A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straight...
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Autor*in:	Jacob Lam [verfasserIn] Prasanna Katti [verfasserIn] Michelle Biete [verfasserIn] Margaret Mungai [verfasserIn] Salma AshShareef [verfasserIn] Kit Neikirk [verfasserIn] Edgar Garza Lopez [verfasserIn] Zer Vue [verfasserIn] Trace A. Christensen [verfasserIn] Heather K. Beasley [verfasserIn] Taylor A. Rodman [verfasserIn] Sandra A. Murray [verfasserIn] Jeffrey L. Salisbury [verfasserIn] Brian Glancy [verfasserIn] Jianqiang Shao [verfasserIn] Renata O. Pereira [verfasserIn] E. Dale Abel [verfasserIn] Antentor Hinton [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	cristae image analysis Mitochondria Endoplasmic Reticulum Contacts (MERCs) image processing ImageJ TEM analysis

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 10(2021), 9, p 2177
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:9, p 2177

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells10092177

Katalog-ID:	DOAJ071436839

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spelling	10.3390/cells10092177 doi (DE-627)DOAJ071436839 (DE-599)DOAJ2bd6a86286e24c87a545789cdc785866 DE-627 ger DE-627 rakwb eng QH573-671 Jacob Lam verfasserin aut A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software <i<ImageJ</i<, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER. cristae image analysis Mitochondria Endoplasmic Reticulum Contacts (MERCs) image processing ImageJ TEM analysis Cytology Prasanna Katti verfasserin aut Michelle Biete verfasserin aut Margaret Mungai verfasserin aut Salma AshShareef verfasserin aut Kit Neikirk verfasserin aut Edgar Garza Lopez verfasserin aut Zer Vue verfasserin aut Trace A. Christensen verfasserin aut Heather K. Beasley verfasserin aut Taylor A. Rodman verfasserin aut Sandra A. Murray verfasserin aut Jeffrey L. Salisbury verfasserin aut Brian Glancy verfasserin aut Jianqiang Shao verfasserin aut Renata O. Pereira verfasserin aut E. Dale Abel verfasserin aut Antentor Hinton verfasserin aut In Cells MDPI AG, 2012 10(2021), 9, p 2177 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:9, p 2177 https://doi.org/10.3390/cells10092177 kostenfrei https://doaj.org/article/2bd6a86286e24c87a545789cdc785866 kostenfrei https://www.mdpi.com/2073-4409/10/9/2177 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 9, p 2177
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authorswithroles_txt_mv	Jacob Lam @@aut@@ Prasanna Katti @@aut@@ Michelle Biete @@aut@@ Margaret Mungai @@aut@@ Salma AshShareef @@aut@@ Kit Neikirk @@aut@@ Edgar Garza Lopez @@aut@@ Zer Vue @@aut@@ Trace A. Christensen @@aut@@ Heather K. Beasley @@aut@@ Taylor A. Rodman @@aut@@ Sandra A. Murray @@aut@@ Jeffrey L. Salisbury @@aut@@ Brian Glancy @@aut@@ Jianqiang Shao @@aut@@ Renata O. Pereira @@aut@@ E. Dale Abel @@aut@@ Antentor Hinton @@aut@@
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author	Jacob Lam
spellingShingle	Jacob Lam misc QH573-671 misc cristae misc image analysis misc Mitochondria Endoplasmic Reticulum Contacts (MERCs) misc image processing misc ImageJ misc TEM analysis misc Cytology A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
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topic_title	QH573-671 A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ cristae image analysis Mitochondria Endoplasmic Reticulum Contacts (MERCs) image processing ImageJ TEM analysis
topic	misc QH573-671 misc cristae misc image analysis misc Mitochondria Endoplasmic Reticulum Contacts (MERCs) misc image processing misc ImageJ misc TEM analysis misc Cytology
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title	A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
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title_full	A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
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title_auth	A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
abstract	Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software <i<ImageJ</i<, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.
abstractGer	Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software <i<ImageJ</i<, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.
abstract_unstemmed	Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software <i<ImageJ</i<, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.
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title_short	A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
url	https://doi.org/10.3390/cells10092177 https://doaj.org/article/2bd6a86286e24c87a545789cdc785866 https://www.mdpi.com/2073-4409/10/9/2177 https://doaj.org/toc/2073-4409
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up_date	2024-07-03T20:15:55.331Z
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A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ

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