Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows

Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) m...
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Autor*in:	Denis Revskij [verfasserIn] Susanne Haubold [verfasserIn] Torsten Viergutz [verfasserIn] Claudia Kröger-Koch [verfasserIn] Armin Tuchscherer [verfasserIn] Hermine Kienberger [verfasserIn] Michael Rychlik [verfasserIn] Arnulf Tröscher [verfasserIn] Harald M. Hammon [verfasserIn] Hans-Joachim Schuberth [verfasserIn] Manfred Mielenz [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 20(2019), 11, p 2769
Übergeordnetes Werk:	volume:20 ; year:2019 ; number:11, p 2769

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms20112769

Katalog-ID:	DOAJ070448949

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520			\|a Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.
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allfields	10.3390/ijms20112769 doi (DE-627)DOAJ070448949 (DE-599)DOAJ1d7bf1cb9439419cba959f665849d7e6 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Denis Revskij verfasserin aut Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC. dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry Susanne Haubold verfasserin aut Torsten Viergutz verfasserin aut Claudia Kröger-Koch verfasserin aut Armin Tuchscherer verfasserin aut Hermine Kienberger verfasserin aut Michael Rychlik verfasserin aut Arnulf Tröscher verfasserin aut Harald M. Hammon verfasserin aut Hans-Joachim Schuberth verfasserin aut Manfred Mielenz verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 11, p 2769 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:11, p 2769 https://doi.org/10.3390/ijms20112769 kostenfrei https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 kostenfrei https://www.mdpi.com/1422-0067/20/11/2769 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 11, p 2769
spelling	10.3390/ijms20112769 doi (DE-627)DOAJ070448949 (DE-599)DOAJ1d7bf1cb9439419cba959f665849d7e6 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Denis Revskij verfasserin aut Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC. dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry Susanne Haubold verfasserin aut Torsten Viergutz verfasserin aut Claudia Kröger-Koch verfasserin aut Armin Tuchscherer verfasserin aut Hermine Kienberger verfasserin aut Michael Rychlik verfasserin aut Arnulf Tröscher verfasserin aut Harald M. Hammon verfasserin aut Hans-Joachim Schuberth verfasserin aut Manfred Mielenz verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 11, p 2769 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:11, p 2769 https://doi.org/10.3390/ijms20112769 kostenfrei https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 kostenfrei https://www.mdpi.com/1422-0067/20/11/2769 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 11, p 2769
allfields_unstemmed	10.3390/ijms20112769 doi (DE-627)DOAJ070448949 (DE-599)DOAJ1d7bf1cb9439419cba959f665849d7e6 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Denis Revskij verfasserin aut Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC. dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry Susanne Haubold verfasserin aut Torsten Viergutz verfasserin aut Claudia Kröger-Koch verfasserin aut Armin Tuchscherer verfasserin aut Hermine Kienberger verfasserin aut Michael Rychlik verfasserin aut Arnulf Tröscher verfasserin aut Harald M. Hammon verfasserin aut Hans-Joachim Schuberth verfasserin aut Manfred Mielenz verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 11, p 2769 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:11, p 2769 https://doi.org/10.3390/ijms20112769 kostenfrei https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 kostenfrei https://www.mdpi.com/1422-0067/20/11/2769 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 11, p 2769
allfieldsGer	10.3390/ijms20112769 doi (DE-627)DOAJ070448949 (DE-599)DOAJ1d7bf1cb9439419cba959f665849d7e6 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Denis Revskij verfasserin aut Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC. dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry Susanne Haubold verfasserin aut Torsten Viergutz verfasserin aut Claudia Kröger-Koch verfasserin aut Armin Tuchscherer verfasserin aut Hermine Kienberger verfasserin aut Michael Rychlik verfasserin aut Arnulf Tröscher verfasserin aut Harald M. Hammon verfasserin aut Hans-Joachim Schuberth verfasserin aut Manfred Mielenz verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 11, p 2769 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:11, p 2769 https://doi.org/10.3390/ijms20112769 kostenfrei https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 kostenfrei https://www.mdpi.com/1422-0067/20/11/2769 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 11, p 2769
