Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model

<i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence...
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Autor*in:	Kar Yan Soh [verfasserIn] Jacelyn Mei San Loh [verfasserIn] Christopher Hall [verfasserIn] Thomas Proft [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	nuclease SpnAi nucleotidase S5nAi virulence

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 8(2020), 9, p 1361
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:9, p 1361

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms8091361

Katalog-ID:	DOAJ071861327

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520			\|a <i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent <i<S. iniae spnAi</i< and <i<s5nAi</i< deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in <i<S. iniae</i< could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) <i<S. iniae</i<. Deletion of <i<spnAi</i< and <i<s5nAi</i< resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in <i<S. iniae</i< immune evasion and might present potential targets for the development of therapeutic agents or vaccines.
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allfieldsSound	10.3390/microorganisms8091361 doi (DE-627)DOAJ071861327 (DE-599)DOAJefc59918417a4252841db9c59e683642 DE-627 ger DE-627 rakwb eng QH301-705.5 Kar Yan Soh verfasserin aut Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent <i<S. iniae spnAi</i< and <i<s5nAi</i< deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in <i<S. iniae</i< could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) <i<S. iniae</i<. Deletion of <i<spnAi</i< and <i<s5nAi</i< resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in <i<S. iniae</i< immune evasion and might present potential targets for the development of therapeutic agents or vaccines. nuclease SpnAi nucleotidase S5nAi <i<Streptococcus iniae</i< virulence Biology (General) Jacelyn Mei San Loh verfasserin aut Christopher Hall verfasserin aut Thomas Proft verfasserin aut In Microorganisms MDPI AG, 2013 8(2020), 9, p 1361 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:8 year:2020 number:9, p 1361 https://doi.org/10.3390/microorganisms8091361 kostenfrei https://doaj.org/article/efc59918417a4252841db9c59e683642 kostenfrei https://www.mdpi.com/2076-2607/8/9/1361 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 9, p 1361
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source	In Microorganisms 8(2020), 9, p 1361 volume:8 year:2020 number:9, p 1361
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authorswithroles_txt_mv	Kar Yan Soh @@aut@@ Jacelyn Mei San Loh @@aut@@ Christopher Hall @@aut@@ Thomas Proft @@aut@@
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callnumber-first	Q - Science
author	Kar Yan Soh
spellingShingle	Kar Yan Soh misc QH301-705.5 misc nuclease misc SpnAi misc nucleotidase misc S5nAi misc <i<Streptococcus iniae</i< misc virulence misc Biology (General) Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model
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topic_title	QH301-705.5 Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model nuclease SpnAi nucleotidase S5nAi <i<Streptococcus iniae</i< virulence
topic	misc QH301-705.5 misc nuclease misc SpnAi misc nucleotidase misc S5nAi misc <i<Streptococcus iniae</i< misc virulence misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc nuclease misc SpnAi misc nucleotidase misc S5nAi misc <i<Streptococcus iniae</i< misc virulence misc Biology (General)
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title	Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model
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title_full	Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model
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author_browse	Kar Yan Soh Jacelyn Mei San Loh Christopher Hall Thomas Proft
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title_sort	functional analysis of two novel <i<streptococcus iniae</i< virulence factors using a zebrafish infection model
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title_auth	Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model
abstract	<i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent <i<S. iniae spnAi</i< and <i<s5nAi</i< deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in <i<S. iniae</i< could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) <i<S. iniae</i<. Deletion of <i<spnAi</i< and <i<s5nAi</i< resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in <i<S. iniae</i< immune evasion and might present potential targets for the development of therapeutic agents or vaccines.
abstractGer	<i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent <i<S. iniae spnAi</i< and <i<s5nAi</i< deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in <i<S. iniae</i< could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) <i<S. iniae</i<. Deletion of <i<spnAi</i< and <i<s5nAi</i< resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in <i<S. iniae</i< immune evasion and might present potential targets for the development of therapeutic agents or vaccines.
abstract_unstemmed	<i<Streptococcus iniae</i< is a major fish pathogen that contributes to large annual losses in the aquaculture industry, exceeding US$100 million. It is also reported to cause opportunistic infections in humans. We have recently identified two novel <i<S. iniae</i< virulence factors, an extracellular nuclease (SpnAi) and a secreted nucleotidase (S5nAi), and verified their predicted enzymatic activities using recombinant proteins. Here, we report the generation of green fluorescent <i<S. iniae spnAi</i< and <i<s5nAi</i< deletion mutants and their evaluation in a transgenic zebrafish infection model. Our results show nuclease and nucleotidase activities in <i<S. iniae</i< could be attributed to SpnAi and S5nAi, respectively. Consistent with this, larvae infected with the deletion mutants demonstrated enhanced survival and bacterial clearance, compared to those infected with wild-type (WT) <i<S. iniae</i<. Deletion of <i<spnAi</i< and <i<s5nAi</i< resulted in sustained recruitment of neutrophils and macrophages, respectively, to the site of infection. We also show that recombinant SpnAi is able to degrade neutrophil extracellular traps (NETs) isolated from zebrafish kidney tissue. Our results suggest that both enzymes play an important role in <i<S. iniae</i< immune evasion and might present potential targets for the development of therapeutic agents or vaccines.
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container_issue	9, p 1361
title_short	Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model
url	https://doi.org/10.3390/microorganisms8091361 https://doaj.org/article/efc59918417a4252841db9c59e683642 https://www.mdpi.com/2076-2607/8/9/1361 https://doaj.org/toc/2076-2607
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Functional Analysis of Two Novel <i<Streptococcus iniae</i< Virulence Factors Using a Zebrafish Infection Model

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