Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains

Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investi...
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Autor*in:	Seyed Asghar Havaei [verfasserIn] Behnaz Assadbeigi [verfasserIn] Bahram Nasr Esfahani [verfasserIn] Nafiseh Sadat Hoseini [verfasserIn] Nahid Rezaei [verfasserIn] Seyed Rouhollah Havaei [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins

Übergeordnetes Werk:	In: Iranian Journal of Microbiology - Tehran University of Medical Sciences, 2020, 7(2015), 3
Übergeordnetes Werk:	volume:7 ; year:2015 ; number:3

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ068995210

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520			\|a Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type.
650		4	\|a Bovine mastitis
650		4	\|a Staphylococcus aureus
650		4	\|a MRSA
650		4	\|a Staphylococcal enterotoxins
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912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
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912			\|a GBV_ILN_2031
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Indexfelder

author_variant	s a h sah b a ba b n e bne n s h nsh n r nr s r h srh
matchkey_str	article:20084447:2015----::eetoomcadneooignsntpyoocsuessltssoitdihoieattsncaatrztoosahlc
hierarchy_sort_str	2015
callnumber-subject-code	QR
publishDate	2015
allfields	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9 DE-627 ger DE-627 rakwb eng QR1-502 Seyed Asghar Havaei verfasserin aut Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type. Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology Behnaz Assadbeigi verfasserin aut Bahram Nasr Esfahani verfasserin aut Nafiseh Sadat Hoseini verfasserin aut Nahid Rezaei verfasserin aut Seyed Rouhollah Havaei verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 7(2015), 3 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:7 year:2015 number:3 https://doaj.org/article/01443f1c2142441899012c14de0060c9 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/818 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2015 3
spelling	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9 DE-627 ger DE-627 rakwb eng QR1-502 Seyed Asghar Havaei verfasserin aut Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type. Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology Behnaz Assadbeigi verfasserin aut Bahram Nasr Esfahani verfasserin aut Nafiseh Sadat Hoseini verfasserin aut Nahid Rezaei verfasserin aut Seyed Rouhollah Havaei verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 7(2015), 3 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:7 year:2015 number:3 https://doaj.org/article/01443f1c2142441899012c14de0060c9 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/818 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2015 3
allfields_unstemmed	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9 DE-627 ger DE-627 rakwb eng QR1-502 Seyed Asghar Havaei verfasserin aut Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type. Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology Behnaz Assadbeigi verfasserin aut Bahram Nasr Esfahani verfasserin aut Nafiseh Sadat Hoseini verfasserin aut Nahid Rezaei verfasserin aut Seyed Rouhollah Havaei verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 7(2015), 3 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:7 year:2015 number:3 https://doaj.org/article/01443f1c2142441899012c14de0060c9 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/818 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2015 3
allfieldsGer	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9 DE-627 ger DE-627 rakwb eng QR1-502 Seyed Asghar Havaei verfasserin aut Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type. Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology Behnaz Assadbeigi verfasserin aut Bahram Nasr Esfahani verfasserin aut Nafiseh Sadat Hoseini verfasserin aut Nahid Rezaei verfasserin aut Seyed Rouhollah Havaei verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 7(2015), 3 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:7 year:2015 number:3 https://doaj.org/article/01443f1c2142441899012c14de0060c9 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/818 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2015 3
allfieldsSound	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9 DE-627 ger DE-627 rakwb eng QR1-502 Seyed Asghar Havaei verfasserin aut Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type. Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology Behnaz Assadbeigi verfasserin aut Bahram Nasr Esfahani verfasserin aut Nafiseh Sadat Hoseini verfasserin aut Nahid Rezaei verfasserin aut Seyed Rouhollah Havaei verfasserin aut In Iranian Journal of Microbiology Tehran University of Medical Sciences, 2020 7(2015), 3 (DE-627)688077226 (DE-600)2652849-6 20084447 nnns volume:7 year:2015 number:3 https://doaj.org/article/01443f1c2142441899012c14de0060c9 kostenfrei https://ijm.tums.ac.ir/index.php/ijm/article/view/818 kostenfrei https://doaj.org/toc/2008-3289 Journal toc kostenfrei https://doaj.org/toc/2008-4447 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 7 2015 3
