Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems

Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Federico Martinez-Seidel [verfasserIn] Pipob Suwanchaikasem [verfasserIn] Shuai Nie [verfasserIn] Michael G. Leeming [verfasserIn] Alexandre Augusto Pereira Firmino [verfasserIn] Nicholas A. Williamson [verfasserIn] Joachim Kopka [verfasserIn] Ute Roessner [verfasserIn] Berin A. Boughton [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 12(2021)
Übergeordnetes Werk:	volume:12 ; year:2021

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2021.656683

Katalog-ID:	DOAJ068284691

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allfields	10.3389/fpls.2021.656683 doi (DE-627)DOAJ068284691 (DE-599)DOAJf87cf1cdfaea410c9ad5d579acff199f DE-627 ger DE-627 rakwb eng SB1-1110 Federico Martinez-Seidel verfasserin aut Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture Federico Martinez-Seidel verfasserin aut Pipob Suwanchaikasem verfasserin aut Shuai Nie verfasserin aut Michael G. Leeming verfasserin aut Michael G. Leeming verfasserin aut Alexandre Augusto Pereira Firmino verfasserin aut Nicholas A. Williamson verfasserin aut Nicholas A. Williamson verfasserin aut Joachim Kopka verfasserin aut Ute Roessner verfasserin aut Berin A. Boughton verfasserin aut Berin A. Boughton verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.656683 kostenfrei https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
spelling	10.3389/fpls.2021.656683 doi (DE-627)DOAJ068284691 (DE-599)DOAJf87cf1cdfaea410c9ad5d579acff199f DE-627 ger DE-627 rakwb eng SB1-1110 Federico Martinez-Seidel verfasserin aut Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture Federico Martinez-Seidel verfasserin aut Pipob Suwanchaikasem verfasserin aut Shuai Nie verfasserin aut Michael G. Leeming verfasserin aut Michael G. Leeming verfasserin aut Alexandre Augusto Pereira Firmino verfasserin aut Nicholas A. Williamson verfasserin aut Nicholas A. Williamson verfasserin aut Joachim Kopka verfasserin aut Ute Roessner verfasserin aut Berin A. Boughton verfasserin aut Berin A. Boughton verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.656683 kostenfrei https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfields_unstemmed	10.3389/fpls.2021.656683 doi (DE-627)DOAJ068284691 (DE-599)DOAJf87cf1cdfaea410c9ad5d579acff199f DE-627 ger DE-627 rakwb eng SB1-1110 Federico Martinez-Seidel verfasserin aut Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture Federico Martinez-Seidel verfasserin aut Pipob Suwanchaikasem verfasserin aut Shuai Nie verfasserin aut Michael G. Leeming verfasserin aut Michael G. Leeming verfasserin aut Alexandre Augusto Pereira Firmino verfasserin aut Nicholas A. Williamson verfasserin aut Nicholas A. Williamson verfasserin aut Joachim Kopka verfasserin aut Ute Roessner verfasserin aut Berin A. Boughton verfasserin aut Berin A. Boughton verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.656683 kostenfrei https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsGer	10.3389/fpls.2021.656683 doi (DE-627)DOAJ068284691 (DE-599)DOAJf87cf1cdfaea410c9ad5d579acff199f DE-627 ger DE-627 rakwb eng SB1-1110 Federico Martinez-Seidel verfasserin aut Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture Federico Martinez-Seidel verfasserin aut Pipob Suwanchaikasem verfasserin aut Shuai Nie verfasserin aut Michael G. Leeming verfasserin aut Michael G. Leeming verfasserin aut Alexandre Augusto Pereira Firmino verfasserin aut Nicholas A. Williamson verfasserin aut Nicholas A. Williamson verfasserin aut Joachim Kopka verfasserin aut Ute Roessner verfasserin aut Berin A. Boughton verfasserin aut Berin A. Boughton verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.656683 kostenfrei https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
allfieldsSound	10.3389/fpls.2021.656683 doi (DE-627)DOAJ068284691 (DE-599)DOAJf87cf1cdfaea410c9ad5d579acff199f DE-627 ger DE-627 rakwb eng SB1-1110 Federico Martinez-Seidel verfasserin aut Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation. Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture Federico Martinez-Seidel verfasserin aut Pipob Suwanchaikasem verfasserin aut Shuai Nie verfasserin aut Michael G. Leeming verfasserin aut Michael G. Leeming verfasserin aut Alexandre Augusto Pereira Firmino verfasserin aut Nicholas A. Williamson verfasserin aut Nicholas A. Williamson verfasserin aut Joachim Kopka verfasserin aut Ute Roessner verfasserin aut Berin A. Boughton verfasserin aut Berin A. Boughton verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2021) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2021 https://doi.org/10.3389/fpls.2021.656683 kostenfrei https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021
language	English
source	In Frontiers in Plant Science 12(2021) volume:12 year:2021
sourceStr	In Frontiers in Plant Science 12(2021) volume:12 year:2021
