Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition

Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jia Zhou [verfasserIn] Shuangming Yue [verfasserIn] Benchu Xue [verfasserIn] Zhisheng Wang [verfasserIn] Lizhi Wang [verfasserIn] Quanhui Peng [verfasserIn] Bai Xue [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	hyperthermia protein synthesis methionine cellular mechanism

Übergeordnetes Werk:	In: Journal of Animal Science and Technology - Korean Society of Animal Sciences and Technology, 2014, 63(2021), 5, Seite 1126-1141
Übergeordnetes Werk:	volume:63 ; year:2021 ; number:5 ; pages:1126-1141

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.5187/jast.2021.e93

Katalog-ID:	DOAJ071850481

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520			\|a Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.
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allfields	10.5187/jast.2021.e93 doi (DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2 DE-627 ger DE-627 rakwb eng SF1-1100 Jia Zhou verfasserin aut Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. hyperthermia protein synthesis methionine cellular mechanism Animal culture Shuangming Yue verfasserin aut Benchu Xue verfasserin aut Zhisheng Wang verfasserin aut Lizhi Wang verfasserin aut Quanhui Peng verfasserin aut Bai Xue verfasserin aut In Journal of Animal Science and Technology Korean Society of Animal Sciences and Technology, 2014 63(2021), 5, Seite 1126-1141 (DE-627)788845659 (DE-600)2775231-8 20550391 nnns volume:63 year:2021 number:5 pages:1126-1141 https://doi.org/10.5187/jast.2021.e93 kostenfrei https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 kostenfrei http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 kostenfrei https://doaj.org/toc/2672-0191 Journal toc kostenfrei https://doaj.org/toc/2055-0391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 63 2021 5 1126-1141
spelling	10.5187/jast.2021.e93 doi (DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2 DE-627 ger DE-627 rakwb eng SF1-1100 Jia Zhou verfasserin aut Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. hyperthermia protein synthesis methionine cellular mechanism Animal culture Shuangming Yue verfasserin aut Benchu Xue verfasserin aut Zhisheng Wang verfasserin aut Lizhi Wang verfasserin aut Quanhui Peng verfasserin aut Bai Xue verfasserin aut In Journal of Animal Science and Technology Korean Society of Animal Sciences and Technology, 2014 63(2021), 5, Seite 1126-1141 (DE-627)788845659 (DE-600)2775231-8 20550391 nnns volume:63 year:2021 number:5 pages:1126-1141 https://doi.org/10.5187/jast.2021.e93 kostenfrei https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 kostenfrei http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 kostenfrei https://doaj.org/toc/2672-0191 Journal toc kostenfrei https://doaj.org/toc/2055-0391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 63 2021 5 1126-1141
allfields_unstemmed	10.5187/jast.2021.e93 doi (DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2 DE-627 ger DE-627 rakwb eng SF1-1100 Jia Zhou verfasserin aut Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. hyperthermia protein synthesis methionine cellular mechanism Animal culture Shuangming Yue verfasserin aut Benchu Xue verfasserin aut Zhisheng Wang verfasserin aut Lizhi Wang verfasserin aut Quanhui Peng verfasserin aut Bai Xue verfasserin aut In Journal of Animal Science and Technology Korean Society of Animal Sciences and Technology, 2014 63(2021), 5, Seite 1126-1141 (DE-627)788845659 (DE-600)2775231-8 20550391 nnns volume:63 year:2021 number:5 pages:1126-1141 https://doi.org/10.5187/jast.2021.e93 kostenfrei https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 kostenfrei http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 kostenfrei https://doaj.org/toc/2672-0191 Journal toc kostenfrei https://doaj.org/toc/2055-0391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 63 2021 5 1126-1141
allfieldsGer	10.5187/jast.2021.e93 doi (DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2 DE-627 ger DE-627 rakwb eng SF1-1100 Jia Zhou verfasserin aut Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. hyperthermia protein synthesis methionine cellular mechanism Animal culture Shuangming Yue verfasserin aut Benchu Xue verfasserin aut Zhisheng Wang verfasserin aut Lizhi Wang verfasserin aut Quanhui Peng verfasserin aut Bai Xue verfasserin aut In Journal of Animal Science and Technology Korean Society of Animal Sciences and Technology, 2014 63(2021), 5, Seite 1126-1141 (DE-627)788845659 (DE-600)2775231-8 20550391 nnns volume:63 year:2021 number:5 pages:1126-1141 https://doi.org/10.5187/jast.2021.e93 kostenfrei https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 kostenfrei http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 kostenfrei https://doaj.org/toc/2672-0191 Journal toc kostenfrei https://doaj.org/toc/2055-0391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 63 2021 5 1126-1141
