Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype

In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body...
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Autor*in:	Fahd Alhamdan [verfasserIn] Leigh M. Marsh [verfasserIn] Frauke Pedersen [verfasserIn] Bilal Alashkar Alhamwe [verfasserIn] Clemens Thölken [verfasserIn] Petra Ina Pfefferle [verfasserIn] Thomas Bahmer [verfasserIn] Timm Greulich [verfasserIn] Daniel P. Potaczek [verfasserIn] Holger Garn [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	low type-2 asthma obesity asthma phenotypes CD4 transcriptomics

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 18, p 10144
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:18, p 10144

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms221810144

Katalog-ID:	DOAJ071498192

Internformat


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allfields	10.3390/ijms221810144 doi (DE-627)DOAJ071498192 (DE-599)DOAJe5b92328a8b04109b27b8bacc43aaccb DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Fahd Alhamdan verfasserin aut Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches. low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry Leigh M. Marsh verfasserin aut Frauke Pedersen verfasserin aut Bilal Alashkar Alhamwe verfasserin aut Clemens Thölken verfasserin aut Petra Ina Pfefferle verfasserin aut Thomas Bahmer verfasserin aut Timm Greulich verfasserin aut Daniel P. Potaczek verfasserin aut Holger Garn verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 18, p 10144 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:18, p 10144 https://doi.org/10.3390/ijms221810144 kostenfrei https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb kostenfrei https://www.mdpi.com/1422-0067/22/18/10144 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 18, p 10144
spelling	10.3390/ijms221810144 doi (DE-627)DOAJ071498192 (DE-599)DOAJe5b92328a8b04109b27b8bacc43aaccb DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Fahd Alhamdan verfasserin aut Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches. low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry Leigh M. Marsh verfasserin aut Frauke Pedersen verfasserin aut Bilal Alashkar Alhamwe verfasserin aut Clemens Thölken verfasserin aut Petra Ina Pfefferle verfasserin aut Thomas Bahmer verfasserin aut Timm Greulich verfasserin aut Daniel P. Potaczek verfasserin aut Holger Garn verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 18, p 10144 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:18, p 10144 https://doi.org/10.3390/ijms221810144 kostenfrei https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb kostenfrei https://www.mdpi.com/1422-0067/22/18/10144 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 18, p 10144
allfields_unstemmed	10.3390/ijms221810144 doi (DE-627)DOAJ071498192 (DE-599)DOAJe5b92328a8b04109b27b8bacc43aaccb DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Fahd Alhamdan verfasserin aut Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches. low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry Leigh M. Marsh verfasserin aut Frauke Pedersen verfasserin aut Bilal Alashkar Alhamwe verfasserin aut Clemens Thölken verfasserin aut Petra Ina Pfefferle verfasserin aut Thomas Bahmer verfasserin aut Timm Greulich verfasserin aut Daniel P. Potaczek verfasserin aut Holger Garn verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 18, p 10144 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:18, p 10144 https://doi.org/10.3390/ijms221810144 kostenfrei https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb kostenfrei https://www.mdpi.com/1422-0067/22/18/10144 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 18, p 10144
allfieldsGer	10.3390/ijms221810144 doi (DE-627)DOAJ071498192 (DE-599)DOAJe5b92328a8b04109b27b8bacc43aaccb DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Fahd Alhamdan verfasserin aut Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches. low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry Leigh M. Marsh verfasserin aut Frauke Pedersen verfasserin aut Bilal Alashkar Alhamwe verfasserin aut Clemens Thölken verfasserin aut Petra Ina Pfefferle verfasserin aut Thomas Bahmer verfasserin aut Timm Greulich verfasserin aut Daniel P. Potaczek verfasserin aut Holger Garn verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 18, p 10144 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:18, p 10144 https://doi.org/10.3390/ijms221810144 kostenfrei https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb kostenfrei https://www.mdpi.com/1422-0067/22/18/10144 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 18, p 10144
allfieldsSound	10.3390/ijms221810144 doi (DE-627)DOAJ071498192 (DE-599)DOAJe5b92328a8b04109b27b8bacc43aaccb DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Fahd Alhamdan verfasserin aut Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches. low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry Leigh M. Marsh verfasserin aut Frauke Pedersen verfasserin aut Bilal Alashkar Alhamwe verfasserin aut Clemens Thölken verfasserin aut Petra Ina Pfefferle verfasserin aut Thomas Bahmer verfasserin aut Timm Greulich verfasserin aut Daniel P. Potaczek verfasserin aut Holger Garn verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 18, p 10144 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:18, p 10144 https://doi.org/10.3390/ijms221810144 kostenfrei https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb kostenfrei https://www.mdpi.com/1422-0067/22/18/10144 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 18, p 10144
