Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts
The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Anja Smits [verfasserIn] Per Odin [verfasserIn] Wei-Ming Duan [verfasserIn] Patrik Brundin [verfasserIn] Håkan Widner [verfasserIn] Carl-Henrik Heldin [verfasserIn] Olle Lindvall [verfasserIn] Keiko Funa [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1993 |
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| Übergeordnetes Werk: |
In: Cell Transplantation - SAGE Publishing, 2017, 2(1993) |
|---|---|
| Übergeordnetes Werk: |
volume:2 ; year:1993 |
| Links: |
Link aufrufen |
|---|
| DOI / URN: |
10.1177/096368979300200208 |
|---|
| Katalog-ID: |
DOAJ067800041 |
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| 520 | |a The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. | ||
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10.1177/096368979300200208 doi (DE-627)DOAJ067800041 (DE-599)DOAJ10d7e5337f8944539e1443b716c39c0e DE-627 ger DE-627 rakwb eng Anja Smits verfasserin aut Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts 1993 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. Medicine R Per Odin verfasserin aut Wei-Ming Duan verfasserin aut Patrik Brundin verfasserin aut Håkan Widner verfasserin aut Carl-Henrik Heldin verfasserin aut Olle Lindvall verfasserin aut Keiko Funa verfasserin aut In Cell Transplantation SAGE Publishing, 2017 2(1993) (DE-627)320603504 (DE-600)2020466-8 15553892 nnns volume:2 year:1993 https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e kostenfrei https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/toc/0963-6897 Journal toc kostenfrei https://doaj.org/toc/1555-3892 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_4325 AR 2 1993 |
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10.1177/096368979300200208 doi (DE-627)DOAJ067800041 (DE-599)DOAJ10d7e5337f8944539e1443b716c39c0e DE-627 ger DE-627 rakwb eng Anja Smits verfasserin aut Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts 1993 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. Medicine R Per Odin verfasserin aut Wei-Ming Duan verfasserin aut Patrik Brundin verfasserin aut Håkan Widner verfasserin aut Carl-Henrik Heldin verfasserin aut Olle Lindvall verfasserin aut Keiko Funa verfasserin aut In Cell Transplantation SAGE Publishing, 2017 2(1993) (DE-627)320603504 (DE-600)2020466-8 15553892 nnns volume:2 year:1993 https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e kostenfrei https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/toc/0963-6897 Journal toc kostenfrei https://doaj.org/toc/1555-3892 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_4325 AR 2 1993 |
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10.1177/096368979300200208 doi (DE-627)DOAJ067800041 (DE-599)DOAJ10d7e5337f8944539e1443b716c39c0e DE-627 ger DE-627 rakwb eng Anja Smits verfasserin aut Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts 1993 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. Medicine R Per Odin verfasserin aut Wei-Ming Duan verfasserin aut Patrik Brundin verfasserin aut Håkan Widner verfasserin aut Carl-Henrik Heldin verfasserin aut Olle Lindvall verfasserin aut Keiko Funa verfasserin aut In Cell Transplantation SAGE Publishing, 2017 2(1993) (DE-627)320603504 (DE-600)2020466-8 15553892 nnns volume:2 year:1993 https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e kostenfrei https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/toc/0963-6897 Journal toc kostenfrei https://doaj.org/toc/1555-3892 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_4325 AR 2 1993 |
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10.1177/096368979300200208 doi (DE-627)DOAJ067800041 (DE-599)DOAJ10d7e5337f8944539e1443b716c39c0e DE-627 ger DE-627 rakwb eng Anja Smits verfasserin aut Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts 1993 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. Medicine R Per Odin verfasserin aut Wei-Ming Duan verfasserin aut Patrik Brundin verfasserin aut Håkan Widner verfasserin aut Carl-Henrik Heldin verfasserin aut Olle Lindvall verfasserin aut Keiko Funa verfasserin aut In Cell Transplantation SAGE Publishing, 2017 2(1993) (DE-627)320603504 (DE-600)2020466-8 15553892 nnns volume:2 year:1993 https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e kostenfrei https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/toc/0963-6897 Journal toc kostenfrei https://doaj.org/toc/1555-3892 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_4325 AR 2 1993 |
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10.1177/096368979300200208 doi (DE-627)DOAJ067800041 (DE-599)DOAJ10d7e5337f8944539e1443b716c39c0e DE-627 ger DE-627 rakwb eng Anja Smits verfasserin aut Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts 1993 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. Medicine R Per Odin verfasserin aut Wei-Ming Duan verfasserin aut Patrik Brundin verfasserin aut Håkan Widner verfasserin aut Carl-Henrik Heldin verfasserin aut Olle Lindvall verfasserin aut Keiko Funa verfasserin aut In Cell Transplantation SAGE Publishing, 2017 2(1993) (DE-627)320603504 (DE-600)2020466-8 15553892 nnns volume:2 year:1993 https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e kostenfrei https://doi.org/10.1177/096368979300200208 kostenfrei https://doaj.org/toc/0963-6897 Journal toc kostenfrei https://doaj.org/toc/1555-3892 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_4325 AR 2 1993 |
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Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts |
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Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts |
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Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts |
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Anja Smits |
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Cell Transplantation |
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1993 |
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Anja Smits Per Odin Wei-Ming Duan Patrik Brundin Håkan Widner Carl-Henrik Heldin Olle Lindvall Keiko Funa |
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expression of platelet-derived growth factor in and around intrastriatal embryonic mesencephalic grafts |
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Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts |
| abstract |
The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. |
| abstractGer |
The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. |
| abstract_unstemmed |
The expression of platelet-derived growth factor (PDGF) was investigated in the embryonic donor tissue and surrounding host brain before and after intracerebral transplantation in a rat model of Parkinson's disease (PD). Ventral mesencephalic tissue from E13-E15 rat embryos was dissociated and implanted into adult rats with unilateral lesions of the mesostriatal dopamine system. Immunohistochemical studies showed that the majority of the grafted cells were PDGF-positive at early time points after grafting. However, the immunostaining gradually decreased, and had disappeared almost completely 3 wk after transplantation. These results were in agreement with in situ hybridization data demonstrating detectable levels of mRNA for PDGF chains in graft cells after 1, but not after 6 wk. In contrast, a large number of PDGF-immunoreactive cells was observed in the host brain adjacent to the grafts from 1 wk after transplantation, and increasing with time. Increased expression of PDGF was also observed in response to a sham-operation (injection of vehicle), although the number of PDGF-positive cells seemed lower than after grafting of embryonic tissue. Double immunofluorescence labeling of these cells with an anti-glial fibrillary acidic protein (GFAP) antiserum and a monoclonal antibody against PDGF B-chain, indicated that the PDGF-positive cells were astrocytes. The dynamic expression of PDGF in and around intrastriatal embryonic mesencephalic implants has several, potentially important, implications for graft survival and function. Glial cells could utilize the elevated levels of PDGF to proliferate in a reactive gliosis, and PDGF might also augment immune responses. It is also possible that PDGF increases the survival of, and promotes neurite outgrowth from, grafted dopaminergic neurons. |
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Expression of Platelet-Derived Growth Factor in and around Intrastriatal Embryonic Mesencephalic Grafts |
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https://doi.org/10.1177/096368979300200208 https://doaj.org/article/10d7e5337f8944539e1443b716c39c0e https://doaj.org/toc/0963-6897 https://doaj.org/toc/1555-3892 |
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