Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cell...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bakar R Abdel-Halim [verfasserIn] Walaa A Moselhy [verfasserIn] Nermeen Atef Helmy [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Asian Pacific Journal of Reproduction - Wolters Kluwer Medknow Publications, 2017, 7(2018), 4, Seite 161-166 |
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| Übergeordnetes Werk: |
volume:7 ; year:2018 ; number:4 ; pages:161-166 |
| Links: |
Link aufrufen |
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| DOI / URN: |
10.4103/2305-0500.237053 |
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| Katalog-ID: |
DOAJ072286393 |
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| 520 | |a Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. | ||
| 650 | 4 | |a Nanocopper-oxide particle | |
| 650 | 4 | |a Nanozinc-oxide particle | |
| 650 | 4 | |a Toxicity | |
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10.4103/2305-0500.237053 doi (DE-627)DOAJ072286393 (DE-599)DOAJaf441b654dc14bd1ac7f6c3329d4b09b DE-627 ger DE-627 rakwb eng Bakar R Abdel-Halim verfasserin aut Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle Medicine R Walaa A Moselhy verfasserin aut Nermeen Atef Helmy verfasserin aut In Asian Pacific Journal of Reproduction Wolters Kluwer Medknow Publications, 2017 7(2018), 4, Seite 161-166 (DE-627)775904678 (DE-600)2747542-6 23050519 nnns volume:7 year:2018 number:4 pages:161-166 https://doi.org/10.4103/2305-0500.237053 kostenfrei https://doaj.org/article/af441b654dc14bd1ac7f6c3329d4b09b kostenfrei http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=4;spage=161;epage=166;aulast=Abdel-Halim kostenfrei https://doaj.org/toc/2305-0500 Journal toc kostenfrei https://doaj.org/toc/2305-0519 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2004 GBV_ILN_2336 AR 7 2018 4 161-166 |
| spelling |
10.4103/2305-0500.237053 doi (DE-627)DOAJ072286393 (DE-599)DOAJaf441b654dc14bd1ac7f6c3329d4b09b DE-627 ger DE-627 rakwb eng Bakar R Abdel-Halim verfasserin aut Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle Medicine R Walaa A Moselhy verfasserin aut Nermeen Atef Helmy verfasserin aut In Asian Pacific Journal of Reproduction Wolters Kluwer Medknow Publications, 2017 7(2018), 4, Seite 161-166 (DE-627)775904678 (DE-600)2747542-6 23050519 nnns volume:7 year:2018 number:4 pages:161-166 https://doi.org/10.4103/2305-0500.237053 kostenfrei https://doaj.org/article/af441b654dc14bd1ac7f6c3329d4b09b kostenfrei http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=4;spage=161;epage=166;aulast=Abdel-Halim kostenfrei https://doaj.org/toc/2305-0500 Journal toc kostenfrei https://doaj.org/toc/2305-0519 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2004 GBV_ILN_2336 AR 7 2018 4 161-166 |
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10.4103/2305-0500.237053 doi (DE-627)DOAJ072286393 (DE-599)DOAJaf441b654dc14bd1ac7f6c3329d4b09b DE-627 ger DE-627 rakwb eng Bakar R Abdel-Halim verfasserin aut Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle Medicine R Walaa A Moselhy verfasserin aut Nermeen Atef Helmy verfasserin aut In Asian Pacific Journal of Reproduction Wolters Kluwer Medknow Publications, 2017 7(2018), 4, Seite 161-166 (DE-627)775904678 (DE-600)2747542-6 23050519 nnns volume:7 year:2018 number:4 pages:161-166 https://doi.org/10.4103/2305-0500.237053 kostenfrei https://doaj.org/article/af441b654dc14bd1ac7f6c3329d4b09b kostenfrei http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=4;spage=161;epage=166;aulast=Abdel-Halim kostenfrei https://doaj.org/toc/2305-0500 Journal toc kostenfrei https://doaj.org/toc/2305-0519 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2004 GBV_ILN_2336 AR 7 2018 4 161-166 |
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10.4103/2305-0500.237053 doi (DE-627)DOAJ072286393 (DE-599)DOAJaf441b654dc14bd1ac7f6c3329d4b09b DE-627 ger DE-627 rakwb eng Bakar R Abdel-Halim verfasserin aut Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle Medicine R Walaa A Moselhy verfasserin aut Nermeen Atef Helmy verfasserin aut In Asian Pacific Journal of Reproduction Wolters Kluwer Medknow Publications, 2017 7(2018), 4, Seite 161-166 (DE-627)775904678 (DE-600)2747542-6 23050519 nnns volume:7 year:2018 number:4 pages:161-166 https://doi.org/10.4103/2305-0500.237053 kostenfrei https://doaj.org/article/af441b654dc14bd1ac7f6c3329d4b09b kostenfrei http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=4;spage=161;epage=166;aulast=Abdel-Halim kostenfrei https://doaj.org/toc/2305-0500 Journal toc kostenfrei https://doaj.org/toc/2305-0519 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2004 GBV_ILN_2336 AR 7 2018 4 161-166 |
