Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)

The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Gianluca Neglia [verfasserIn] Marcello Rubessa [verfasserIn] Salvatore Velotto [verfasserIn] Serena Di Francesco [verfasserIn] Evelina Mariotti [verfasserIn] Luigi Zicarelli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Schlagwörter:	Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum.

Übergeordnetes Werk:	In: Italian Journal of Animal Science - Taylor & Francis Group, 2007, 8(2010), 2s, Seite 177-179
Übergeordnetes Werk:	volume:8 ; year:2010 ; number:2s ; pages:177-179

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.4081/ijas.2009.s2.177

Katalog-ID:	DOAJ068128843

Internformat


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allfields	10.4081/ijas.2009.s2.177 doi (DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d DE-627 ger DE-627 rakwb eng SF1-1100 Gianluca Neglia verfasserin aut Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively). Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. Animal culture Marcello Rubessa verfasserin aut Salvatore Velotto verfasserin aut Serena Di Francesco verfasserin aut Evelina Mariotti verfasserin aut Luigi Zicarelli verfasserin aut In Italian Journal of Animal Science Taylor & Francis Group, 2007 8(2010), 2s, Seite 177-179 (DE-627)558387608 (DE-600)2408994-1 1828051X nnns volume:8 year:2010 number:2s pages:177-179 https://doi.org/10.4081/ijas.2009.s2.177 kostenfrei https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d kostenfrei http://www.aspajournal.it/index.php/ijas/article/view/361 kostenfrei https://doaj.org/toc/1594-4077 Journal toc kostenfrei https://doaj.org/toc/1828-051X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 2s 177-179
spelling	10.4081/ijas.2009.s2.177 doi (DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d DE-627 ger DE-627 rakwb eng SF1-1100 Gianluca Neglia verfasserin aut Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively). Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. Animal culture Marcello Rubessa verfasserin aut Salvatore Velotto verfasserin aut Serena Di Francesco verfasserin aut Evelina Mariotti verfasserin aut Luigi Zicarelli verfasserin aut In Italian Journal of Animal Science Taylor & Francis Group, 2007 8(2010), 2s, Seite 177-179 (DE-627)558387608 (DE-600)2408994-1 1828051X nnns volume:8 year:2010 number:2s pages:177-179 https://doi.org/10.4081/ijas.2009.s2.177 kostenfrei https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d kostenfrei http://www.aspajournal.it/index.php/ijas/article/view/361 kostenfrei https://doaj.org/toc/1594-4077 Journal toc kostenfrei https://doaj.org/toc/1828-051X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 2s 177-179
allfields_unstemmed	10.4081/ijas.2009.s2.177 doi (DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d DE-627 ger DE-627 rakwb eng SF1-1100 Gianluca Neglia verfasserin aut Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively). Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. Animal culture Marcello Rubessa verfasserin aut Salvatore Velotto verfasserin aut Serena Di Francesco verfasserin aut Evelina Mariotti verfasserin aut Luigi Zicarelli verfasserin aut In Italian Journal of Animal Science Taylor & Francis Group, 2007 8(2010), 2s, Seite 177-179 (DE-627)558387608 (DE-600)2408994-1 1828051X nnns volume:8 year:2010 number:2s pages:177-179 https://doi.org/10.4081/ijas.2009.s2.177 kostenfrei https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d kostenfrei http://www.aspajournal.it/index.php/ijas/article/view/361 kostenfrei https://doaj.org/toc/1594-4077 Journal toc kostenfrei https://doaj.org/toc/1828-051X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 2s 177-179
allfieldsGer	10.4081/ijas.2009.s2.177 doi (DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d DE-627 ger DE-627 rakwb eng SF1-1100 Gianluca Neglia verfasserin aut Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively). Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. Animal culture Marcello Rubessa verfasserin aut Salvatore Velotto verfasserin aut Serena Di Francesco verfasserin aut Evelina Mariotti verfasserin aut Luigi Zicarelli verfasserin aut In Italian Journal of Animal Science Taylor & Francis Group, 2007 8(2010), 2s, Seite 177-179 (DE-627)558387608 (DE-600)2408994-1 1828051X nnns volume:8 year:2010 number:2s pages:177-179 https://doi.org/10.4081/ijas.2009.s2.177 kostenfrei https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d kostenfrei http://www.aspajournal.it/index.php/ijas/article/view/361 kostenfrei https://doaj.org/toc/1594-4077 Journal toc kostenfrei https://doaj.org/toc/1828-051X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 2s 177-179