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Federico Martinez-Seidel @@aut@@ Pipob Suwanchaikasem @@aut@@ Shuai Nie @@aut@@ Michael G. Leeming @@aut@@ Alexandre Augusto Pereira Firmino @@aut@@ Nicholas A. Williamson @@aut@@ Joachim Kopka @@aut@@ Ute Roessner @@aut@@ Berin A. Boughton @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ068284691
language_de	englisch
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callnumber-first	S - Agriculture
author	Federico Martinez-Seidel
spellingShingle	Federico Martinez-Seidel misc SB1-1110 misc Hordeum vulgare misc cv Keel misc abiotic stress misc translation misc ribosomal protein paralog misc fungal priming elicitors misc Plant culture Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems
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topic_title	SB1-1110 Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems Hordeum vulgare cv Keel abiotic stress translation ribosomal protein paralog fungal priming elicitors
topic	misc SB1-1110 misc Hordeum vulgare misc cv Keel misc abiotic stress misc translation misc ribosomal protein paralog misc fungal priming elicitors misc Plant culture
topic_unstemmed	misc SB1-1110 misc Hordeum vulgare misc cv Keel misc abiotic stress misc translation misc ribosomal protein paralog misc fungal priming elicitors misc Plant culture
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title	Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems
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title_full	Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems
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author_browse	Federico Martinez-Seidel Pipob Suwanchaikasem Shuai Nie Michael G. Leeming Alexandre Augusto Pereira Firmino Nicholas A. Williamson Joachim Kopka Ute Roessner Berin A. Boughton
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title_sort	membrane-enriched proteomics link ribosome accumulation and proteome reprogramming with cold acclimation in barley root meristems
callnumber	SB1-1110
title_auth	Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems
abstract	Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation.
abstractGer	Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation.
abstract_unstemmed	Due to their sessile nature, plants rely on root systems to mediate many biotic and abiotic cues. To overcome these challenges, the root proteome is shaped to specific responses. Proteome-wide reprogramming events are magnified in meristems due to their active protein production. Using meristems as a test system, here, we study the major rewiring that plants undergo during cold acclimation. We performed tandem mass tag-based bottom-up quantitative proteomics of two consecutive segments of barley seminal root apexes subjected to suboptimal temperatures. After comparing changes in total and ribosomal protein (RP) fraction-enriched contents with shifts in individual protein abundances, we report ribosome accumulation accompanied by an intricate translational reprogramming in the distal apex zone. Reprogramming ranges from increases in ribosome biogenesis to protein folding factors and suggests roles for cold-specific RP paralogs. Ribosome biogenesis is the largest cellular investment; thus, the vast accumulation of ribosomes and specific translation-related proteins during cold acclimation could imply a divergent ribosomal population that would lead to a proteome shift across the root. Consequently, beyond the translational reprogramming, we report a proteome rewiring. First, triggered protein accumulation includes spliceosome activity in the root tip and a ubiquitous upregulation of glutathione production and S-glutathionylation (S-GSH) assemblage machineries in both root zones. Second, triggered protein depletion includes intrinsically enriched proteins in the tip-adjacent zone, which comprise the plant immune system. In summary, ribosome and translation-related protein accumulation happens concomitantly to a proteome reprogramming in barley root meristems during cold acclimation. The cold-accumulated proteome is functionally implicated in feedbacking transcript to protein translation at both ends and could guide cold acclimation.
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title_short	Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems
url	https://doi.org/10.3389/fpls.2021.656683 https://doaj.org/article/f87cf1cdfaea410c9ad5d579acff199f https://www.frontiersin.org/articles/10.3389/fpls.2021.656683/full https://doaj.org/toc/1664-462X
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author2	Federico Martinez-Seidel Pipob Suwanchaikasem Shuai Nie Michael G. Leeming Alexandre Augusto Pereira Firmino Nicholas A. Williamson Joachim Kopka Ute Roessner Berin A. Boughton
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up_date	2024-07-03T16:54:01.584Z
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Membrane-Enriched Proteomics Link Ribosome Accumulation and Proteome Reprogramming With Cold Acclimation in Barley Root Meristems

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