language	English
source	In Iranian Journal of Microbiology 7(2015), 3 volume:7 year:2015 number:3
sourceStr	In Iranian Journal of Microbiology 7(2015), 3 volume:7 year:2015 number:3
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins Microbiology
isfreeaccess_bool	true
container_title	Iranian Journal of Microbiology
authorswithroles_txt_mv	Seyed Asghar Havaei @@aut@@ Behnaz Assadbeigi @@aut@@ Bahram Nasr Esfahani @@aut@@ Nafiseh Sadat Hoseini @@aut@@ Nahid Rezaei @@aut@@ Seyed Rouhollah Havaei @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	688077226
id	DOAJ068995210
language_de	englisch
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callnumber-first	Q - Science
author	Seyed Asghar Havaei
spellingShingle	Seyed Asghar Havaei misc QR1-502 misc Bovine mastitis misc Staphylococcus aureus misc MRSA misc Staphylococcal enterotoxins misc Microbiology Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains
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topic_title	QR1-502 Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains Bovine mastitis Staphylococcus aureus MRSA Staphylococcal enterotoxins
topic	misc QR1-502 misc Bovine mastitis misc Staphylococcus aureus misc MRSA misc Staphylococcal enterotoxins misc Microbiology
topic_unstemmed	misc QR1-502 misc Bovine mastitis misc Staphylococcus aureus misc MRSA misc Staphylococcal enterotoxins misc Microbiology
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title	Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains
ctrlnum	(DE-627)DOAJ068995210 (DE-599)DOAJ01443f1c2142441899012c14de0060c9
title_full	Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains
author_sort	Seyed Asghar Havaei
journal	Iranian Journal of Microbiology
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author_browse	Seyed Asghar Havaei Behnaz Assadbeigi Bahram Nasr Esfahani Nafiseh Sadat Hoseini Nahid Rezaei Seyed Rouhollah Havaei
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author-letter	Seyed Asghar Havaei
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title_sort	detection of meca and enterotoxin genes in staphylococcus aureus isolates associated with bovine mastitis and characterization of staphylococcal cassette chromosome mec (sccmec) in mrsa strains
callnumber	QR1-502
title_auth	Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains
abstract	Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type.
abstractGer	Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type.
abstract_unstemmed	Background and Objectives: Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also im- portant. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastitis. Materials and Methods: Four hundred and fifty milk samples were collected. After isolation of Staphylococcus aureus, MRSA strains were detected by cefoxitin disc diffusion and oxacillin agar screening methods. DNA was extracted by phenol – chloroform method and PCR was applied for mecA, sea and seb genes. SCCmec types of mecA gene were identified using multiplex-PCR. Results: Fifty-four (12%) S. aureus were isolated. Out of these, 10 and 9 MRSA strains identified by cefoxitin disc diffusion and oxacillin agar screening methods, respectively. All 10 MRSA isolates identified by cefoxitin disc diffusion, were positive for mecA gene and all of them belonged to SCCmec type IV. The sea genes were detected in 19 isolates and only two isolates were positive for seb genes. One isolate possessed both sea and seb genes. Conclusion: Findings of this study indicated that results of cefoxitin disc diffusion test is in concordance with the PCR for mecA gene and has a higher sensitivity compared to oxacillin agar screening method. Finally, Our findings suggest that enterotoxin A is the dominant type.
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title_short	Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains
url	https://doaj.org/article/01443f1c2142441899012c14de0060c9 https://ijm.tums.ac.ir/index.php/ijm/article/view/818 https://doaj.org/toc/2008-3289 https://doaj.org/toc/2008-4447
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Detection of mecA and enterotoxin genes in Staphylococcus aureus isolates associated with bovine mastitis and characterization of Staphylococcal cassette chromosome mec (SCCmec) in MRSA strains

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