Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic...
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Autor*in:	Raniere M. Moura [verfasserIn] Arthur A. Melo [verfasserIn] Rômulo F. Carneiro [verfasserIn] Cícera R.f. Rodrigues [verfasserIn] Plínio Delatorre [verfasserIn] Kyria S. Nascimento [verfasserIn] Silvana Saker-Sampaio [verfasserIn] Celso S. Nagano [verfasserIn] Benildo S. Cavada [verfasserIn] Alexandre H. Sampaio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	purificação hemólise citotoxicidade galactose

Übergeordnetes Werk:	In: Anais da Academia Brasileira de Ciências - Academia Brasileira de Ciências, 2004, 87(2015), 2, Seite 973-984
Übergeordnetes Werk:	volume:87 ; year:2015 ; number:2 ; pages:973-984

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/0001-3765201520140399

Katalog-ID:	DOAJ072447540

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520			\|a Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.
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allfields	10.1590/0001-3765201520140399 doi (DE-627)DOAJ072447540 (DE-599)DOAJe1d971871071475d9bb375319feca71a DE-627 ger DE-627 rakwb eng Raniere M. Moura verfasserin aut Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. purificação hemólise citotoxicidade galactose Science Q Arthur A. Melo verfasserin aut Rômulo F. Carneiro verfasserin aut Cícera R.f. Rodrigues verfasserin aut Plínio Delatorre verfasserin aut Kyria S. Nascimento verfasserin aut Silvana Saker-Sampaio verfasserin aut Celso S. Nagano verfasserin aut Benildo S. Cavada verfasserin aut Alexandre H. Sampaio verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 87(2015), 2, Seite 973-984 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns volume:87 year:2015 number:2 pages:973-984 https://doi.org/10.1590/0001-3765201520140399 kostenfrei https://doaj.org/article/e1d971871071475d9bb375319feca71a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 87 2015 2 973-984
spelling	10.1590/0001-3765201520140399 doi (DE-627)DOAJ072447540 (DE-599)DOAJe1d971871071475d9bb375319feca71a DE-627 ger DE-627 rakwb eng Raniere M. Moura verfasserin aut Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. purificação hemólise citotoxicidade galactose Science Q Arthur A. Melo verfasserin aut Rômulo F. Carneiro verfasserin aut Cícera R.f. Rodrigues verfasserin aut Plínio Delatorre verfasserin aut Kyria S. Nascimento verfasserin aut Silvana Saker-Sampaio verfasserin aut Celso S. Nagano verfasserin aut Benildo S. Cavada verfasserin aut Alexandre H. Sampaio verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 87(2015), 2, Seite 973-984 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns volume:87 year:2015 number:2 pages:973-984 https://doi.org/10.1590/0001-3765201520140399 kostenfrei https://doaj.org/article/e1d971871071475d9bb375319feca71a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 87 2015 2 973-984
allfields_unstemmed	10.1590/0001-3765201520140399 doi (DE-627)DOAJ072447540 (DE-599)DOAJe1d971871071475d9bb375319feca71a DE-627 ger DE-627 rakwb eng Raniere M. Moura verfasserin aut Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. purificação hemólise citotoxicidade galactose Science Q Arthur A. Melo verfasserin aut Rômulo F. Carneiro verfasserin aut Cícera R.f. Rodrigues verfasserin aut Plínio Delatorre verfasserin aut Kyria S. Nascimento verfasserin aut Silvana Saker-Sampaio verfasserin aut Celso S. Nagano verfasserin aut Benildo S. Cavada verfasserin aut Alexandre H. Sampaio verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 87(2015), 2, Seite 973-984 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns volume:87 year:2015 number:2 pages:973-984 https://doi.org/10.1590/0001-3765201520140399 kostenfrei https://doaj.org/article/e1d971871071475d9bb375319feca71a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 87 2015 2 973-984
