Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<

<p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, r...
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Autor*in:	Wu Long-Fei [verfasserIn] Xu Jianguo [verfasserIn] Gao Shouyi [verfasserIn] Yan Meiying [verfasserIn] Xiong Yanwen [verfasserIn] Zhang Jingyun [verfasserIn] Jing Huaiqi [verfasserIn] Zhu Zhaoqin [verfasserIn] Zhang Lijuan [verfasserIn] Kan Biao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2009

Übergeordnetes Werk:	In: BMC Microbiology - BMC, 2003, 9(2009), 1, p 114
Übergeordnetes Werk:	volume:9 ; year:2009 ; number:1, p 114

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2180-9-114

Katalog-ID:	DOAJ067478255

Internformat


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allfields	10.1186/1471-2180-9-114 doi (DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92 DE-627 ger DE-627 rakwb eng QR1-502 Wu Long-Fei verfasserin aut Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it< 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p< Microbiology Xu Jianguo verfasserin aut Gao Shouyi verfasserin aut Yan Meiying verfasserin aut Xiong Yanwen verfasserin aut Zhang Jingyun verfasserin aut Jing Huaiqi verfasserin aut Zhu Zhaoqin verfasserin aut Zhang Lijuan verfasserin aut Kan Biao verfasserin aut In BMC Microbiology BMC, 2003 9(2009), 1, p 114 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:9 year:2009 number:1, p 114 https://doi.org/10.1186/1471-2180-9-114 kostenfrei https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 kostenfrei http://www.biomedcentral.com/1471-2180/9/114 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 114
spelling	10.1186/1471-2180-9-114 doi (DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92 DE-627 ger DE-627 rakwb eng QR1-502 Wu Long-Fei verfasserin aut Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it< 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p< Microbiology Xu Jianguo verfasserin aut Gao Shouyi verfasserin aut Yan Meiying verfasserin aut Xiong Yanwen verfasserin aut Zhang Jingyun verfasserin aut Jing Huaiqi verfasserin aut Zhu Zhaoqin verfasserin aut Zhang Lijuan verfasserin aut Kan Biao verfasserin aut In BMC Microbiology BMC, 2003 9(2009), 1, p 114 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:9 year:2009 number:1, p 114 https://doi.org/10.1186/1471-2180-9-114 kostenfrei https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 kostenfrei http://www.biomedcentral.com/1471-2180/9/114 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 114
allfields_unstemmed	10.1186/1471-2180-9-114 doi (DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92 DE-627 ger DE-627 rakwb eng QR1-502 Wu Long-Fei verfasserin aut Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it< 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p< Microbiology Xu Jianguo verfasserin aut Gao Shouyi verfasserin aut Yan Meiying verfasserin aut Xiong Yanwen verfasserin aut Zhang Jingyun verfasserin aut Jing Huaiqi verfasserin aut Zhu Zhaoqin verfasserin aut Zhang Lijuan verfasserin aut Kan Biao verfasserin aut In BMC Microbiology BMC, 2003 9(2009), 1, p 114 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:9 year:2009 number:1, p 114 https://doi.org/10.1186/1471-2180-9-114 kostenfrei https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 kostenfrei http://www.biomedcentral.com/1471-2180/9/114 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 114
allfieldsGer	10.1186/1471-2180-9-114 doi (DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92 DE-627 ger DE-627 rakwb eng QR1-502 Wu Long-Fei verfasserin aut Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it< 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p< Microbiology Xu Jianguo verfasserin aut Gao Shouyi verfasserin aut Yan Meiying verfasserin aut Xiong Yanwen verfasserin aut Zhang Jingyun verfasserin aut Jing Huaiqi verfasserin aut Zhu Zhaoqin verfasserin aut Zhang Lijuan verfasserin aut Kan Biao verfasserin aut In BMC Microbiology BMC, 2003 9(2009), 1, p 114 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:9 year:2009 number:1, p 114 https://doi.org/10.1186/1471-2180-9-114 kostenfrei https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 kostenfrei http://www.biomedcentral.com/1471-2180/9/114 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 114
