myeloperoxidase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">phagosome</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Immunologic diseases. 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callnumber-first	R - Medicine
author	Chaojie Yu
spellingShingle	Chaojie Yu misc RC581-607 misc ankylosing spondylitis misc proteomics misc bioinformatics misc myeloperoxidase misc phagosome misc Immunologic diseases. Allergy Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
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topic_title	RC581-607 Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome ankylosing spondylitis proteomics bioinformatics myeloperoxidase phagosome
topic	misc RC581-607 misc ankylosing spondylitis misc proteomics misc bioinformatics misc myeloperoxidase misc phagosome misc Immunologic diseases. Allergy
topic_unstemmed	misc RC581-607 misc ankylosing spondylitis misc proteomics misc bioinformatics misc myeloperoxidase misc phagosome misc Immunologic diseases. Allergy
topic_browse	misc RC581-607 misc ankylosing spondylitis misc proteomics misc bioinformatics misc myeloperoxidase misc phagosome misc Immunologic diseases. Allergy
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title	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
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title_full	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
author_sort	Chaojie Yu
journal	Frontiers in Immunology
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author_browse	Chaojie Yu Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu
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title_sort	mechanism of hip arthropathy in ankylosing spondylitis: abnormal myeloperoxidase and phagosome
callnumber	RC581-607
title_auth	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
abstract	BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
abstractGer	BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
abstract_unstemmed	BackgroundThe pathogenesis of Ankylosing spondylitis (AS) has not been elucidated, especially involving hip joint disease. The purpose of this study was to analyze the proteome of diseased hip in AS and to identify key protein biomarkers.Material and MethodsWe used label-free quantification combined with liquid chromatography mass spectrometry (LC–MS/MS) to screen for differentially expressed proteins in hip ligament samples between AS and No-AS groups. Key protein was screened by Bioinformatics methods. and verified by in vitro experiments.ResultsThere were 3,755 identified proteins, of which 92.916% were quantified. A total of 193 DEPs (49 upregulated proteins and 144 downregulated proteins) were identified according to P < 0.01 and Log\|FC\| > 1. DEPs were mainly involved in cell compartment, including the vacuolar lumen, azurophil granule, primary lysosome, etc. The main KEGG pathway included Phagosome, Glycerophospholipid metabolism, Lysine degradation, Pentose phosphate pathway. Myeloperoxidase (MPO) was identified as a key protein involved in Phagosome pathway. The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.
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title_short	Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome
url	https://doi.org/10.3389/fimmu.2021.572592 https://doaj.org/article/1cf0c3b335304580915fad2224613ceb https://www.frontiersin.org/articles/10.3389/fimmu.2021.572592/full https://doaj.org/toc/1664-3224
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author2	Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu
author2Str	Xinli Zhan Tuo Liang Liyi Chen Zide Zhang Jie Jiang Jiang Xue Jiarui Chen Chong Liu
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up_date	2024-07-03T21:58:32.023Z
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The experiment of siRNA interfering with cells further confirmed that the upregulated MPO may promote the inflammatory response of fibroblasts.ConclusionsThe overexpression of MPO may contribute to the autoimmune inflammatory response of AS-affected hip joint through the phagosome pathway.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ankylosing spondylitis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">proteomics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bioinformatics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">myeloperoxidase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">phagosome</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Immunologic diseases. 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Mechanism of Hip Arthropathy in Ankylosing Spondylitis: Abnormal Myeloperoxidase and Phagosome

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