A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules

A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically dete...
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Autor*in:	Hildgund Schrempf [verfasserIn] Marta Kotasinska [verfasserIn] Yury Herasimenka [verfasserIn] Stefan Walter [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	chitin-assay chitin-binding protein chitin synthase electron microscopy polysaccharides

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 11(2010), 9, Seite 3122-3137
Übergeordnetes Werk:	volume:11 ; year:2010 ; number:9 ; pages:3122-3137

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms11093122

Katalog-ID:	DOAJ067160956

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allfieldsSound	10.3390/ijms11093122 doi (DE-627)DOAJ067160956 (DE-599)DOAJ06cbb6648c324715a5767af3bea97531 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hildgund Schrempf verfasserin aut A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis) has been established. Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein), has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes. chitin-assay chitin-binding protein chitin synthase electron microscopy polysaccharides Biology (General) Chemistry Marta Kotasinska verfasserin aut Yury Herasimenka verfasserin aut Stefan Walter verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 11(2010), 9, Seite 3122-3137 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:11 year:2010 number:9 pages:3122-3137 https://doi.org/10.3390/ijms11093122 kostenfrei https://doaj.org/article/06cbb6648c324715a5767af3bea97531 kostenfrei http://www.mdpi.com/1422-0067/11/9/3122/ kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 9 3122-3137
language	English
source	In International Journal of Molecular Sciences 11(2010), 9, Seite 3122-3137 volume:11 year:2010 number:9 pages:3122-3137
sourceStr	In International Journal of Molecular Sciences 11(2010), 9, Seite 3122-3137 volume:11 year:2010 number:9 pages:3122-3137
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topic_facet	chitin-assay chitin-binding protein chitin synthase electron microscopy polysaccharides Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Hildgund Schrempf @@aut@@ Marta Kotasinska @@aut@@ Yury Herasimenka @@aut@@ Stefan Walter @@aut@@
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callnumber-first	Q - Science
author	Hildgund Schrempf
spellingShingle	Hildgund Schrempf misc QH301-705.5 misc QD1-999 misc chitin-assay misc chitin-binding protein misc chitin synthase misc electron microscopy misc polysaccharides misc Biology (General) misc Chemistry A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules
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topic_title	QH301-705.5 QD1-999 A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules chitin-assay chitin-binding protein chitin synthase electron microscopy polysaccharides
topic	misc QH301-705.5 misc QD1-999 misc chitin-assay misc chitin-binding protein misc chitin synthase misc electron microscopy misc polysaccharides misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc chitin-assay misc chitin-binding protein misc chitin synthase misc electron microscopy misc polysaccharides misc Biology (General) misc Chemistry
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title	A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules
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title_full	A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules
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title_sort	selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules
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title_auth	A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules
abstract	A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis) has been established. Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein), has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes.
abstractGer	A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis) has been established. Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein), has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes.
abstract_unstemmed	A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable. In addition, the assay allows rapid scoring of the activity of chitin-synthases. As a result, a refined procedure for the rapid purification of yeast chitosomes (nano-machineries for chitin biosynthesis) has been established. Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein), has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes.
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title_short	A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules
url	https://doi.org/10.3390/ijms11093122 https://doaj.org/article/06cbb6648c324715a5767af3bea97531 http://www.mdpi.com/1422-0067/11/9/3122/ https://doaj.org/toc/1422-0067
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A Selective Assay to Detect Chitin and Biologically Active Nano-Machineries for Chitin-Biosynthesis with Their Intrinsic Chitin-Synthase Molecules

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