Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens

In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only...
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Autor*in:	Pengsong Li [verfasserIn] Yumingzi Wang [verfasserIn] Xin Yuan [verfasserIn] Xinying Liu [verfasserIn] Chunmao Liu [verfasserIn] Xiaofen Fu [verfasserIn] Dezhi Sun [verfasserIn] Yan Dang [verfasserIn] Dawn E. Holmes [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon

Übergeordnetes Werk:	In: Environmental Science and Ecotechnology - Elsevier, 2021, 6(2021), Seite 100092-
Übergeordnetes Werk:	volume:6 ; year:2021 ; pages:100092-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.ese.2021.100092

Katalog-ID:	DOAJ072371277

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520			\|a In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.
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700	0		\|a Xiaofen Fu \|e verfasserin \|4 aut
700	0		\|a Dezhi Sun \|e verfasserin \|4 aut
700	0		\|a Yan Dang \|e verfasserin \|4 aut
700	0		\|a Dawn E. Holmes \|e verfasserin \|4 aut
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publishDate	2021
allfields	10.1016/j.ese.2021.100092 doi (DE-627)DOAJ072371277 (DE-599)DOAJ7415663cca38486394d992fc113738d5 DE-627 ger DE-627 rakwb eng GE1-350 TD1-1066 Pengsong Li verfasserin aut Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples. Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering Yumingzi Wang verfasserin aut Xin Yuan verfasserin aut Xinying Liu verfasserin aut Chunmao Liu verfasserin aut Xiaofen Fu verfasserin aut Dezhi Sun verfasserin aut Yan Dang verfasserin aut Dawn E. Holmes verfasserin aut In Environmental Science and Ecotechnology Elsevier, 2021 6(2021), Seite 100092- (DE-627)1747161268 26664984 nnns volume:6 year:2021 pages:100092- https://doi.org/10.1016/j.ese.2021.100092 kostenfrei https://doaj.org/article/7415663cca38486394d992fc113738d5 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666498421000156 kostenfrei https://doaj.org/toc/2666-4984 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 6 2021 100092-
spelling	10.1016/j.ese.2021.100092 doi (DE-627)DOAJ072371277 (DE-599)DOAJ7415663cca38486394d992fc113738d5 DE-627 ger DE-627 rakwb eng GE1-350 TD1-1066 Pengsong Li verfasserin aut Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples. Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering Yumingzi Wang verfasserin aut Xin Yuan verfasserin aut Xinying Liu verfasserin aut Chunmao Liu verfasserin aut Xiaofen Fu verfasserin aut Dezhi Sun verfasserin aut Yan Dang verfasserin aut Dawn E. Holmes verfasserin aut In Environmental Science and Ecotechnology Elsevier, 2021 6(2021), Seite 100092- (DE-627)1747161268 26664984 nnns volume:6 year:2021 pages:100092- https://doi.org/10.1016/j.ese.2021.100092 kostenfrei https://doaj.org/article/7415663cca38486394d992fc113738d5 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666498421000156 kostenfrei https://doaj.org/toc/2666-4984 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 6 2021 100092-
allfields_unstemmed	10.1016/j.ese.2021.100092 doi (DE-627)DOAJ072371277 (DE-599)DOAJ7415663cca38486394d992fc113738d5 DE-627 ger DE-627 rakwb eng GE1-350 TD1-1066 Pengsong Li verfasserin aut Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples. Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering Yumingzi Wang verfasserin aut Xin Yuan verfasserin aut Xinying Liu verfasserin aut Chunmao Liu verfasserin aut Xiaofen Fu verfasserin aut Dezhi Sun verfasserin aut Yan Dang verfasserin aut Dawn E. Holmes verfasserin aut In Environmental Science and Ecotechnology Elsevier, 2021 6(2021), Seite 100092- (DE-627)1747161268 26664984 nnns volume:6 year:2021 pages:100092- https://doi.org/10.1016/j.ese.2021.100092 kostenfrei https://doaj.org/article/7415663cca38486394d992fc113738d5 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666498421000156 kostenfrei https://doaj.org/toc/2666-4984 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 6 2021 100092-
allfieldsGer	10.1016/j.ese.2021.100092 doi (DE-627)DOAJ072371277 (DE-599)DOAJ7415663cca38486394d992fc113738d5 DE-627 ger DE-627 rakwb eng GE1-350 TD1-1066 Pengsong Li verfasserin aut Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples. Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering Yumingzi Wang verfasserin aut Xin Yuan verfasserin aut Xinying Liu verfasserin aut Chunmao Liu verfasserin aut Xiaofen Fu verfasserin aut Dezhi Sun verfasserin aut Yan Dang verfasserin aut Dawn E. Holmes verfasserin aut In Environmental Science and Ecotechnology Elsevier, 2021 6(2021), Seite 100092- (DE-627)1747161268 26664984 nnns volume:6 year:2021 pages:100092- https://doi.org/10.1016/j.ese.2021.100092 kostenfrei https://doaj.org/article/7415663cca38486394d992fc113738d5 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666498421000156 kostenfrei https://doaj.org/toc/2666-4984 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 6 2021 100092-