allfieldsGer	10.1590/0001-3765201520140399 doi (DE-627)DOAJ072447540 (DE-599)DOAJe1d971871071475d9bb375319feca71a DE-627 ger DE-627 rakwb eng Raniere M. Moura verfasserin aut Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. purificação hemólise citotoxicidade galactose Science Q Arthur A. Melo verfasserin aut Rômulo F. Carneiro verfasserin aut Cícera R.f. Rodrigues verfasserin aut Plínio Delatorre verfasserin aut Kyria S. Nascimento verfasserin aut Silvana Saker-Sampaio verfasserin aut Celso S. Nagano verfasserin aut Benildo S. Cavada verfasserin aut Alexandre H. Sampaio verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 87(2015), 2, Seite 973-984 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns volume:87 year:2015 number:2 pages:973-984 https://doi.org/10.1590/0001-3765201520140399 kostenfrei https://doaj.org/article/e1d971871071475d9bb375319feca71a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 87 2015 2 973-984
allfieldsSound	10.1590/0001-3765201520140399 doi (DE-627)DOAJ072447540 (DE-599)DOAJe1d971871071475d9bb375319feca71a DE-627 ger DE-627 rakwb eng Raniere M. Moura verfasserin aut Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL. purificação hemólise citotoxicidade galactose Science Q Arthur A. Melo verfasserin aut Rômulo F. Carneiro verfasserin aut Cícera R.f. Rodrigues verfasserin aut Plínio Delatorre verfasserin aut Kyria S. Nascimento verfasserin aut Silvana Saker-Sampaio verfasserin aut Celso S. Nagano verfasserin aut Benildo S. Cavada verfasserin aut Alexandre H. Sampaio verfasserin aut In Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências, 2004 87(2015), 2, Seite 973-984 (DE-627)329269984 (DE-600)2046885-4 16782690 nnns volume:87 year:2015 number:2 pages:973-984 https://doi.org/10.1590/0001-3765201520140399 kostenfrei https://doaj.org/article/e1d971871071475d9bb375319feca71a kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en kostenfrei https://doaj.org/toc/1678-2690 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 87 2015 2 973-984
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source	In Anais da Academia Brasileira de Ciências 87(2015), 2, Seite 973-984 volume:87 year:2015 number:2 pages:973-984
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author	Raniere M. Moura
spellingShingle	Raniere M. Moura misc purificação misc hemólise misc citotoxicidade misc galactose misc Science misc Q Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
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topic_title	Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea purificação hemólise citotoxicidade galactose
topic	misc purificação misc hemólise misc citotoxicidade misc galactose misc Science misc Q
topic_unstemmed	misc purificação misc hemólise misc citotoxicidade misc galactose misc Science misc Q
topic_browse	misc purificação misc hemólise misc citotoxicidade misc galactose misc Science misc Q
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title	Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
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title_full	Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
author_sort	Raniere M. Moura
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author_browse	Raniere M. Moura Arthur A. Melo Rômulo F. Carneiro Cícera R.f. Rodrigues Plínio Delatorre Kyria S. Nascimento Silvana Saker-Sampaio Celso S. Nagano Benildo S. Cavada Alexandre H. Sampaio
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doi_str_mv	10.1590/0001-3765201520140399
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title_sort	hemagglutinating/hemolytic activities in extracts of marine invertebrates from the brazilian coast and isolation of two lectins from the marine sponge cliona varians and the sea cucumber holothuria grisea
title_auth	Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
abstract	Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.
abstractGer	Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.
abstract_unstemmed	Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC50 of 850.1 μg/mL, whereas HGL showed LC50 of 9.5 µg/mL.
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title_short	Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea
url	https://doi.org/10.1590/0001-3765201520140399 https://doaj.org/article/e1d971871071475d9bb375319feca71a http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200973&lng=en&tlng=en https://doaj.org/toc/1678-2690
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up_date	2024-07-04T01:06:46.834Z
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Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

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