The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers

Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Liwen Jiang [verfasserIn] Hongtao Nie [verfasserIn] Chen Li [verfasserIn] Dongdong Li [verfasserIn] Zhongming Huo [verfasserIn] Xiwu Yan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Übergeordnetes Werk:	In: Electronic Journal of Biotechnology - Elsevier, 2016, 34(2018), Seite 17-21
Übergeordnetes Werk:	volume:34 ; year:2018 ; pages:17-21

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.ejbt.2018.05.003

Katalog-ID:	DOAJ072011874

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allfields	10.1016/j.ejbt.2018.05.003 doi (DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Liwen Jiang verfasserin aut The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Biotechnology Biology (General) Hongtao Nie verfasserin aut Chen Li verfasserin aut Dongdong Li verfasserin aut Zhongming Huo verfasserin aut Xiwu Yan verfasserin aut In Electronic Journal of Biotechnology Elsevier, 2016 34(2018), Seite 17-21 (DE-627)320604713 (DE-600)2020598-3 07173458 nnns volume:34 year:2018 pages:17-21 https://doi.org/10.1016/j.ejbt.2018.05.003 kostenfrei https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d kostenfrei http://www.sciencedirect.com/science/article/pii/S0717345818300204 kostenfrei https://doaj.org/toc/0717-3458 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 34 2018 17-21
spelling	10.1016/j.ejbt.2018.05.003 doi (DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Liwen Jiang verfasserin aut The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Biotechnology Biology (General) Hongtao Nie verfasserin aut Chen Li verfasserin aut Dongdong Li verfasserin aut Zhongming Huo verfasserin aut Xiwu Yan verfasserin aut In Electronic Journal of Biotechnology Elsevier, 2016 34(2018), Seite 17-21 (DE-627)320604713 (DE-600)2020598-3 07173458 nnns volume:34 year:2018 pages:17-21 https://doi.org/10.1016/j.ejbt.2018.05.003 kostenfrei https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d kostenfrei http://www.sciencedirect.com/science/article/pii/S0717345818300204 kostenfrei https://doaj.org/toc/0717-3458 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 34 2018 17-21
allfields_unstemmed	10.1016/j.ejbt.2018.05.003 doi (DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Liwen Jiang verfasserin aut The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Biotechnology Biology (General) Hongtao Nie verfasserin aut Chen Li verfasserin aut Dongdong Li verfasserin aut Zhongming Huo verfasserin aut Xiwu Yan verfasserin aut In Electronic Journal of Biotechnology Elsevier, 2016 34(2018), Seite 17-21 (DE-627)320604713 (DE-600)2020598-3 07173458 nnns volume:34 year:2018 pages:17-21 https://doi.org/10.1016/j.ejbt.2018.05.003 kostenfrei https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d kostenfrei http://www.sciencedirect.com/science/article/pii/S0717345818300204 kostenfrei https://doaj.org/toc/0717-3458 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 34 2018 17-21
allfieldsGer	10.1016/j.ejbt.2018.05.003 doi (DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Liwen Jiang verfasserin aut The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Biotechnology Biology (General) Hongtao Nie verfasserin aut Chen Li verfasserin aut Dongdong Li verfasserin aut Zhongming Huo verfasserin aut Xiwu Yan verfasserin aut In Electronic Journal of Biotechnology Elsevier, 2016 34(2018), Seite 17-21 (DE-627)320604713 (DE-600)2020598-3 07173458 nnns volume:34 year:2018 pages:17-21 https://doi.org/10.1016/j.ejbt.2018.05.003 kostenfrei https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d kostenfrei http://www.sciencedirect.com/science/article/pii/S0717345818300204 kostenfrei https://doaj.org/toc/0717-3458 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 34 2018 17-21
allfieldsSound	10.1016/j.ejbt.2018.05.003 doi (DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH301-705.5 Liwen Jiang verfasserin aut The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat Biotechnology Biology (General) Hongtao Nie verfasserin aut Chen Li verfasserin aut Dongdong Li verfasserin aut Zhongming Huo verfasserin aut Xiwu Yan verfasserin aut In Electronic Journal of Biotechnology Elsevier, 2016 34(2018), Seite 17-21 (DE-627)320604713 (DE-600)2020598-3 07173458 nnns volume:34 year:2018 pages:17-21 https://doi.org/10.1016/j.ejbt.2018.05.003 kostenfrei https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d kostenfrei http://www.sciencedirect.com/science/article/pii/S0717345818300204 kostenfrei https://doaj.org/toc/0717-3458 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 34 2018 17-21
