Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study

Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolar...
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Autor*in:	Amulya Vanapatla [verfasserIn] Harikumar Vemisetty [verfasserIn] Rajani Punna [verfasserIn] Chandrasekhar Veeramachineni [verfasserIn] Ravichandra Polavarapu Venkata [verfasserIn] Jaya Nagendra Krishna Muppala [verfasserIn] Ramkiran Dandolu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer

Übergeordnetes Werk:	In: Journal of Clinical and Diagnostic Research - JCDR Research and Publications Private Limited, 2009, 10(2016), 4, Seite ZC69-ZC72
Übergeordnetes Werk:	volume:10 ; year:2016 ; number:4 ; pages:ZC69-ZC72

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.7860/JCDR/2016/17228.7645

Katalog-ID:	DOAJ070713456

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520			\|a Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
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allfields	10.7860/JCDR/2016/17228.7645 doi (DE-627)DOAJ070713456 (DE-599)DOAJc26419c1124246629f41651de1bdec86 DE-627 ger DE-627 rakwb eng Amulya Vanapatla verfasserin aut Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly. apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer Medicine R Harikumar Vemisetty verfasserin aut Rajani Punna verfasserin aut Chandrasekhar Veeramachineni verfasserin aut Ravichandra Polavarapu Venkata verfasserin aut Jaya Nagendra Krishna Muppala verfasserin aut Ramkiran Dandolu verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 10(2016), 4, Seite ZC69-ZC72 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:10 year:2016 number:4 pages:ZC69-ZC72 https://doi.org/10.7860/JCDR/2016/17228.7645 kostenfrei https://doaj.org/article/c26419c1124246629f41651de1bdec86 kostenfrei https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2016 4 ZC69-ZC72
spelling	10.7860/JCDR/2016/17228.7645 doi (DE-627)DOAJ070713456 (DE-599)DOAJc26419c1124246629f41651de1bdec86 DE-627 ger DE-627 rakwb eng Amulya Vanapatla verfasserin aut Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly. apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer Medicine R Harikumar Vemisetty verfasserin aut Rajani Punna verfasserin aut Chandrasekhar Veeramachineni verfasserin aut Ravichandra Polavarapu Venkata verfasserin aut Jaya Nagendra Krishna Muppala verfasserin aut Ramkiran Dandolu verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 10(2016), 4, Seite ZC69-ZC72 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:10 year:2016 number:4 pages:ZC69-ZC72 https://doi.org/10.7860/JCDR/2016/17228.7645 kostenfrei https://doaj.org/article/c26419c1124246629f41651de1bdec86 kostenfrei https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2016 4 ZC69-ZC72
allfields_unstemmed	10.7860/JCDR/2016/17228.7645 doi (DE-627)DOAJ070713456 (DE-599)DOAJc26419c1124246629f41651de1bdec86 DE-627 ger DE-627 rakwb eng Amulya Vanapatla verfasserin aut Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly. apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer Medicine R Harikumar Vemisetty verfasserin aut Rajani Punna verfasserin aut Chandrasekhar Veeramachineni verfasserin aut Ravichandra Polavarapu Venkata verfasserin aut Jaya Nagendra Krishna Muppala verfasserin aut Ramkiran Dandolu verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 10(2016), 4, Seite ZC69-ZC72 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:10 year:2016 number:4 pages:ZC69-ZC72 https://doi.org/10.7860/JCDR/2016/17228.7645 kostenfrei https://doaj.org/article/c26419c1124246629f41651de1bdec86 kostenfrei https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2016 4 ZC69-ZC72
allfieldsGer	10.7860/JCDR/2016/17228.7645 doi (DE-627)DOAJ070713456 (DE-599)DOAJc26419c1124246629f41651de1bdec86 DE-627 ger DE-627 rakwb eng Amulya Vanapatla verfasserin aut Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly. apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer Medicine R Harikumar Vemisetty verfasserin aut Rajani Punna verfasserin aut Chandrasekhar Veeramachineni verfasserin aut Ravichandra Polavarapu Venkata verfasserin aut Jaya Nagendra Krishna Muppala verfasserin aut Ramkiran Dandolu verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 10(2016), 4, Seite ZC69-ZC72 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:10 year:2016 number:4 pages:ZC69-ZC72 https://doi.org/10.7860/JCDR/2016/17228.7645 kostenfrei https://doaj.org/article/c26419c1124246629f41651de1bdec86 kostenfrei https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2016 4 ZC69-ZC72
