Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus

A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this...
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Autor*in:	A. Manicavasaka Dinakaran [verfasserIn] G. Selvaraju [verfasserIn] K. Jayalakshmi [verfasserIn] T.R. Gopalakrishna Murthy [verfasserIn] M. Geetha and S. Saravanan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test

Übergeordnetes Werk:	In: Veterinary World - Veterinary World, 2011, 3(2010), 5.000, Seite 212-214
Übergeordnetes Werk:	volume:3 ; year:2010 ; number:5.000 ; pages:212-214

Links:	Link aufrufen Link aufrufen Journal toc

Katalog-ID:	DOAJ067331467

Internformat


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520			\|a A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214]
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allfields	(DE-627)DOAJ067331467 (DE-599)DOAJ55d8bb49533f48489f1e81c4aadb443b DE-627 ger DE-627 rakwb eng SF1-1100 SF600-1100 A. Manicavasaka Dinakaran verfasserin aut Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214] Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine G. Selvaraju verfasserin aut K. Jayalakshmi verfasserin aut T.R. Gopalakrishna Murthy verfasserin aut M. Geetha and S. Saravanan verfasserin aut In Veterinary World Veterinary World, 2011 3(2010), 5.000, Seite 212-214 (DE-627)582020751 (DE-600)2456277-4 22310916 nnns volume:3 year:2010 number:5.000 pages:212-214 https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b kostenfrei http://www.scopemed.org/mnstemps/2/2-1296640457.pdf kostenfrei https://doaj.org/toc/0972-8988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2010 5.000 212-214
spelling	(DE-627)DOAJ067331467 (DE-599)DOAJ55d8bb49533f48489f1e81c4aadb443b DE-627 ger DE-627 rakwb eng SF1-1100 SF600-1100 A. Manicavasaka Dinakaran verfasserin aut Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214] Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine G. Selvaraju verfasserin aut K. Jayalakshmi verfasserin aut T.R. Gopalakrishna Murthy verfasserin aut M. Geetha and S. Saravanan verfasserin aut In Veterinary World Veterinary World, 2011 3(2010), 5.000, Seite 212-214 (DE-627)582020751 (DE-600)2456277-4 22310916 nnns volume:3 year:2010 number:5.000 pages:212-214 https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b kostenfrei http://www.scopemed.org/mnstemps/2/2-1296640457.pdf kostenfrei https://doaj.org/toc/0972-8988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2010 5.000 212-214
allfields_unstemmed	(DE-627)DOAJ067331467 (DE-599)DOAJ55d8bb49533f48489f1e81c4aadb443b DE-627 ger DE-627 rakwb eng SF1-1100 SF600-1100 A. Manicavasaka Dinakaran verfasserin aut Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214] Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine G. Selvaraju verfasserin aut K. Jayalakshmi verfasserin aut T.R. Gopalakrishna Murthy verfasserin aut M. Geetha and S. Saravanan verfasserin aut In Veterinary World Veterinary World, 2011 3(2010), 5.000, Seite 212-214 (DE-627)582020751 (DE-600)2456277-4 22310916 nnns volume:3 year:2010 number:5.000 pages:212-214 https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b kostenfrei http://www.scopemed.org/mnstemps/2/2-1296640457.pdf kostenfrei https://doaj.org/toc/0972-8988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2010 5.000 212-214
allfieldsGer	(DE-627)DOAJ067331467 (DE-599)DOAJ55d8bb49533f48489f1e81c4aadb443b DE-627 ger DE-627 rakwb eng SF1-1100 SF600-1100 A. Manicavasaka Dinakaran verfasserin aut Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214] Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine G. Selvaraju verfasserin aut K. Jayalakshmi verfasserin aut T.R. Gopalakrishna Murthy verfasserin aut M. Geetha and S. Saravanan verfasserin aut In Veterinary World Veterinary World, 2011 3(2010), 5.000, Seite 212-214 (DE-627)582020751 (DE-600)2456277-4 22310916 nnns volume:3 year:2010 number:5.000 pages:212-214 https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b kostenfrei http://www.scopemed.org/mnstemps/2/2-1296640457.pdf kostenfrei https://doaj.org/toc/0972-8988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2010 5.000 212-214
allfieldsSound	(DE-627)DOAJ067331467 (DE-599)DOAJ55d8bb49533f48489f1e81c4aadb443b DE-627 ger DE-627 rakwb eng SF1-1100 SF600-1100 A. Manicavasaka Dinakaran verfasserin aut Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214] Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine G. Selvaraju verfasserin aut K. Jayalakshmi verfasserin aut T.R. Gopalakrishna Murthy verfasserin aut M. Geetha and S. Saravanan verfasserin aut In Veterinary World Veterinary World, 2011 3(2010), 5.000, Seite 212-214 (DE-627)582020751 (DE-600)2456277-4 22310916 nnns volume:3 year:2010 number:5.000 pages:212-214 https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b kostenfrei http://www.scopemed.org/mnstemps/2/2-1296640457.pdf kostenfrei https://doaj.org/toc/0972-8988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 3 2010 5.000 212-214
