Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells

To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total p...
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Autor*in:	Hye Ju Han [verfasserIn] Seon Kyeong Park [verfasserIn] Jin Yong Kang [verfasserIn] Jong Min Kim [verfasserIn] Seul Ki Yoo [verfasserIn] Ho Jin Heo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine

Übergeordnetes Werk:	In: Nutrients - MDPI AG, 2009, 12(2020), 3, p 832
Übergeordnetes Werk:	volume:12 ; year:2020 ; number:3, p 832

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nu12030832

Katalog-ID:	DOAJ071998152

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allfields	10.3390/nu12030832 doi (DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af DE-627 ger DE-627 rakwb eng TX341-641 Hye Ju Han verfasserin aut Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material. anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply Seon Kyeong Park verfasserin aut Jin Yong Kang verfasserin aut Jong Min Kim verfasserin aut Seul Ki Yoo verfasserin aut Ho Jin Heo verfasserin aut In Nutrients MDPI AG, 2009 12(2020), 3, p 832 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:12 year:2020 number:3, p 832 https://doi.org/10.3390/nu12030832 kostenfrei https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af kostenfrei https://www.mdpi.com/2072-6643/12/3/832 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 3, p 832
spelling	10.3390/nu12030832 doi (DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af DE-627 ger DE-627 rakwb eng TX341-641 Hye Ju Han verfasserin aut Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material. anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply Seon Kyeong Park verfasserin aut Jin Yong Kang verfasserin aut Jong Min Kim verfasserin aut Seul Ki Yoo verfasserin aut Ho Jin Heo verfasserin aut In Nutrients MDPI AG, 2009 12(2020), 3, p 832 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:12 year:2020 number:3, p 832 https://doi.org/10.3390/nu12030832 kostenfrei https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af kostenfrei https://www.mdpi.com/2072-6643/12/3/832 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 3, p 832
allfields_unstemmed	10.3390/nu12030832 doi (DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af DE-627 ger DE-627 rakwb eng TX341-641 Hye Ju Han verfasserin aut Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material. anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply Seon Kyeong Park verfasserin aut Jin Yong Kang verfasserin aut Jong Min Kim verfasserin aut Seul Ki Yoo verfasserin aut Ho Jin Heo verfasserin aut In Nutrients MDPI AG, 2009 12(2020), 3, p 832 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:12 year:2020 number:3, p 832 https://doi.org/10.3390/nu12030832 kostenfrei https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af kostenfrei https://www.mdpi.com/2072-6643/12/3/832 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 3, p 832
allfieldsGer	10.3390/nu12030832 doi (DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af DE-627 ger DE-627 rakwb eng TX341-641 Hye Ju Han verfasserin aut Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material. anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply Seon Kyeong Park verfasserin aut Jin Yong Kang verfasserin aut Jong Min Kim verfasserin aut Seul Ki Yoo verfasserin aut Ho Jin Heo verfasserin aut In Nutrients MDPI AG, 2009 12(2020), 3, p 832 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:12 year:2020 number:3, p 832 https://doi.org/10.3390/nu12030832 kostenfrei https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af kostenfrei https://www.mdpi.com/2072-6643/12/3/832 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 3, p 832
allfieldsSound	10.3390/nu12030832 doi (DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af DE-627 ger DE-627 rakwb eng TX341-641 Hye Ju Han verfasserin aut Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material. anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply Seon Kyeong Park verfasserin aut Jin Yong Kang verfasserin aut Jong Min Kim verfasserin aut Seul Ki Yoo verfasserin aut Ho Jin Heo verfasserin aut In Nutrients MDPI AG, 2009 12(2020), 3, p 832 (DE-627)610604155 (DE-600)2518386-2 20726643 nnns volume:12 year:2020 number:3, p 832 https://doi.org/10.3390/nu12030832 kostenfrei https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af kostenfrei https://www.mdpi.com/2072-6643/12/3/832 kostenfrei https://doaj.org/toc/2072-6643 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2020 3, p 832
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source	In Nutrients 12(2020), 3, p 832 volume:12 year:2020 number:3, p 832
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topic_facet	anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine Nutrition. Foods and food supply
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author	Hye Ju Han
spellingShingle	Hye Ju Han misc TX341-641 misc anti-melanogenesis misc b16/f10 melanoma cell misc hydroxyoctadecadienoic acid misc sorghum bicolor misc 3-isobutyl-1-methylxanthine misc Nutrition. Foods and food supply Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells
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topic_title	TX341-641 Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells anti-melanogenesis b16/f10 melanoma cell hydroxyoctadecadienoic acid sorghum bicolor 3-isobutyl-1-methylxanthine
topic	misc TX341-641 misc anti-melanogenesis misc b16/f10 melanoma cell misc hydroxyoctadecadienoic acid misc sorghum bicolor misc 3-isobutyl-1-methylxanthine misc Nutrition. Foods and food supply
topic_unstemmed	misc TX341-641 misc anti-melanogenesis misc b16/f10 melanoma cell misc hydroxyoctadecadienoic acid misc sorghum bicolor misc 3-isobutyl-1-methylxanthine misc Nutrition. Foods and food supply
topic_browse	misc TX341-641 misc anti-melanogenesis misc b16/f10 melanoma cell misc hydroxyoctadecadienoic acid misc sorghum bicolor misc 3-isobutyl-1-methylxanthine misc Nutrition. Foods and food supply
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title	Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells
ctrlnum	(DE-627)DOAJ071998152 (DE-599)DOAJ85edf37ab4844aebaa9c45af738d20af
title_full	Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells
author_sort	Hye Ju Han
journal	Nutrients
journalStr	Nutrients
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author_browse	Hye Ju Han Seon Kyeong Park Jin Yong Kang Jong Min Kim Seul Ki Yoo Ho Jin Heo
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class	TX341-641
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author-letter	Hye Ju Han
doi_str_mv	10.3390/nu12030832
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title_sort	anti-melanogenic effect of ethanolic extract of <i<sorghum bicolor</i< on ibmx–induced melanogenesis in b16/f10 melanoma cells
callnumber	TX341-641
title_auth	Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells
abstract	To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material.
abstractGer	To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material.
abstract_unstemmed	To evaluate possibility as a skin whitening agent of <i<Sorghum bicolor</i< (<i<S. bicolor</i<), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of <i<S. bicolor</i< (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2’-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC<sub<50</sub< value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3-<i<O</i<-dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS<sup<2</sup<). These findings suggest that ESB may have physiological potential to be used skin whitening material.
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container_issue	3, p 832
title_short	Anti-Melanogenic Effect of Ethanolic Extract of <i<Sorghum bicolor</i< on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells
url	https://doi.org/10.3390/nu12030832 https://doaj.org/article/85edf37ab4844aebaa9c45af738d20af https://www.mdpi.com/2072-6643/12/3/832 https://doaj.org/toc/2072-6643
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up_date	2024-07-03T23:15:22.196Z
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