allfieldsSound	10.7860/JCDR/2016/17228.7645 doi (DE-627)DOAJ070713456 (DE-599)DOAJc26419c1124246629f41651de1bdec86 DE-627 ger DE-627 rakwb eng Amulya Vanapatla verfasserin aut Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly. apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer Medicine R Harikumar Vemisetty verfasserin aut Rajani Punna verfasserin aut Chandrasekhar Veeramachineni verfasserin aut Ravichandra Polavarapu Venkata verfasserin aut Jaya Nagendra Krishna Muppala verfasserin aut Ramkiran Dandolu verfasserin aut In Journal of Clinical and Diagnostic Research JCDR Research and Publications Private Limited, 2009 10(2016), 4, Seite ZC69-ZC72 (DE-627)789478048 (DE-600)2775283-5 0973709X nnns volume:10 year:2016 number:4 pages:ZC69-ZC72 https://doi.org/10.7860/JCDR/2016/17228.7645 kostenfrei https://doaj.org/article/c26419c1124246629f41651de1bdec86 kostenfrei https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf kostenfrei https://doaj.org/toc/2249-782X Journal toc kostenfrei https://doaj.org/toc/0973-709X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2016 4 ZC69-ZC72
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author	Amulya Vanapatla
spellingShingle	Amulya Vanapatla misc apexit plus sealer misc ah plus sealer misc triple antibiotic mixture misc zinc oxide eugenol sealer misc Medicine misc R Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
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topic_title	Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study apexit plus sealer ah plus sealer triple antibiotic mixture zinc oxide eugenol sealer
topic	misc apexit plus sealer misc ah plus sealer misc triple antibiotic mixture misc zinc oxide eugenol sealer misc Medicine misc R
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title	Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
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title_full	Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
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title_sort	comparative evaluation of antimicrobial effect of three endodontic sealers with and without antibiotics – an in-vitro study
title_auth	Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
abstract	Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
abstractGer	Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
abstract_unstemmed	Introduction: Root canal sealers with good sealing ability and antimicrobial activity are desired to entomb the surviving microorganisms. Aim: Aim of the present study is to evaluate the antimicrobial effect of different sealers mixed with antibiotics. Materials and Methods: Sixty extracted premolars were taken and were cut coronally & apically such that 7mm of tooth specimen was prepared. They were sterilized by autoclaving, inoculated with E.faecalis and incubated for three weeks. The specimens were divided into six groups of 10 each. Group 1 – ZnOE, Group 2 – ZnOE +TAM, Group 3 – Apexit Plus, Group 4 – Apexit Plus +TAM, Group 5 – AH Plus, Group 6 – AH Plus + TAM. Bacterial growth in the each specimen was calculated before & after sealer application and noted as initial & final colony count. Antimicrobial effect of each sealer was measured by calculating the Percentage Reduction in Colony Count (%RCC). One way analysis of variance and post hoc tests were used for statistical analysis. Results: ZnOE + TAM group showed maximum antibacterial effect among the sealers tested and AH Plus sealer showed least antimicrobial effect. Conclusion: When the sealers were mixed with triple antibiotic mixture the antimicrobial effect was increased significantly.
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title_short	Comparative Evaluation of Antimicrobial Effect of Three Endodontic Sealers with and Without Antibiotics – An In-vitro Study
url	https://doi.org/10.7860/JCDR/2016/17228.7645 https://doaj.org/article/c26419c1124246629f41651de1bdec86 https://jcdr.net/articles/PDF/7645/17228_CE(EK)_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf https://doaj.org/toc/2249-782X https://doaj.org/toc/0973-709X
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