Kloning Gen CRY dari Bacillus turingiensis var. kurstaki

Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thur...
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Autor*in:	Nur Rahmi Ardiani [verfasserIn] Sismindari Sismindari [verfasserIn] Sudjadi Sudjadi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Indonesisch

Erschienen:	1998

Übergeordnetes Werk:	In: Jurnal Perlindungan Tanaman Indonesia - Universitas Gadjah Mada, 2017, 4(1998), 2, Seite 106-111
Übergeordnetes Werk:	volume:4 ; year:1998 ; number:2 ; pages:106-111

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22146/jpti.9918

Katalog-ID:	DOAJ069597863

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520			\|a Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis
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allfields	10.22146/jpti.9918 doi (DE-627)DOAJ069597863 (DE-599)DOAJ2abee7d75dae45209cbffeba62b8aca8 DE-627 ger DE-627 rakwb eng ind S1-972 SB1-1110 Nur Rahmi Ardiani verfasserin aut Kloning Gen CRY dari Bacillus turingiensis var. kurstaki 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis Agriculture (General) Plant culture Sismindari Sismindari verfasserin aut Sudjadi Sudjadi verfasserin aut In Jurnal Perlindungan Tanaman Indonesia Universitas Gadjah Mada, 2017 4(1998), 2, Seite 106-111 (DE-627)1667436341 25484788 nnns volume:4 year:1998 number:2 pages:106-111 https://doi.org/10.22146/jpti.9918 kostenfrei https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 kostenfrei https://jurnal.ugm.ac.id/jpti/article/view/9918 kostenfrei https://doaj.org/toc/1410-1637 Journal toc kostenfrei https://doaj.org/toc/2548-4788 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 1998 2 106-111
spelling	10.22146/jpti.9918 doi (DE-627)DOAJ069597863 (DE-599)DOAJ2abee7d75dae45209cbffeba62b8aca8 DE-627 ger DE-627 rakwb eng ind S1-972 SB1-1110 Nur Rahmi Ardiani verfasserin aut Kloning Gen CRY dari Bacillus turingiensis var. kurstaki 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis Agriculture (General) Plant culture Sismindari Sismindari verfasserin aut Sudjadi Sudjadi verfasserin aut In Jurnal Perlindungan Tanaman Indonesia Universitas Gadjah Mada, 2017 4(1998), 2, Seite 106-111 (DE-627)1667436341 25484788 nnns volume:4 year:1998 number:2 pages:106-111 https://doi.org/10.22146/jpti.9918 kostenfrei https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 kostenfrei https://jurnal.ugm.ac.id/jpti/article/view/9918 kostenfrei https://doaj.org/toc/1410-1637 Journal toc kostenfrei https://doaj.org/toc/2548-4788 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 1998 2 106-111
allfields_unstemmed	10.22146/jpti.9918 doi (DE-627)DOAJ069597863 (DE-599)DOAJ2abee7d75dae45209cbffeba62b8aca8 DE-627 ger DE-627 rakwb eng ind S1-972 SB1-1110 Nur Rahmi Ardiani verfasserin aut Kloning Gen CRY dari Bacillus turingiensis var. kurstaki 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis Agriculture (General) Plant culture Sismindari Sismindari verfasserin aut Sudjadi Sudjadi verfasserin aut In Jurnal Perlindungan Tanaman Indonesia Universitas Gadjah Mada, 2017 4(1998), 2, Seite 106-111 (DE-627)1667436341 25484788 nnns volume:4 year:1998 number:2 pages:106-111 https://doi.org/10.22146/jpti.9918 kostenfrei https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 kostenfrei https://jurnal.ugm.ac.id/jpti/article/view/9918 kostenfrei https://doaj.org/toc/1410-1637 Journal toc kostenfrei https://doaj.org/toc/2548-4788 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 1998 2 106-111