allfieldsSound	10.5187/jast.2021.e93 doi (DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2 DE-627 ger DE-627 rakwb eng SF1-1100 Jia Zhou verfasserin aut Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells. hyperthermia protein synthesis methionine cellular mechanism Animal culture Shuangming Yue verfasserin aut Benchu Xue verfasserin aut Zhisheng Wang verfasserin aut Lizhi Wang verfasserin aut Quanhui Peng verfasserin aut Bai Xue verfasserin aut In Journal of Animal Science and Technology Korean Society of Animal Sciences and Technology, 2014 63(2021), 5, Seite 1126-1141 (DE-627)788845659 (DE-600)2775231-8 20550391 nnns volume:63 year:2021 number:5 pages:1126-1141 https://doi.org/10.5187/jast.2021.e93 kostenfrei https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 kostenfrei http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 kostenfrei https://doaj.org/toc/2672-0191 Journal toc kostenfrei https://doaj.org/toc/2055-0391 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 63 2021 5 1126-1141
language	English
source	In Journal of Animal Science and Technology 63(2021), 5, Seite 1126-1141 volume:63 year:2021 number:5 pages:1126-1141
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topic_facet	hyperthermia protein synthesis methionine cellular mechanism Animal culture
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authorswithroles_txt_mv	Jia Zhou @@aut@@ Shuangming Yue @@aut@@ Benchu Xue @@aut@@ Zhisheng Wang @@aut@@ Lizhi Wang @@aut@@ Quanhui Peng @@aut@@ Bai Xue @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
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topic_title	SF1-1100 Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition hyperthermia protein synthesis methionine cellular mechanism
topic	misc SF1-1100 misc hyperthermia misc protein synthesis misc methionine misc cellular mechanism misc Animal culture
topic_unstemmed	misc SF1-1100 misc hyperthermia misc protein synthesis misc methionine misc cellular mechanism misc Animal culture
topic_browse	misc SF1-1100 misc hyperthermia misc protein synthesis misc methionine misc cellular mechanism misc Animal culture
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title	Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
ctrlnum	(DE-627)DOAJ071850481 (DE-599)DOAJ29f7ec8d97c54578af18415dab287aa2
title_full	Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
author_sort	Jia Zhou
journal	Journal of Animal Science and Technology
journalStr	Journal of Animal Science and Technology
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author_browse	Jia Zhou Shuangming Yue Benchu Xue Zhisheng Wang Lizhi Wang Quanhui Peng Bai Xue
container_volume	63
class	SF1-1100
format_se	Elektronische Aufsätze
author-letter	Jia Zhou
doi_str_mv	10.5187/jast.2021.e93
author2-role	verfasserin
title_sort	enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
callnumber	SF1-1100
title_auth	Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
abstract	Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.
abstractGer	Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.
abstract_unstemmed	Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42°C for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 μg/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 μg/mL (MET70, 70 μg/mL of Met), 20 μg/mL (MET80, 80 μg/mL of Met), and 30 μg/mL (MET90,90 μg/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 μg/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 μg/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 μg/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.
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container_issue	5
title_short	Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition
url	https://doi.org/10.5187/jast.2021.e93 https://doaj.org/article/29f7ec8d97c54578af18415dab287aa2 http://www.ejast.org/archive/view_article?pid=jast-63-5-1126 https://doaj.org/toc/2672-0191 https://doaj.org/toc/2055-0391
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author2	Shuangming Yue Benchu Xue Zhisheng Wang Lizhi Wang Quanhui Peng Bai Xue
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up_date	2024-07-03T22:31:00.225Z
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