allfieldsSound	10.1186/1471-2180-9-114 doi (DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92 DE-627 ger DE-627 rakwb eng QR1-502 Wu Long-Fei verfasserin aut Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it< 2009 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p< Microbiology Xu Jianguo verfasserin aut Gao Shouyi verfasserin aut Yan Meiying verfasserin aut Xiong Yanwen verfasserin aut Zhang Jingyun verfasserin aut Jing Huaiqi verfasserin aut Zhu Zhaoqin verfasserin aut Zhang Lijuan verfasserin aut Kan Biao verfasserin aut In BMC Microbiology BMC, 2003 9(2009), 1, p 114 (DE-627)326644997 (DE-600)2041505-9 14712180 nnns volume:9 year:2009 number:1, p 114 https://doi.org/10.1186/1471-2180-9-114 kostenfrei https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 kostenfrei http://www.biomedcentral.com/1471-2180/9/114 kostenfrei https://doaj.org/toc/1471-2180 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2009 1, p 114
language	English
source	In BMC Microbiology 9(2009), 1, p 114 volume:9 year:2009 number:1, p 114
sourceStr	In BMC Microbiology 9(2009), 1, p 114 volume:9 year:2009 number:1, p 114
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microbiology
isfreeaccess_bool	true
container_title	BMC Microbiology
authorswithroles_txt_mv	Wu Long-Fei @@aut@@ Xu Jianguo @@aut@@ Gao Shouyi @@aut@@ Yan Meiying @@aut@@ Xiong Yanwen @@aut@@ Zhang Jingyun @@aut@@ Jing Huaiqi @@aut@@ Zhu Zhaoqin @@aut@@ Zhang Lijuan @@aut@@ Kan Biao @@aut@@
publishDateDaySort_date	2009-01-01T00:00:00Z
hierarchy_top_id	326644997
id	DOAJ067478255
language_de	englisch
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callnumber-first	Q - Science
author	Wu Long-Fei
spellingShingle	Wu Long-Fei misc QR1-502 misc Microbiology Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
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topic_title	QR1-502 Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
topic	misc QR1-502 misc Microbiology
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title	Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
ctrlnum	(DE-627)DOAJ067478255 (DE-599)DOAJe91eca92ac864a3daf025f3f616c0d92
title_full	Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
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author_browse	Wu Long-Fei Xu Jianguo Gao Shouyi Yan Meiying Xiong Yanwen Zhang Jingyun Jing Huaiqi Zhu Zhaoqin Zhang Lijuan Kan Biao
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author-letter	Wu Long-Fei
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title_sort	pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<vibrio cholerae</it<
callnumber	QR1-502
title_auth	Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
abstract	<p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<The Twin-arginine translocation (Tat) system serves to translocate folded proteins, including periplasmic enzymes that bind redox cofactors in bacteria. The Tat system is also a determinant of virulence in some pathogenic bacteria, related to pleiotropic effects including growth, motility, and the secretion of some virulent factors. The contribution of the Tat pathway to <it<Vibrio cholerae </it<has not been explored. Here we investigated the functionality of the Tat system in <it<V. cholerae</it<, the etiologic agent of cholera.</p< <p<Results</p< <p<In <it<V. cholerae</it<, the <it<tatABC </it<genes function in the translocation of TMAO reductase. Deletion of the <it<tatABC </it<genes led to a significant decrease in biofilm formation, the ability to attach to HT-29 cells, and the ability to colonize suckling mouse intestines. In addition, we observed a reduction in the output of cholera toxin, which may be due to the decreased transcription level of the toxin gene in <it<tatABC </it<mutants, suggesting an indirect effect of the mutation on toxin production. No obvious differences in flagellum biosynthesis and motility were found between the <it<tatABC </it<mutant and the parental strain, showing a variable effect of Tat in different bacteria.</p< <p<Conclusion</p< <p<The Tat system contributes to the survival of <it<V. cholerae </it<in the environment and <it<in vivo</it<, and it may be associated with its virulence.</p<
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container_issue	1, p 114
title_short	Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<
url	https://doi.org/10.1186/1471-2180-9-114 https://doaj.org/article/e91eca92ac864a3daf025f3f616c0d92 http://www.biomedcentral.com/1471-2180/9/114 https://doaj.org/toc/1471-2180
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Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in <it<Vibrio cholerae</it<

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