allfieldsGer	10.22146/jpti.9918 doi (DE-627)DOAJ069597863 (DE-599)DOAJ2abee7d75dae45209cbffeba62b8aca8 DE-627 ger DE-627 rakwb eng ind S1-972 SB1-1110 Nur Rahmi Ardiani verfasserin aut Kloning Gen CRY dari Bacillus turingiensis var. kurstaki 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis Agriculture (General) Plant culture Sismindari Sismindari verfasserin aut Sudjadi Sudjadi verfasserin aut In Jurnal Perlindungan Tanaman Indonesia Universitas Gadjah Mada, 2017 4(1998), 2, Seite 106-111 (DE-627)1667436341 25484788 nnns volume:4 year:1998 number:2 pages:106-111 https://doi.org/10.22146/jpti.9918 kostenfrei https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 kostenfrei https://jurnal.ugm.ac.id/jpti/article/view/9918 kostenfrei https://doaj.org/toc/1410-1637 Journal toc kostenfrei https://doaj.org/toc/2548-4788 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 1998 2 106-111
allfieldsSound	10.22146/jpti.9918 doi (DE-627)DOAJ069597863 (DE-599)DOAJ2abee7d75dae45209cbffeba62b8aca8 DE-627 ger DE-627 rakwb eng ind S1-972 SB1-1110 Nur Rahmi Ardiani verfasserin aut Kloning Gen CRY dari Bacillus turingiensis var. kurstaki 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis Agriculture (General) Plant culture Sismindari Sismindari verfasserin aut Sudjadi Sudjadi verfasserin aut In Jurnal Perlindungan Tanaman Indonesia Universitas Gadjah Mada, 2017 4(1998), 2, Seite 106-111 (DE-627)1667436341 25484788 nnns volume:4 year:1998 number:2 pages:106-111 https://doi.org/10.22146/jpti.9918 kostenfrei https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 kostenfrei https://jurnal.ugm.ac.id/jpti/article/view/9918 kostenfrei https://doaj.org/toc/1410-1637 Journal toc kostenfrei https://doaj.org/toc/2548-4788 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 1998 2 106-111
language	English Indonesian
source	In Jurnal Perlindungan Tanaman Indonesia 4(1998), 2, Seite 106-111 volume:4 year:1998 number:2 pages:106-111
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callnumber-first	S - Agriculture
author	Nur Rahmi Ardiani
spellingShingle	Nur Rahmi Ardiani misc S1-972 misc SB1-1110 misc Agriculture (General) misc Plant culture Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
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topic_title	S1-972 SB1-1110 Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
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title	Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
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title_full	Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
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title_sort	kloning gen cry dari bacillus turingiensis var. kurstaki
callnumber	S1-972
title_auth	Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
abstract	Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis
abstractGer	Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis
abstract_unstemmed	Bacillus thuringiensis is an entomopathogenic organism. The pathogenic effect is caused by crystalline protein, δ-endotoxin, which is encoded by the cry gene. The aim of this study was to clone the cry gene from B. thuringiensis var. kurstaki with a genomic library approach. HindIII digested B. thuringiensis total DNA was ligated to HindIII digested of pUC19, and then used to transform Escherichia coli DH5α. Selection of transformans carrying recombinant was done by α-complementation and a recombinant clone containing the cry gene was further screened by non-radioactive hybridization method using a probe synthesized from the conserved region of the published cry genes. The result suggested that two recombinant clones with the insert size of 3.4 kb and 2.0 kb, respectively, carrying the cry gene. Key words: cloning, cry gene, Bacillus thuringiensis
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title_short	Kloning Gen CRY dari Bacillus turingiensis var. kurstaki
url	https://doi.org/10.22146/jpti.9918 https://doaj.org/article/2abee7d75dae45209cbffeba62b8aca8 https://jurnal.ugm.ac.id/jpti/article/view/9918 https://doaj.org/toc/1410-1637 https://doaj.org/toc/2548-4788
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