allfieldsSound	10.3390/ijms20112769 doi (DE-627)DOAJ070448949 (DE-599)DOAJ1d7bf1cb9439419cba959f665849d7e6 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Denis Revskij verfasserin aut Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC. dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry Susanne Haubold verfasserin aut Torsten Viergutz verfasserin aut Claudia Kröger-Koch verfasserin aut Armin Tuchscherer verfasserin aut Hermine Kienberger verfasserin aut Michael Rychlik verfasserin aut Arnulf Tröscher verfasserin aut Harald M. Hammon verfasserin aut Hans-Joachim Schuberth verfasserin aut Manfred Mielenz verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 11, p 2769 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:11, p 2769 https://doi.org/10.3390/ijms20112769 kostenfrei https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 kostenfrei https://www.mdpi.com/1422-0067/20/11/2769 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 11, p 2769
language	English
source	In International Journal of Molecular Sciences 20(2019), 11, p 2769 volume:20 year:2019 number:11, p 2769
sourceStr	In International Journal of Molecular Sciences 20(2019), 11, p 2769 volume:20 year:2019 number:11, p 2769
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Denis Revskij @@aut@@ Susanne Haubold @@aut@@ Torsten Viergutz @@aut@@ Claudia Kröger-Koch @@aut@@ Armin Tuchscherer @@aut@@ Hermine Kienberger @@aut@@ Michael Rychlik @@aut@@ Arnulf Tröscher @@aut@@ Harald M. Hammon @@aut@@ Hans-Joachim Schuberth @@aut@@ Manfred Mielenz @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ070448949
language_de	englisch
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callnumber-first	Q - Science
author	Denis Revskij
spellingShingle	Denis Revskij misc QH301-705.5 misc QD1-999 misc dairy cow misc red blood cell misc dietary fatty acids misc n-3 fatty acids misc n-6 fatty acids misc flotillin-1 misc pannexin-1 misc adenosine triphosphate release misc Biology (General) misc Chemistry Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
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topic_title	QH301-705.5 QD1-999 Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows dairy cow red blood cell dietary fatty acids n-3 fatty acids n-6 fatty acids flotillin-1 pannexin-1 adenosine triphosphate release
topic	misc QH301-705.5 misc QD1-999 misc dairy cow misc red blood cell misc dietary fatty acids misc n-3 fatty acids misc n-6 fatty acids misc flotillin-1 misc pannexin-1 misc adenosine triphosphate release misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc dairy cow misc red blood cell misc dietary fatty acids misc n-3 fatty acids misc n-6 fatty acids misc flotillin-1 misc pannexin-1 misc adenosine triphosphate release misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc dairy cow misc red blood cell misc dietary fatty acids misc n-3 fatty acids misc n-6 fatty acids misc flotillin-1 misc pannexin-1 misc adenosine triphosphate release misc Biology (General) misc Chemistry
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title	Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
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title_full	Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
author_sort	Denis Revskij
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author_browse	Denis Revskij Susanne Haubold Torsten Viergutz Claudia Kröger-Koch Armin Tuchscherer Hermine Kienberger Michael Rychlik Arnulf Tröscher Harald M. Hammon Hans-Joachim Schuberth Manfred Mielenz
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title_sort	dietary fatty acids affect red blood cell membrane composition and red blood cell atp release in dairy cows
callnumber	QH301-705.5
title_auth	Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
abstract	Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.
abstractGer	Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.
abstract_unstemmed	Diets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed−safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.
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title_short	Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows
url	https://doi.org/10.3390/ijms20112769 https://doaj.org/article/1d7bf1cb9439419cba959f665849d7e6 https://www.mdpi.com/1422-0067/20/11/2769 https://doaj.org/toc/1422-0067
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Dietary Fatty Acids Affect Red Blood Cell Membrane Composition and Red Blood Cell ATP Release in Dairy Cows

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