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topic_title	TP248.13-248.65 QH301-705.5 The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
topic	misc TP248.13-248.65 misc QH301-705.5 misc Biotechnology misc Biology (General)
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title	The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
ctrlnum	(DE-627)DOAJ072011874 (DE-599)DOAJ9e9e510f479641acaf96114b2a71b69d
title_full	The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
author_sort	Liwen Jiang
journal	Electronic Journal of Biotechnology
journalStr	Electronic Journal of Biotechnology
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container_start_page	17
author_browse	Liwen Jiang Hongtao Nie Chen Li Dongdong Li Zhongming Huo Xiwu Yan
container_volume	34
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format_se	Elektronische Aufsätze
author-letter	Liwen Jiang
doi_str_mv	10.1016/j.ejbt.2018.05.003
author2-role	verfasserin
title_sort	genetic diversity of wild and cultivated manila clam (ruditapes philippinarum) revealed by 29 novel microsatellite markers
callnumber	TP248.13-248.65
title_auth	The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
abstract	Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat
abstractGer	Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat
abstract_unstemmed	Background: Microsatellite loci often used as a genetic tool for estimating genetic diversity population variation in a wide variety of different species. The application of microsatellite markers in genetics and breeding includes investigating the genetic differentiation of wild and cultured populations, assessing and determining the genetic relationship of different populations. The aim of this work is to develop several microsatellite markers via high-throughput sequencing and characterize these markers in commercially important bivalve Ruditapes philippinarum. Results: Among the two populations of R. philippinarum studied, 110 alleles were detected. The number of alleles at the cultured population ranged from 3 to 17 (mean NA = 6.897) and wild population ranged from 2 to 15 (mean NA = 6.793). The observed and expected heterozygosities of cultured population ranged from 0.182 to 0.964, and from 0.286 to 0.900, with an average of 0.647 and 0.692, respectively. The observed and expected heterozygosities of wild population ranged from 0.138 to 1.000, and from 0.439 to 0.906, with an average of 0.674 and 0.693, respectively. The polymorphism information content ranged from 0.341 to 0.910 with an average of 0.687. Sixteen and thirteen microsatellite loci deviated significantly from Hardy–Weinberg equilibrium after correction for multiple tests in cultured and wild population, respectively. Conclusions: Twenty-nine novel microsatellite loci were developed using Illumina paired-end shotgun sequencing and characterized in two population of R. philippinarum.How to cite: Jiang L, Nie H, Li C, et al. The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers. Electron J Biotechnol 2018;34. https://doi.org/10.1016/j.ejbt.2018.05.003. Keywords: Aquaculture industry, Clam, Genetic diversity, High polymorphism, Illumina paired-end shotgun sequencing, Microsatellite, Novel microsatellite loci, Population genetics, Ruditapes philippinarum, Simple sequence repeat
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2119 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	The genetic diversity of wild and cultivated Manila clam (Ruditapes philippinarum) revealed by 29 novel microsatellite markers
url	https://doi.org/10.1016/j.ejbt.2018.05.003 https://doaj.org/article/9e9e510f479641acaf96114b2a71b69d http://www.sciencedirect.com/science/article/pii/S0717345818300204 https://doaj.org/toc/0717-3458
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up_date	2024-07-03T23:19:15.713Z
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