allfieldsSound	10.4081/ijas.2009.s2.177 doi (DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d DE-627 ger DE-627 rakwb eng SF1-1100 Gianluca Neglia verfasserin aut Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively). Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. Animal culture Marcello Rubessa verfasserin aut Salvatore Velotto verfasserin aut Serena Di Francesco verfasserin aut Evelina Mariotti verfasserin aut Luigi Zicarelli verfasserin aut In Italian Journal of Animal Science Taylor & Francis Group, 2007 8(2010), 2s, Seite 177-179 (DE-627)558387608 (DE-600)2408994-1 1828051X nnns volume:8 year:2010 number:2s pages:177-179 https://doi.org/10.4081/ijas.2009.s2.177 kostenfrei https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d kostenfrei http://www.aspajournal.it/index.php/ijas/article/view/361 kostenfrei https://doaj.org/toc/1594-4077 Journal toc kostenfrei https://doaj.org/toc/1828-051X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2010 2s 177-179
language	English
source	In Italian Journal of Animal Science 8(2010), 2s, Seite 177-179 volume:8 year:2010 number:2s pages:177-179
sourceStr	In Italian Journal of Animal Science 8(2010), 2s, Seite 177-179 volume:8 year:2010 number:2s pages:177-179
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authorswithroles_txt_mv	Gianluca Neglia @@aut@@ Marcello Rubessa @@aut@@ Salvatore Velotto @@aut@@ Serena Di Francesco @@aut@@ Evelina Mariotti @@aut@@ Luigi Zicarelli @@aut@@
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callnumber-first	S - Agriculture
author	Gianluca Neglia
spellingShingle	Gianluca Neglia misc SF1-1100 misc Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. misc Animal culture Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)
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topic_title	SF1-1100 Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis) Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum
topic	misc SF1-1100 misc Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. misc Animal culture
topic_unstemmed	misc SF1-1100 misc Buffalo, Sperm capacitation, Heparin, Buffalo estrus serum. misc Animal culture
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title	Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)
ctrlnum	(DE-627)DOAJ068128843 (DE-599)DOAJb6927c3861fe4ad08eafde969db9745d
title_full	Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)
author_sort	Gianluca Neglia
journal	Italian Journal of Animal Science
journalStr	Italian Journal of Animal Science
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author_browse	Gianluca Neglia Marcello Rubessa Salvatore Velotto Serena Di Francesco Evelina Mariotti Luigi Zicarelli
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author-letter	Gianluca Neglia
doi_str_mv	10.4081/ijas.2009.s2.177
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title_sort	effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (bubalus bubalis)
callnumber	SF1-1100
title_auth	Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)
abstract	The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively).
abstractGer	The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively).
abstract_unstemmed	The aim of this work was to evaluate the effect of bull on the efficacy of different capacitating agents in buffalo. Spermatozoa derived from 4 different bulls were incubated in absence of a capacitating agent, in presence of 0.01 mM heparin and in 20% buffalo estrous serum (BES) for 2 hours. Sperm were then exposed to lysophosphatidylcholine, an agent that induces acrosome reaction only on capacitated spermatozoa. A double staining technique with Trypan-blue/Giemsa was used to evaluate viability and acrosome status of spermatozoa fixed in 37% formaldehyde. The efficiency of capacitation was evaluated by assessing the percentage of acrosome-reacted (AR) spermatozoa in the treated groups. A bull effect on sperm capacitation in vitro was demonstrated, as indicated by differences in the percentage of AR sperm among bulls regardless of the treatment. In particular when heparin was used as capacitating agent bull C gave higher percentages of AR sperm than bull A (22.7 vs. 14.0%, respectively) with intermediate values for bulls B and D (16.7 and 19.1%, respectively), whereas when BES was used bull D was the most efficient one (22.1, 21.9, 25.0, and 35.0% for bulls A, B, C and D, respectively).
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title_short	Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)
url	https://doi.org/10.4081/ijas.2009.s2.177 https://doaj.org/article/b6927c3861fe4ad08eafde969db9745d http://www.aspajournal.it/index.php/ijas/article/view/361 https://doaj.org/toc/1594-4077 https://doaj.org/toc/1828-051X
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author2	Marcello Rubessa Salvatore Velotto Serena Di Francesco Evelina Mariotti Luigi Zicarelli
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up_date	2024-07-03T16:02:29.352Z
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Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)

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