Banking of AT-MSC and its Influence on Their Application to Clinical Procedures
Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells re...
Ausführliche Beschreibung
Autor*in: |
Ekaterina Semenova [verfasserIn] Mariusz P. Grudniak [verfasserIn] Katarzyna Bocian [verfasserIn] Magdalena Chroscinska-Krawczyk [verfasserIn] Marzena Trochonowicz [verfasserIn] Igor M. Stepaniec [verfasserIn] Magdalena Murzyn [verfasserIn] Ilona Szablowska-Gadomska [verfasserIn] Dariusz Boruczkowski [verfasserIn] Tomasz Oldak [verfasserIn] Eugeniusz K. Machaj [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2021 |
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Übergeordnetes Werk: |
In: Frontiers in Bioengineering and Biotechnology - Frontiers Media S.A., 2014, 9(2021) |
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Übergeordnetes Werk: |
volume:9 ; year:2021 |
Links: |
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DOI / URN: |
10.3389/fbioe.2021.773123 |
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Katalog-ID: |
DOAJ067744001 |
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10.3389/fbioe.2021.773123 doi (DE-627)DOAJ067744001 (DE-599)DOAJ69fcf72aa9d34a73b3812723737a733f DE-627 ger DE-627 rakwb eng TP248.13-248.65 Ekaterina Semenova verfasserin aut Banking of AT-MSC and its Influence on Their Application to Clinical Procedures 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. cryopreservation banking cryoprotectant ADSC application Biotechnology Mariusz P. Grudniak verfasserin aut Katarzyna Bocian verfasserin aut Katarzyna Bocian verfasserin aut Magdalena Chroscinska-Krawczyk verfasserin aut Marzena Trochonowicz verfasserin aut Igor M. Stepaniec verfasserin aut Magdalena Murzyn verfasserin aut Magdalena Murzyn verfasserin aut Ilona Szablowska-Gadomska verfasserin aut Dariusz Boruczkowski verfasserin aut Tomasz Oldak verfasserin aut Eugeniusz K. Machaj verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2021) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2021 https://doi.org/10.3389/fbioe.2021.773123 kostenfrei https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 |
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10.3389/fbioe.2021.773123 doi (DE-627)DOAJ067744001 (DE-599)DOAJ69fcf72aa9d34a73b3812723737a733f DE-627 ger DE-627 rakwb eng TP248.13-248.65 Ekaterina Semenova verfasserin aut Banking of AT-MSC and its Influence on Their Application to Clinical Procedures 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. cryopreservation banking cryoprotectant ADSC application Biotechnology Mariusz P. Grudniak verfasserin aut Katarzyna Bocian verfasserin aut Katarzyna Bocian verfasserin aut Magdalena Chroscinska-Krawczyk verfasserin aut Marzena Trochonowicz verfasserin aut Igor M. Stepaniec verfasserin aut Magdalena Murzyn verfasserin aut Magdalena Murzyn verfasserin aut Ilona Szablowska-Gadomska verfasserin aut Dariusz Boruczkowski verfasserin aut Tomasz Oldak verfasserin aut Eugeniusz K. Machaj verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2021) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2021 https://doi.org/10.3389/fbioe.2021.773123 kostenfrei https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 |
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10.3389/fbioe.2021.773123 doi (DE-627)DOAJ067744001 (DE-599)DOAJ69fcf72aa9d34a73b3812723737a733f DE-627 ger DE-627 rakwb eng TP248.13-248.65 Ekaterina Semenova verfasserin aut Banking of AT-MSC and its Influence on Their Application to Clinical Procedures 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. cryopreservation banking cryoprotectant ADSC application Biotechnology Mariusz P. Grudniak verfasserin aut Katarzyna Bocian verfasserin aut Katarzyna Bocian verfasserin aut Magdalena Chroscinska-Krawczyk verfasserin aut Marzena Trochonowicz verfasserin aut Igor M. Stepaniec verfasserin aut Magdalena Murzyn verfasserin aut Magdalena Murzyn verfasserin aut Ilona Szablowska-Gadomska verfasserin aut Dariusz Boruczkowski verfasserin aut Tomasz Oldak verfasserin aut Eugeniusz K. Machaj verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2021) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2021 https://doi.org/10.3389/fbioe.2021.773123 kostenfrei https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 |
