In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and...
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Autor*in:	Tetsuya Adachi [verfasserIn] Francesco Boschetto [verfasserIn] Nao Miyamoto [verfasserIn] Toshiro Yamamoto [verfasserIn] Elia Marin [verfasserIn] Wenliang Zhu [verfasserIn] Narisato Kanamura [verfasserIn] Yoshiro Tahara [verfasserIn] Kazunari Akiyoshi [verfasserIn] Osam Mazda [verfasserIn] Ichiro Nishimura [verfasserIn] Giuseppe Pezzotti [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy

Übergeordnetes Werk:	In: Materials - MDPI AG, 2009, 13(2020), 19, p 4275
Übergeordnetes Werk:	volume:13 ; year:2020 ; number:19, p 4275

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ma13194275

Katalog-ID:	DOAJ067747256

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allfields	10.3390/ma13194275 doi (DE-627)DOAJ067747256 (DE-599)DOAJc0237079b1e14c13bbfac917c5c41434 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Tetsuya Adachi verfasserin aut In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Francesco Boschetto verfasserin aut Nao Miyamoto verfasserin aut Toshiro Yamamoto verfasserin aut Elia Marin verfasserin aut Wenliang Zhu verfasserin aut Narisato Kanamura verfasserin aut Yoshiro Tahara verfasserin aut Kazunari Akiyoshi verfasserin aut Osam Mazda verfasserin aut Ichiro Nishimura verfasserin aut Giuseppe Pezzotti verfasserin aut In Materials MDPI AG, 2009 13(2020), 19, p 4275 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:13 year:2020 number:19, p 4275 https://doi.org/10.3390/ma13194275 kostenfrei https://doaj.org/article/c0237079b1e14c13bbfac917c5c41434 kostenfrei https://www.mdpi.com/1996-1944/13/19/4275 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2020 19, p 4275
spelling	10.3390/ma13194275 doi (DE-627)DOAJ067747256 (DE-599)DOAJc0237079b1e14c13bbfac917c5c41434 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Tetsuya Adachi verfasserin aut In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Francesco Boschetto verfasserin aut Nao Miyamoto verfasserin aut Toshiro Yamamoto verfasserin aut Elia Marin verfasserin aut Wenliang Zhu verfasserin aut Narisato Kanamura verfasserin aut Yoshiro Tahara verfasserin aut Kazunari Akiyoshi verfasserin aut Osam Mazda verfasserin aut Ichiro Nishimura verfasserin aut Giuseppe Pezzotti verfasserin aut In Materials MDPI AG, 2009 13(2020), 19, p 4275 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:13 year:2020 number:19, p 4275 https://doi.org/10.3390/ma13194275 kostenfrei https://doaj.org/article/c0237079b1e14c13bbfac917c5c41434 kostenfrei https://www.mdpi.com/1996-1944/13/19/4275 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2020 19, p 4275
allfields_unstemmed	10.3390/ma13194275 doi (DE-627)DOAJ067747256 (DE-599)DOAJc0237079b1e14c13bbfac917c5c41434 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Tetsuya Adachi verfasserin aut In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Francesco Boschetto verfasserin aut Nao Miyamoto verfasserin aut Toshiro Yamamoto verfasserin aut Elia Marin verfasserin aut Wenliang Zhu verfasserin aut Narisato Kanamura verfasserin aut Yoshiro Tahara verfasserin aut Kazunari Akiyoshi verfasserin aut Osam Mazda verfasserin aut Ichiro Nishimura verfasserin aut Giuseppe Pezzotti verfasserin aut In Materials MDPI AG, 2009 13(2020), 19, p 4275 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:13 year:2020 number:19, p 4275 https://doi.org/10.3390/ma13194275 kostenfrei https://doaj.org/article/c0237079b1e14c13bbfac917c5c41434 kostenfrei https://www.mdpi.com/1996-1944/13/19/4275 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2020 19, p 4275
allfieldsGer	10.3390/ma13194275 doi (DE-627)DOAJ067747256 (DE-599)DOAJc0237079b1e14c13bbfac917c5c41434 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Tetsuya Adachi verfasserin aut In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Francesco Boschetto verfasserin aut Nao Miyamoto verfasserin aut Toshiro Yamamoto verfasserin aut Elia Marin verfasserin aut Wenliang Zhu verfasserin aut Narisato Kanamura verfasserin aut Yoshiro Tahara verfasserin aut Kazunari Akiyoshi verfasserin aut Osam Mazda verfasserin aut Ichiro Nishimura verfasserin aut Giuseppe Pezzotti verfasserin aut In Materials MDPI AG, 2009 13(2020), 19, p 4275 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:13 year:2020 number:19, p 4275 https://doi.org/10.3390/ma13194275 kostenfrei https://doaj.org/article/c0237079b1e14c13bbfac917c5c41434 kostenfrei https://www.mdpi.com/1996-1944/13/19/4275 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2020 19, p 4275
