A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W

Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. M...
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Autor*in:	Biao Huang [verfasserIn] Huixin Yu [verfasserIn] Jiandong Bao [verfasserIn] Manda Zhang [verfasserIn] William L Green [verfasserIn] Sing-Yung Wu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Übergeordnetes Werk:	In: Biomarker Insights - SAGE Publishing, 2007, 13(2018)
Übergeordnetes Werk:	volume:13 ; year:2018

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1177/1177271918757484

Katalog-ID:	DOAJ069440573

Internformat


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520			\|a Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.
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700	0		\|a William L Green \|e verfasserin \|4 aut
700	0		\|a Sing-Yung Wu \|e verfasserin \|4 aut
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912			\|a GBV_ILN_2707
912			\|a GBV_ILN_2890
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
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912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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Indexfelder

author_variant	b h bh h y hy j b jb m z mz w l g wlg s y w syw
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allfields	10.1177/1177271918757484 doi (DE-627)DOAJ069440573 (DE-599)DOAJ00747a5a0c3a406688b9444ae1a12991 DE-627 ger DE-627 rakwb eng R5-920 Biao Huang verfasserin aut A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero. Medicine (General) Huixin Yu verfasserin aut Jiandong Bao verfasserin aut Manda Zhang verfasserin aut William L Green verfasserin aut Sing-Yung Wu verfasserin aut In Biomarker Insights SAGE Publishing, 2007 13(2018) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:13 year:2018 https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 kostenfrei https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018
spelling	10.1177/1177271918757484 doi (DE-627)DOAJ069440573 (DE-599)DOAJ00747a5a0c3a406688b9444ae1a12991 DE-627 ger DE-627 rakwb eng R5-920 Biao Huang verfasserin aut A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero. Medicine (General) Huixin Yu verfasserin aut Jiandong Bao verfasserin aut Manda Zhang verfasserin aut William L Green verfasserin aut Sing-Yung Wu verfasserin aut In Biomarker Insights SAGE Publishing, 2007 13(2018) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:13 year:2018 https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 kostenfrei https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018
allfields_unstemmed	10.1177/1177271918757484 doi (DE-627)DOAJ069440573 (DE-599)DOAJ00747a5a0c3a406688b9444ae1a12991 DE-627 ger DE-627 rakwb eng R5-920 Biao Huang verfasserin aut A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero. Medicine (General) Huixin Yu verfasserin aut Jiandong Bao verfasserin aut Manda Zhang verfasserin aut William L Green verfasserin aut Sing-Yung Wu verfasserin aut In Biomarker Insights SAGE Publishing, 2007 13(2018) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:13 year:2018 https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 kostenfrei https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018
allfieldsGer	10.1177/1177271918757484 doi (DE-627)DOAJ069440573 (DE-599)DOAJ00747a5a0c3a406688b9444ae1a12991 DE-627 ger DE-627 rakwb eng R5-920 Biao Huang verfasserin aut A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero. Medicine (General) Huixin Yu verfasserin aut Jiandong Bao verfasserin aut Manda Zhang verfasserin aut William L Green verfasserin aut Sing-Yung Wu verfasserin aut In Biomarker Insights SAGE Publishing, 2007 13(2018) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:13 year:2018 https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 kostenfrei https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018
allfieldsSound	10.1177/1177271918757484 doi (DE-627)DOAJ069440573 (DE-599)DOAJ00747a5a0c3a406688b9444ae1a12991 DE-627 ger DE-627 rakwb eng R5-920 Biao Huang verfasserin aut A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero. Medicine (General) Huixin Yu verfasserin aut Jiandong Bao verfasserin aut Manda Zhang verfasserin aut William L Green verfasserin aut Sing-Yung Wu verfasserin aut In Biomarker Insights SAGE Publishing, 2007 13(2018) (DE-627)519764242 (DE-600)2256754-9 11772719 nnns volume:13 year:2018 https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 kostenfrei https://doi.org/10.1177/1177271918757484 kostenfrei https://doaj.org/toc/1177-2719 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_374 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_2704 GBV_ILN_2707 GBV_ILN_2890 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2018
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source	In Biomarker Insights 13(2018) volume:13 year:2018
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authorswithroles_txt_mv	Biao Huang @@aut@@ Huixin Yu @@aut@@ Jiandong Bao @@aut@@ Manda Zhang @@aut@@ William L Green @@aut@@ Sing-Yung Wu @@aut@@
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Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. 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callnumber-first	R - Medicine
author	Biao Huang
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topic_title	R5-920 A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W
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title	A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W
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title_full	A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W
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author_browse	Biao Huang Huixin Yu Jiandong Bao Manda Zhang William L Green Sing-Yung Wu
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title_sort	homogeneous time-resolved fluorescence immunoassay method for the measurement of compound w
callnumber	R5-920
title_auth	A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W
abstract	Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.
abstractGer	Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.
abstract_unstemmed	Objective: Using compound W (a 3,3′-diiodothyronine sulfate [T 2 S] immuno-crossreactive material)-specific polyclonal antibodies and homogeneous time-resolved fluorescence immunoassay assay techniques (AlphaLISA) to establish an indirect competitive compound W (ICW) quantitative detection method. Method: Photosensitive particles (donor beads) coated with compound W or T 2 S and rabbit anti-W antibody were incubated with biotinylated goat anti-rabbit antibody. This constitutes a detection system with streptavidin-coated acceptor particle. We have optimized the test conditions and evaluated the detection performance. Results: The sensitivity of the method was 5 pg/mL, and the detection range was 5 to 10 000 pg/mL. The intra-assay coefficient of variation averages <10% with stable reproducibility. Conclusions: The ICW-AlphaLISA shows good stability and high sensitivity and can measure a wide range of compound W levels in extracts of maternal serum samples. This may have clinical application to screen congenital hypothyroidism in utero.
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title_short	A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W
url	https://doi.org/10.1177/1177271918757484 https://doaj.org/article/00747a5a0c3a406688b9444ae1a12991 https://doaj.org/toc/1177-2719
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author2	Huixin Yu Jiandong Bao Manda Zhang William L Green Sing-Yung Wu
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up_date	2024-07-03T23:16:00.240Z
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A Homogeneous Time-Resolved Fluorescence Immunoassay Method for the Measurement of Compound W

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