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10.3389/fbioe.2021.773123 doi (DE-627)DOAJ067744001 (DE-599)DOAJ69fcf72aa9d34a73b3812723737a733f DE-627 ger DE-627 rakwb eng TP248.13-248.65 Ekaterina Semenova verfasserin aut Banking of AT-MSC and its Influence on Their Application to Clinical Procedures 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. cryopreservation banking cryoprotectant ADSC application Biotechnology Mariusz P. Grudniak verfasserin aut Katarzyna Bocian verfasserin aut Katarzyna Bocian verfasserin aut Magdalena Chroscinska-Krawczyk verfasserin aut Marzena Trochonowicz verfasserin aut Igor M. Stepaniec verfasserin aut Magdalena Murzyn verfasserin aut Magdalena Murzyn verfasserin aut Ilona Szablowska-Gadomska verfasserin aut Dariusz Boruczkowski verfasserin aut Tomasz Oldak verfasserin aut Eugeniusz K. Machaj verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2021) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2021 https://doi.org/10.3389/fbioe.2021.773123 kostenfrei https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 |
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10.3389/fbioe.2021.773123 doi (DE-627)DOAJ067744001 (DE-599)DOAJ69fcf72aa9d34a73b3812723737a733f DE-627 ger DE-627 rakwb eng TP248.13-248.65 Ekaterina Semenova verfasserin aut Banking of AT-MSC and its Influence on Their Application to Clinical Procedures 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. cryopreservation banking cryoprotectant ADSC application Biotechnology Mariusz P. Grudniak verfasserin aut Katarzyna Bocian verfasserin aut Katarzyna Bocian verfasserin aut Magdalena Chroscinska-Krawczyk verfasserin aut Marzena Trochonowicz verfasserin aut Igor M. Stepaniec verfasserin aut Magdalena Murzyn verfasserin aut Magdalena Murzyn verfasserin aut Ilona Szablowska-Gadomska verfasserin aut Dariusz Boruczkowski verfasserin aut Tomasz Oldak verfasserin aut Eugeniusz K. Machaj verfasserin aut In Frontiers in Bioengineering and Biotechnology Frontiers Media S.A., 2014 9(2021) (DE-627)74950403X (DE-600)2719493-0 22964185 nnns volume:9 year:2021 https://doi.org/10.3389/fbioe.2021.773123 kostenfrei https://doaj.org/article/69fcf72aa9d34a73b3812723737a733f kostenfrei https://www.frontiersin.org/articles/10.3389/fbioe.2021.773123/full kostenfrei https://doaj.org/toc/2296-4185 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 |
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Banking of AT-MSC and its Influence on Their Application to Clinical Procedures |
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Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. |
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Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. |
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Processing of MSCs to obtain a therapeutic product consists of two main steps: 1) the in vitro expansion of the cells until an appropriate number of them is obtained, and 2) freezing and storage of the expanded cells. The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. We suggest that before their clinical application the cells should be cultured for at least one passage to recover their physiological stability and thus assure their optimal therapeutic potential. |
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The last step is critical and must be optimized so that after thawing the cells retain all their physiological properties including the secretory function. In this paper, we evaluated physiological parameters of AT-MSC’s after a full cycle of their processing, particularly freezing and storing at the liquid nitrogen vapor temperature. Based on the recovered proliferative and secretory capacities of the thawed cells, we have designed the optimal technique for processing of MSCs for clinical applications. In our work, we tried to select the best DMSO-based cryoprotectant mixture on the base of post thawing fully retain their properties. We have demonstrated the effectiveness of the use of DMSO in various configurations of the constituent cryoprotective fluids. We have also shown that AT-MSCs that show control levels in most standard tests (viability, shape, culture behaviour, and proliferative properties) after thawing, may show transient variations in some important physiological properties, such as the level of secreted growth factors. Obtained results let us to indicate how to optimize the AT-MSC preparation process for clinical applications. 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