Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.
Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying...
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| Autor*in: |
Sherif Fathy Eid El-Sayed El-Gioushy [verfasserIn] Abdul Kareem [verfasserIn] Mohamed Hemdan Mohamed Baiea [verfasserIn] |
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| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch ; Spanisch ; Portugiesisch |
| Schlagwörter: |
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In: Revista Brasileira de Fruticultura - Sociedade Brasileira de Fruticultura, 2004 |
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| Links: |
|---|
| DOI / URN: |
10.1590/0100-29452019561 |
|---|
| Katalog-ID: |
DOAJ070974748 |
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| 520 | |a Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. | ||
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10.1590/0100-29452019561 doi (DE-627)DOAJ070974748 (DE-599)DOAJ9e22cbe98897426098ef611b6ae28254 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Sherif Fathy Eid El-Sayed El-Gioushy verfasserin aut Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. Maçã Hibridização Celulase Pectianase Macrozyme Plant culture Abdul Kareem verfasserin aut Mohamed Hemdan Mohamed Baiea verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns https://doi.org/10.1590/0100-29452019561 kostenfrei https://doaj.org/article/9e22cbe98897426098ef611b6ae28254 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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10.1590/0100-29452019561 doi (DE-627)DOAJ070974748 (DE-599)DOAJ9e22cbe98897426098ef611b6ae28254 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Sherif Fathy Eid El-Sayed El-Gioushy verfasserin aut Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. Maçã Hibridização Celulase Pectianase Macrozyme Plant culture Abdul Kareem verfasserin aut Mohamed Hemdan Mohamed Baiea verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns https://doi.org/10.1590/0100-29452019561 kostenfrei https://doaj.org/article/9e22cbe98897426098ef611b6ae28254 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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10.1590/0100-29452019561 doi (DE-627)DOAJ070974748 (DE-599)DOAJ9e22cbe98897426098ef611b6ae28254 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Sherif Fathy Eid El-Sayed El-Gioushy verfasserin aut Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. Maçã Hibridização Celulase Pectianase Macrozyme Plant culture Abdul Kareem verfasserin aut Mohamed Hemdan Mohamed Baiea verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns https://doi.org/10.1590/0100-29452019561 kostenfrei https://doaj.org/article/9e22cbe98897426098ef611b6ae28254 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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10.1590/0100-29452019561 doi (DE-627)DOAJ070974748 (DE-599)DOAJ9e22cbe98897426098ef611b6ae28254 DE-627 ger DE-627 rakwb eng spa por SB1-1110 Sherif Fathy Eid El-Sayed El-Gioushy verfasserin aut Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. Maçã Hibridização Celulase Pectianase Macrozyme Plant culture Abdul Kareem verfasserin aut Mohamed Hemdan Mohamed Baiea verfasserin aut In Revista Brasileira de Fruticultura Sociedade Brasileira de Fruticultura, 2004 (DE-627)363749667 (DE-600)2105182-3 18069967 nnns https://doi.org/10.1590/0100-29452019561 kostenfrei https://doaj.org/article/9e22cbe98897426098ef611b6ae28254 kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803&lng=en&tlng=en kostenfrei https://doaj.org/toc/1806-9967 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR |
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Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. |
| abstract |
Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. |
| abstractGer |
Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. |
| abstract_unstemmed |
Abstract To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA |
| title_short |
Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv. |
| url |
https://doi.org/10.1590/0100-29452019561 https://doaj.org/article/9e22cbe98897426098ef611b6ae28254 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400803&lng=en&tlng=en https://doaj.org/toc/1806-9967 |
| remote_bool |
true |
| author2 |
Abdul Kareem Mohamed Hemdan Mohamed Baiea |
| author2Str |
Abdul Kareem Mohamed Hemdan Mohamed Baiea |
| ppnlink |
363749667 |
| callnumber-subject |
SB - Plant Culture |
| mediatype_str_mv |
c |
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true |
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false |
| doi_str |
10.1590/0100-29452019561 |
| callnumber-a |
SB1-1110 |
| up_date |
2024-07-03T17:45:16.481Z |
| _version_ |
1803580831413829632 |
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7.401681 |