allfieldsSound	10.1016/j.ese.2021.100092 doi (DE-627)DOAJ072371277 (DE-599)DOAJ7415663cca38486394d992fc113738d5 DE-627 ger DE-627 rakwb eng GE1-350 TD1-1066 Pengsong Li verfasserin aut Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples. Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering Yumingzi Wang verfasserin aut Xin Yuan verfasserin aut Xinying Liu verfasserin aut Chunmao Liu verfasserin aut Xiaofen Fu verfasserin aut Dezhi Sun verfasserin aut Yan Dang verfasserin aut Dawn E. Holmes verfasserin aut In Environmental Science and Ecotechnology Elsevier, 2021 6(2021), Seite 100092- (DE-627)1747161268 26664984 nnns volume:6 year:2021 pages:100092- https://doi.org/10.1016/j.ese.2021.100092 kostenfrei https://doaj.org/article/7415663cca38486394d992fc113738d5 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666498421000156 kostenfrei https://doaj.org/toc/2666-4984 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 6 2021 100092-
language	English
source	In Environmental Science and Ecotechnology 6(2021), Seite 100092- volume:6 year:2021 pages:100092-
sourceStr	In Environmental Science and Ecotechnology 6(2021), Seite 100092- volume:6 year:2021 pages:100092-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon Environmental sciences Environmental technology. Sanitary engineering
isfreeaccess_bool	true
container_title	Environmental Science and Ecotechnology
authorswithroles_txt_mv	Pengsong Li @@aut@@ Yumingzi Wang @@aut@@ Xin Yuan @@aut@@ Xinying Liu @@aut@@ Chunmao Liu @@aut@@ Xiaofen Fu @@aut@@ Dezhi Sun @@aut@@ Yan Dang @@aut@@ Dawn E. Holmes @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	1747161268
id	DOAJ072371277
language_de	englisch
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callnumber-first	G - Geography, Anthropology, Recreation
author	Pengsong Li
spellingShingle	Pengsong Li misc GE1-350 misc TD1-1066 misc Arsenic detection misc Whole-cell biosensor misc Geobacter sulfurreducens misc ars operon misc Environmental sciences misc Environmental technology. Sanitary engineering Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens
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topic_title	GE1-350 TD1-1066 Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens Arsenic detection Whole-cell biosensor Geobacter sulfurreducens ars operon
topic	misc GE1-350 misc TD1-1066 misc Arsenic detection misc Whole-cell biosensor misc Geobacter sulfurreducens misc ars operon misc Environmental sciences misc Environmental technology. Sanitary engineering
topic_unstemmed	misc GE1-350 misc TD1-1066 misc Arsenic detection misc Whole-cell biosensor misc Geobacter sulfurreducens misc ars operon misc Environmental sciences misc Environmental technology. Sanitary engineering
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title	Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens
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title_full	Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens
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author_browse	Pengsong Li Yumingzi Wang Xin Yuan Xinying Liu Chunmao Liu Xiaofen Fu Dezhi Sun Yan Dang Dawn E. Holmes
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title_sort	development of a whole-cell biosensor based on an arsr-pars regulatory circuit from geobacter sulfurreducens
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title_auth	Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens
abstract	In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.
abstractGer	In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.
abstract_unstemmed	In this study, an Escherichia coli (E. coli) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from Geobacter sulfurreducens. E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. This study examines the potential applications of biosensors constructed with Geobacter ArsR-Pars regulatory circuits and provides a rapid and cost-effective tool that can be used for arsenic detection in water samples.
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title_short	Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens
url	https://doi.org/10.1016/j.ese.2021.100092 https://doaj.org/article/7415663cca38486394d992fc113738d5 http://www.sciencedirect.com/science/article/pii/S2666498421000156 https://doaj.org/toc/2666-4984
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E. coli cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the Geobacter arsR1 promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03–0.1 μM (2.25–7.5 μg/l). These values were well below World Health Organization’s drinking water quality standard, which is 10 μg/l. The feasibility of using this whole-cell biosensor to detect arsenic in water samples, such as arsenic polluted tap water and landfill leachate was verified. The biosensor was determined to be just as sensitive as atomic fluorescence spectrometry. 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Holmes</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Environmental Science and Ecotechnology</subfield><subfield code="d">Elsevier, 2021</subfield><subfield code="g">6(2021), Seite 100092-</subfield><subfield code="w">(DE-627)1747161268</subfield><subfield code="x">26664984</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:6</subfield><subfield code="g">year:2021</subfield><subfield code="g">pages:100092-</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.ese.2021.100092</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/7415663cca38486394d992fc113738d5</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield 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Development of a whole-cell biosensor based on an ArsR-Pars regulatory circuit from Geobacter sulfurreducens

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