Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells

Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to gen...
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Autor*in:	Etienne-Pascal Journet [verfasserIn] Naima El-Gachtouli [verfasserIn] Vanessa Vernoud [verfasserIn] Françoise de Billy [verfasserIn] Magalie Pichon [verfasserIn] Annie Dedieu [verfasserIn] Christine Arnould [verfasserIn] Dominique Morandi [verfasserIn] David G. Barker [verfasserIn] Vivienne Gianinazzi-Pearson [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2001

Schlagwörter:	Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells

Übergeordnetes Werk:	In: Molecular Plant-Microbe Interactions - The American Phytopathological Society, 2021, 14(2001), 6, Seite 737-748
Übergeordnetes Werk:	volume:14 ; year:2001 ; number:6 ; pages:737-748

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1094/MPMI.2001.14.6.737

Katalog-ID:	DOAJ071608621

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520			\|a Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed.
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allfields	10.1094/MPMI.2001.14.6.737 doi (DE-627)DOAJ071608621 (DE-599)DOAJace6a782469646dbad963b81f36144f3 DE-627 ger DE-627 rakwb eng QR1-502 QK1-989 Etienne-Pascal Journet verfasserin aut Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed. Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells Microbiology Botany Naima El-Gachtouli verfasserin aut Vanessa Vernoud verfasserin aut Françoise de Billy verfasserin aut Magalie Pichon verfasserin aut Annie Dedieu verfasserin aut Christine Arnould verfasserin aut Dominique Morandi verfasserin aut David G. Barker verfasserin aut Vivienne Gianinazzi-Pearson verfasserin aut In Molecular Plant-Microbe Interactions The American Phytopathological Society, 2021 14(2001), 6, Seite 737-748 (DE-627)325568367 (DE-600)2037108-1 19437706 nnns volume:14 year:2001 number:6 pages:737-748 https://doi.org/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/article/ace6a782469646dbad963b81f36144f3 kostenfrei https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/toc/0894-0282 Journal toc kostenfrei https://doaj.org/toc/1943-7706 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2001 6 737-748
spelling	10.1094/MPMI.2001.14.6.737 doi (DE-627)DOAJ071608621 (DE-599)DOAJace6a782469646dbad963b81f36144f3 DE-627 ger DE-627 rakwb eng QR1-502 QK1-989 Etienne-Pascal Journet verfasserin aut Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed. Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells Microbiology Botany Naima El-Gachtouli verfasserin aut Vanessa Vernoud verfasserin aut Françoise de Billy verfasserin aut Magalie Pichon verfasserin aut Annie Dedieu verfasserin aut Christine Arnould verfasserin aut Dominique Morandi verfasserin aut David G. Barker verfasserin aut Vivienne Gianinazzi-Pearson verfasserin aut In Molecular Plant-Microbe Interactions The American Phytopathological Society, 2021 14(2001), 6, Seite 737-748 (DE-627)325568367 (DE-600)2037108-1 19437706 nnns volume:14 year:2001 number:6 pages:737-748 https://doi.org/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/article/ace6a782469646dbad963b81f36144f3 kostenfrei https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/toc/0894-0282 Journal toc kostenfrei https://doaj.org/toc/1943-7706 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2001 6 737-748
allfields_unstemmed	10.1094/MPMI.2001.14.6.737 doi (DE-627)DOAJ071608621 (DE-599)DOAJace6a782469646dbad963b81f36144f3 DE-627 ger DE-627 rakwb eng QR1-502 QK1-989 Etienne-Pascal Journet verfasserin aut Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed. Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells Microbiology Botany Naima El-Gachtouli verfasserin aut Vanessa Vernoud verfasserin aut Françoise de Billy verfasserin aut Magalie Pichon verfasserin aut Annie Dedieu verfasserin aut Christine Arnould verfasserin aut Dominique Morandi verfasserin aut David G. Barker verfasserin aut Vivienne Gianinazzi-Pearson verfasserin aut In Molecular Plant-Microbe Interactions The American Phytopathological Society, 2021 14(2001), 6, Seite 737-748 (DE-627)325568367 (DE-600)2037108-1 19437706 nnns volume:14 year:2001 number:6 pages:737-748 https://doi.org/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/article/ace6a782469646dbad963b81f36144f3 kostenfrei https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/toc/0894-0282 Journal toc kostenfrei https://doaj.org/toc/1943-7706 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2001 6 737-748
allfieldsGer	10.1094/MPMI.2001.14.6.737 doi (DE-627)DOAJ071608621 (DE-599)DOAJace6a782469646dbad963b81f36144f3 DE-627 ger DE-627 rakwb eng QR1-502 QK1-989 Etienne-Pascal Journet verfasserin aut Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed. Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells Microbiology Botany Naima El-Gachtouli verfasserin aut Vanessa Vernoud verfasserin aut Françoise de Billy verfasserin aut Magalie Pichon verfasserin aut Annie Dedieu verfasserin aut Christine Arnould verfasserin aut Dominique Morandi verfasserin aut David G. Barker verfasserin aut Vivienne Gianinazzi-Pearson verfasserin aut In Molecular Plant-Microbe Interactions The American Phytopathological Society, 2021 14(2001), 6, Seite 737-748 (DE-627)325568367 (DE-600)2037108-1 19437706 nnns volume:14 year:2001 number:6 pages:737-748 https://doi.org/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/article/ace6a782469646dbad963b81f36144f3 kostenfrei https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/toc/0894-0282 Journal toc kostenfrei https://doaj.org/toc/1943-7706 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2001 6 737-748