language	English
source	In International Journal of Molecular Sciences 22(2021), 18, p 10144 volume:22 year:2021 number:18, p 10144
sourceStr	In International Journal of Molecular Sciences 22(2021), 18, p 10144 volume:22 year:2021 number:18, p 10144
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Fahd Alhamdan @@aut@@ Leigh M. Marsh @@aut@@ Frauke Pedersen @@aut@@ Bilal Alashkar Alhamwe @@aut@@ Clemens Thölken @@aut@@ Petra Ina Pfefferle @@aut@@ Thomas Bahmer @@aut@@ Timm Greulich @@aut@@ Daniel P. Potaczek @@aut@@ Holger Garn @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ071498192
language_de	englisch
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callnumber-first	Q - Science
author	Fahd Alhamdan
spellingShingle	Fahd Alhamdan misc QH301-705.5 misc QD1-999 misc low type-2 asthma misc obesity misc asthma phenotypes misc CD4<sup<+</sup< T cells misc transcriptomics misc Biology (General) misc Chemistry Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype
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topic_title	QH301-705.5 QD1-999 Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype low type-2 asthma obesity asthma phenotypes CD4<sup<+</sup< T cells transcriptomics
topic	misc QH301-705.5 misc QD1-999 misc low type-2 asthma misc obesity misc asthma phenotypes misc CD4<sup<+</sup< T cells misc transcriptomics misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc low type-2 asthma misc obesity misc asthma phenotypes misc CD4<sup<+</sup< T cells misc transcriptomics misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc low type-2 asthma misc obesity misc asthma phenotypes misc CD4<sup<+</sup< T cells misc transcriptomics misc Biology (General) misc Chemistry
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title	Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype
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title_full	Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype
author_sort	Fahd Alhamdan
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author_browse	Fahd Alhamdan Leigh M. Marsh Frauke Pedersen Bilal Alashkar Alhamwe Clemens Thölken Petra Ina Pfefferle Thomas Bahmer Timm Greulich Daniel P. Potaczek Holger Garn
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doi_str_mv	10.3390/ijms221810144
author2-role	verfasserin
title_sort	differential regulation of interferon signaling pathways in cd4<sup<+</sup< t cells of the low type-2 obesity-associated asthma phenotype
callnumber	QH301-705.5
title_auth	Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype
abstract	In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches.
abstractGer	In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches.
abstract_unstemmed	In the era of personalized medicine, insights into the molecular mechanisms that differentially contribute to disease phenotypes, such as asthma phenotypes including obesity-associated asthma, are urgently needed. Peripheral blood was drawn from 10 obese, non-atopic asthmatic adults with a high body mass index (BMI; 36.67 ± 6.90); 10 non-obese, non-atopic asthmatic adults with normal BMI (23.88 ± 2.73); and 10 healthy controls with normal BMI (23.62 ± 3.74). All asthmatic patients were considered to represent a low type-2 asthma phenotype according to selective clinical parameters. RNA sequencing (RNA-Seq) was conducted on peripheral blood CD4<sup<+</sup< T cells. Thousands of differentially expressed genes were identified in both asthma groups compared with heathy controls. The expression of interferon (IFN)-stimulated genes associated with IFN-related signaling pathways was specifically affected in obese asthmatics, while the gap junction and G protein-coupled receptor (GPCR) ligand binding pathways were enriched in both asthma groups. Furthermore, obesity gene markers were also upregulated in CD4<sup<+</sup< T cells from obese asthmatics compared with the two other groups. Additionally, the enriched genes of the three abovementioned pathways showed a unique correlation pattern with various laboratory and clinical parameters. The specific activation of IFN-related signaling and viral infection pathways might provide a novel view of the molecular mechanisms associated with the development of the low type-2 obesity-associated asthma phenotype, which is a step ahead in the development of new stratified therapeutic approaches.
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title_short	Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype
url	https://doi.org/10.3390/ijms221810144 https://doaj.org/article/e5b92328a8b04109b27b8bacc43aaccb https://www.mdpi.com/1422-0067/22/18/10144 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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Differential Regulation of Interferon Signaling Pathways in CD4<sup<+</sup< T Cells of the Low Type-2 Obesity-Associated Asthma Phenotype

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