allfieldsSound	10.3390/ma13194275 doi (DE-627)DOAJ067747256 (DE-599)DOAJc0237079b1e14c13bbfac917c5c41434 DE-627 ger DE-627 rakwb eng TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 Tetsuya Adachi verfasserin aut In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects. spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics Francesco Boschetto verfasserin aut Nao Miyamoto verfasserin aut Toshiro Yamamoto verfasserin aut Elia Marin verfasserin aut Wenliang Zhu verfasserin aut Narisato Kanamura verfasserin aut Yoshiro Tahara verfasserin aut Kazunari Akiyoshi verfasserin aut Osam Mazda verfasserin aut Ichiro Nishimura verfasserin aut Giuseppe Pezzotti verfasserin aut In Materials MDPI AG, 2009 13(2020), 19, p 4275 (DE-627)595712649 (DE-600)2487261-1 19961944 nnns volume:13 year:2020 number:19, p 4275 https://doi.org/10.3390/ma13194275 kostenfrei https://doaj.org/article/c0237079b1e14c13bbfac917c5c41434 kostenfrei https://www.mdpi.com/1996-1944/13/19/4275 kostenfrei https://doaj.org/toc/1996-1944 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2057 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2020 19, p 4275
language	English
source	In Materials 13(2020), 19, p 4275 volume:13 year:2020 number:19, p 4275
sourceStr	In Materials 13(2020), 19, p 4275 volume:13 year:2020 number:19, p 4275
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy Technology T Electrical engineering. Electronics. Nuclear engineering Engineering (General). Civil engineering (General) Microscopy Descriptive and experimental mechanics
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container_title	Materials
authorswithroles_txt_mv	Tetsuya Adachi @@aut@@ Francesco Boschetto @@aut@@ Nao Miyamoto @@aut@@ Toshiro Yamamoto @@aut@@ Elia Marin @@aut@@ Wenliang Zhu @@aut@@ Narisato Kanamura @@aut@@ Yoshiro Tahara @@aut@@ Kazunari Akiyoshi @@aut@@ Osam Mazda @@aut@@ Ichiro Nishimura @@aut@@ Giuseppe Pezzotti @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	595712649
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callnumber-first	T - Technology
author	Tetsuya Adachi
spellingShingle	Tetsuya Adachi misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc spectroscopic imaging misc nanogel misc scaffold misc bone tissue regeneration misc Raman spectroscopy misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
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topic_title	TK1-9971 TA1-2040 QH201-278.5 QC120-168.85 In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging spectroscopic imaging nanogel scaffold bone tissue regeneration Raman spectroscopy
topic	misc TK1-9971 misc TA1-2040 misc QH201-278.5 misc QC120-168.85 misc spectroscopic imaging misc nanogel misc scaffold misc bone tissue regeneration misc Raman spectroscopy misc Technology misc T misc Electrical engineering. Electronics. Nuclear engineering misc Engineering (General). Civil engineering (General) misc Microscopy misc Descriptive and experimental mechanics
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title	In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
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title_full	In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
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author_browse	Tetsuya Adachi Francesco Boschetto Nao Miyamoto Toshiro Yamamoto Elia Marin Wenliang Zhu Narisato Kanamura Yoshiro Tahara Kazunari Akiyoshi Osam Mazda Ichiro Nishimura Giuseppe Pezzotti
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title_sort	in vivo regeneration of large bone defects by cross-linked porous hydrogel: a pilot study in mice combining micro tomography, histological analyses, raman spectroscopy and synchrotron infrared imaging
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title_auth	In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
abstract	The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.
abstractGer	The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.
abstract_unstemmed	The transplantation of engineered three-dimensional (3D) bone graft substitutes is a viable approach to the regeneration of severe bone defects. For large bone defects, an appropriate 3D scaffold may be necessary to support and stimulate bone regeneration, even when a sufficient number of cells and cell cytokines are available. In this study, we evaluated the in vivo performance of a nanogel tectonic 3D scaffold specifically developed for bone tissue engineering, referred to as nanogel cross-linked porous-freeze-dry (NanoCliP-FD) gel. Samples were characterized by a combination of micro-computed tomography scanning, Raman spectroscopy, histological analyses, and synchrotron radiation–based Fourier transform infrared spectroscopy. NanoCliP-FD gel is a modified version of a previously developed nanogel cross-linked porous (NanoCliP) gel and was designed to achieve highly improved functionality in bone mineralization. Spectroscopic imaging of the bone tissue grown in vivo upon application of NanoCliP-FD gel enables an evaluation of bone quality and can be employed to judge the feasibility of NanoCliP-FD gel scaffolding as a therapeutic modality for bone diseases associated with large bone defects.
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title_short	In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging
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In Vivo Regeneration of Large Bone Defects by Cross-Linked Porous Hydrogel: A Pilot Study in Mice Combining Micro Tomography, Histological Analyses, Raman Spectroscopy and Synchrotron Infrared Imaging

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