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10.4103/2305-0500.237053 doi (DE-627)DOAJ072286393 (DE-599)DOAJaf441b654dc14bd1ac7f6c3329d4b09b DE-627 ger DE-627 rakwb eng Bakar R Abdel-Halim verfasserin aut Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle Medicine R Walaa A Moselhy verfasserin aut Nermeen Atef Helmy verfasserin aut In Asian Pacific Journal of Reproduction Wolters Kluwer Medknow Publications, 2017 7(2018), 4, Seite 161-166 (DE-627)775904678 (DE-600)2747542-6 23050519 nnns volume:7 year:2018 number:4 pages:161-166 https://doi.org/10.4103/2305-0500.237053 kostenfrei https://doaj.org/article/af441b654dc14bd1ac7f6c3329d4b09b kostenfrei http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=4;spage=161;epage=166;aulast=Abdel-Halim kostenfrei https://doaj.org/toc/2305-0500 Journal toc kostenfrei https://doaj.org/toc/2305-0519 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_2004 GBV_ILN_2336 AR 7 2018 4 161-166 |
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Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. 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Bakar R Abdel-Halim |
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Bakar R Abdel-Halim misc Nanocopper-oxide particle misc Nanozinc-oxide particle misc Toxicity misc IVM misc In vitro embryo production misc Cattle misc Medicine misc R Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation |
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Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation Nanocopper-oxide particle Nanozinc-oxide particle Toxicity IVM In vitro embryo production Cattle |
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developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation |
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Developmental competence of bovine oocytes with increasing concentrations of nano-copper and nano-zinc particles during in vitro maturation |
| abstract |
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. |
| abstractGer |
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. |
| abstract_unstemmed |
Objective: To evaluate copper and zinc concentrations in plasma and follicular fluid from cattle ovaries, and estimate the impact of rational concentrations of copper and zinc oxide nanoparticles (CuO-NPs & ZnO-NPs) supplemented during in vitro maturation (IVM) against DNA damage of cumulus cells, glutathione content in oocytes and on consequent embryo development. Methods: Oocytes were obtained from 2 to 8 mm follicles by aspiration method for IVM. Replicates of experiments were performed on different days, with a separate batch of cumulus oocytes complex for each day. Results: The DNA damage of oocytes and cumulus cells significantly decreased with supplemental CuO-NPs or ZnO-NPs concentrations of 0.7 and 1.0 μg/mL in the IVM medium compared to medium without CuO-NPs or ZnO-NPs (P < 0.01). Total glutathione concentrations in oocytes and cumulus cells significantly increased following supplementation with both 0.7 and 1.0 μg/mL CuO-NPs or ZnO-NPs in comparison with 0 and 0.4 μg/mL CuO-NPs or ZnO-NPs supplemented groups (P < 0.01). Supplementation of CuO-NPs or ZnO-NPs during IVM medium at any concentration had no significant effect on cleavge rate. Both CuO-NPs and ZnO-NPs significantly increased blastocyst rates when oocytes were matured with 0.7, 1.0 μg/mL CuO-NPs concentrations (P < 0.01). In contrast, addition of 1.5 μg/mL of CuO-NPs or ZnO-NPs to the maturation media resulted in detrimental effects on the developmental competence of bovine oocytes confirming toxicity induced by CuO-NPs and ZnO-NPs in high concentrations. Conclusions: CuO-NPs or ZnO-NPs-treated bovine oocytes during IVM show low level of DNA fragmentation and increased intracellular glutathione content of cumulus cells. In vitro embryo development is improved by supplementation of rational concentrations of CuO-NPs or ZnO-NPs to culture media. Toxicity induced by CuO-NPs and ZnO-NPs is confirmed in high concentrations. |
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