Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor

Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive,...
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Gespeichert in:

Autor*in:	Francesco Arcadio [verfasserIn] Luigi Zeni [verfasserIn] Chiara Perri [verfasserIn] Girolamo D’Agostino [verfasserIn] Giudo Chiaretti [verfasserIn] Giovanni Porto [verfasserIn] Aldo Minardo [verfasserIn] Nunzio Cennamo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) optical sensors bovine serum albumin (BSA) protein

Übergeordnetes Werk:	In: Chemosensors - MDPI AG, 2013, 9(2021), 8, p 218
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:8, p 218

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/chemosensors9080218

Katalog-ID:	DOAJ070157294

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authorswithroles_txt_mv	Francesco Arcadio @@aut@@ Luigi Zeni @@aut@@ Chiara Perri @@aut@@ Girolamo D’Agostino @@aut@@ Giudo Chiaretti @@aut@@ Giovanni Porto @@aut@@ Aldo Minardo @@aut@@ Nunzio Cennamo @@aut@@
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callnumber-first	Q - Science
author	Francesco Arcadio
spellingShingle	Francesco Arcadio misc QD415-436 misc surface plasmon resonance (SPR) misc molecularly imprinted polymers (MIPs) misc optical sensors misc bovine serum albumin (BSA) protein misc Biochemistry Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
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topic_title	QD415-436 Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor surface plasmon resonance (SPR) molecularly imprinted polymers (MIPs) optical sensors bovine serum albumin (BSA) protein
topic	misc QD415-436 misc surface plasmon resonance (SPR) misc molecularly imprinted polymers (MIPs) misc optical sensors misc bovine serum albumin (BSA) protein misc Biochemistry
topic_unstemmed	misc QD415-436 misc surface plasmon resonance (SPR) misc molecularly imprinted polymers (MIPs) misc optical sensors misc bovine serum albumin (BSA) protein misc Biochemistry
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title	Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
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title_full	Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
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author_browse	Francesco Arcadio Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo
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title_sort	bovine serum albumin protein detection by a removable spr chip combined with a specific mip receptor
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title_auth	Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
abstract	Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10<sup<−9</sup< M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.
abstractGer	Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10<sup<−9</sup< M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.
abstract_unstemmed	Nowadays, the development of simple, fast, and low-cost selective sensors to detect substances of interest is of great importance in several application fields. Among this kind of sensors, those based on surface plasmon resonance (SPR) represent a promising category, since they are highly sensitive, versatile, and label-free. In this work, an SPR probe, based on a poly(methyl methacrylate) (PMMA) slab waveguide covered by a gold nanofilm, combined with a specific molecularly imprinted polymer (MIP) receptor for bovine serum albumin (BSA) protein, has been realized and experimentally characterized. The obtained experimental results have shown a limit of detection (LOD) equal to about 8.5 × 10<sup<−9</sup< M. This value is smaller than the one achieved by another SPR probe, based on a D-shaped plastic optical fiber (POF), functionalized with the same MIP receptor; more specifically, the obtained LOD was reduced by about three orders of magnitude with respect to the POF configuration. Moreover, concerning the D-shaped POF configuration, no manufacturing process is present in the proposed sensor configuration. In addition, fibers are used only to connect the simple sensor chip with a light source and a detector, promoting a bio-chemical sensing approach based on disposable, low-cost, and removable chips.
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title_short	Bovine Serum Albumin Protein Detection by a Removable SPR Chip Combined with a Specific MIP Receptor
url	https://doi.org/10.3390/chemosensors9080218 https://doaj.org/article/787c8a85761443698971bb1005ed7407 https://www.mdpi.com/2227-9040/9/8/218 https://doaj.org/toc/2227-9040
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author2	Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo
author2Str	Luigi Zeni Chiara Perri Girolamo D’Agostino Giudo Chiaretti Giovanni Porto Aldo Minardo Nunzio Cennamo
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