allfieldsSound	10.1094/MPMI.2001.14.6.737 doi (DE-627)DOAJ071608621 (DE-599)DOAJace6a782469646dbad963b81f36144f3 DE-627 ger DE-627 rakwb eng QR1-502 QK1-989 Etienne-Pascal Journet verfasserin aut Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells 2001 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed. Glomus spp. in situ hybridization Sinorhizobium meliloti transfer cells Microbiology Botany Naima El-Gachtouli verfasserin aut Vanessa Vernoud verfasserin aut Françoise de Billy verfasserin aut Magalie Pichon verfasserin aut Annie Dedieu verfasserin aut Christine Arnould verfasserin aut Dominique Morandi verfasserin aut David G. Barker verfasserin aut Vivienne Gianinazzi-Pearson verfasserin aut In Molecular Plant-Microbe Interactions The American Phytopathological Society, 2021 14(2001), 6, Seite 737-748 (DE-627)325568367 (DE-600)2037108-1 19437706 nnns volume:14 year:2001 number:6 pages:737-748 https://doi.org/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/article/ace6a782469646dbad963b81f36144f3 kostenfrei https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 kostenfrei https://doaj.org/toc/0894-0282 Journal toc kostenfrei https://doaj.org/toc/1943-7706 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 14 2001 6 737-748
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spellingShingle	Etienne-Pascal Journet misc QR1-502 misc QK1-989 misc Glomus spp. misc in situ hybridization misc Sinorhizobium meliloti misc transfer cells misc Microbiology misc Botany Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells
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issn	19437706
topic_title	QR1-502 QK1-989 Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells Glomus spp in situ hybridization Sinorhizobium meliloti transfer cells
topic	misc QR1-502 misc QK1-989 misc Glomus spp. misc in situ hybridization misc Sinorhizobium meliloti misc transfer cells misc Microbiology misc Botany
topic_unstemmed	misc QR1-502 misc QK1-989 misc Glomus spp. misc in situ hybridization misc Sinorhizobium meliloti misc transfer cells misc Microbiology misc Botany
topic_browse	misc QR1-502 misc QK1-989 misc Glomus spp. misc in situ hybridization misc Sinorhizobium meliloti misc transfer cells misc Microbiology misc Botany
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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hierarchy_parent_title	Molecular Plant-Microbe Interactions
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title	Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells
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title_full	Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells
author_sort	Etienne-Pascal Journet
journal	Molecular Plant-Microbe Interactions
journalStr	Molecular Plant-Microbe Interactions
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author_browse	Etienne-Pascal Journet Naima El-Gachtouli Vanessa Vernoud Françoise de Billy Magalie Pichon Annie Dedieu Christine Arnould Dominique Morandi David G. Barker Vivienne Gianinazzi-Pearson
container_volume	14
class	QR1-502 QK1-989
format_se	Elektronische Aufsätze
author-letter	Etienne-Pascal Journet
doi_str_mv	10.1094/MPMI.2001.14.6.737
author2-role	verfasserin
title_sort	medicago truncatula enod11: a novel rprp-encoding early nodulin gene expressed during mycorrhization in arbuscule-containing cells
callnumber	QR1-502
title_auth	Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells
abstract	Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed.
abstractGer	Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed.
abstract_unstemmed	Leguminous plants establish endosymbiotic associations with both rhizobia (nitrogen fixation) and arbuscular mycorrhizal fungi (phosphate uptake). These associations involve controlled entry of the soil microsymbiont into the root and the coordinated differentiation of the respective partners to generate the appropriate exchange interfaces. As part of a study to evaluate analogies at the molecular level between these two plant-microbe interactions, we focused on genes from Medicago truncatula encoding putative cell wall repetitive proline-rich proteins (RPRPs) expressed during the early stages of root nodulation. Here we report that a novel RPRP-encoding gene, MtENOD11, is transcribed during preinfection and infection stages of nodulation in root and nodule tissues. By means of reverse transcription-polymerase chain reaction and a promoter-reporter gene strategy, we demonstrate that this gene is also expressed during root colonization by endomycorrhizal fungi in inner cortical cells containing recently formed arbuscules. In contrast, no activation of MtENOD11 is observed during root colonization by a nonsymbiotic, biotrophic Rhizoctonia fungal species. Analysis of transgenic Medicago spp. plants expressing pMtENOD11-gusA also revealed that this gene is transcribed in a variety of nonsymbiotic specialized cell types in the root, shoot, and developing seed, either sharing high secretion/metabolite exchange activity or subject to regulated modifications in cell shape. The potential role of early nodulins with atypical RPRP structures such as ENOD11 and ENOD12 in symbiotic and nonsymbiotic cellular contexts is discussed.
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container_issue	6
title_short	Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing Cells
url	https://doi.org/10.1094/MPMI.2001.14.6.737 https://doaj.org/article/ace6a782469646dbad963b81f36144f3 https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.6.737 https://doaj.org/toc/0894-0282 https://doaj.org/toc/1943-7706
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author2	Naima El-Gachtouli Vanessa Vernoud Françoise de Billy Magalie Pichon Annie Dedieu Christine Arnould Dominique Morandi David G. Barker Vivienne Gianinazzi-Pearson
author2Str	Naima El-Gachtouli Vanessa Vernoud Françoise de Billy Magalie Pichon Annie Dedieu Christine Arnould Dominique Morandi David G. Barker Vivienne Gianinazzi-Pearson
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up_date	2024-07-03T21:12:48.410Z
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