language	English
source	In Veterinary World 3(2010), 5.000, Seite 212-214 volume:3 year:2010 number:5.000 pages:212-214
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topic_facet	Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test Animal culture Veterinary medicine
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authorswithroles_txt_mv	A. Manicavasaka Dinakaran @@aut@@ G. Selvaraju @@aut@@ K. Jayalakshmi @@aut@@ T.R. Gopalakrishna Murthy @@aut@@ M. Geetha and S. Saravanan @@aut@@
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callnumber-first	S - Agriculture
author	A. Manicavasaka Dinakaran
spellingShingle	A. Manicavasaka Dinakaran misc SF1-1100 misc SF600-1100 misc Marek’s disease misc Herpesvirus of turkey misc Polymerase chain reaction misc Agar gel immunodiffusion test misc Animal culture misc Veterinary medicine Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus
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topic_title	SF1-1100 SF600-1100 Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus Marek’s disease Herpesvirus of turkey Polymerase chain reaction Agar gel immunodiffusion test
topic	misc SF1-1100 misc SF600-1100 misc Marek’s disease misc Herpesvirus of turkey misc Polymerase chain reaction misc Agar gel immunodiffusion test misc Animal culture misc Veterinary medicine
topic_unstemmed	misc SF1-1100 misc SF600-1100 misc Marek’s disease misc Herpesvirus of turkey misc Polymerase chain reaction misc Agar gel immunodiffusion test misc Animal culture misc Veterinary medicine
topic_browse	misc SF1-1100 misc SF600-1100 misc Marek’s disease misc Herpesvirus of turkey misc Polymerase chain reaction misc Agar gel immunodiffusion test misc Animal culture misc Veterinary medicine
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title	Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus
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title_full	Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus
author_sort	A. Manicavasaka Dinakaran
journal	Veterinary World
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author_browse	A. Manicavasaka Dinakaran G. Selvaraju K. Jayalakshmi T.R. Gopalakrishna Murthy M. Geetha and S. Saravanan
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author-letter	A. Manicavasaka Dinakaran
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title_sort	comparision of polymerase chain reaction and agar gel immunodiffusion test in detection of marekand#8217;s disease virus
callnumber	SF1-1100
title_auth	Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus
abstract	A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214]
abstractGer	A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214]
abstract_unstemmed	A study was undertaken to identify Marek’s disease virus (MDV) antigen by PCR and AGID and to test the significance of PCR and AGID by McNemar’s test in detection of MDV antigen in outbreak in layer flocks. A total of twelve different MD outbreak flocks with varying flock size were selected in this study. Feather follicles were collected from 10 apparently healthy birds, 10 clinically affected birds and 10 dead birds separately in each outbreak. All the samples were subjected to PCR and AGID. In PCR, 42 (35.00%), 68 (56.67%) and 106 (88.33%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively and in AGID 28 (23.33%), 56 (46.67%) and 98 (81.67%) samples were positive to MDV in apparently healthy birds, clinically affected birds and dead birds respectively. In testing the significance of PCR and AGID in detecting MDV, significant difference existed between the two tests in feather tips of apparently healthy birds (P < 0.05), whereas there was no significant difference between PCR and AGID in detection of MDV in feather tips of clinically affected and dead birds (P > 0.05). Hence, PCR can be used to screen MDV in apparently healthy birds and AGID can be used to screen MDV in clinically affected and dead birds keeping feasibility and economic consideration. [Vet. World 2010; 3(5.000): 212-214]
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title_short	Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus
url	https://doaj.org/article/55d8bb49533f48489f1e81c4aadb443b http://www.scopemed.org/mnstemps/2/2-1296640457.pdf https://doaj.org/toc/0972-8988
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Comparision of Polymerase Chain Reaction and Agar Gel Immunodiffusion test in Detection of MarekAND#8217;s Disease Virus

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