Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina
The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
E. Scialfa [verfasserIn] J. Bolpe [verfasserIn] J. C. Bardón [verfasserIn] G. Ridao [verfasserIn] J. Gentile [verfasserIn] O. Gallicchio [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch ; Spanisch |
| Erschienen: |
2010 |
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| Übergeordnetes Werk: |
In: Revista Argentina de Microbiología - Elsevier España, 2017, 42(2010), 2, Seite 126-128 |
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| Übergeordnetes Werk: |
volume:42 ; year:2010 ; number:2 ; pages:126-128 |
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DOAJ072774371 |
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| 520 | |a The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. | ||
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(DE-627)DOAJ072774371 (DE-599)DOAJdb81e98e891c4221829e020f623ed151 DE-627 ger DE-627 rakwb eng spa RC109-216 QR1-502 E. Scialfa verfasserin aut Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. Leptospirosis R. norvegicus Transmisión al hombre Transmission to humans Infectious and parasitic diseases Microbiology J. Bolpe verfasserin aut J. C. Bardón verfasserin aut G. Ridao verfasserin aut J. Gentile verfasserin aut O. Gallicchio verfasserin aut In Revista Argentina de Microbiología Elsevier España, 2017 42(2010), 2, Seite 126-128 (DE-627)508330866 (DE-600)2223219-9 18517617 nnns volume:42 year:2010 number:2 pages:126-128 https://doaj.org/article/db81e98e891c4221829e020f623ed151 kostenfrei http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012 kostenfrei https://doaj.org/toc/0325-7541 Journal toc kostenfrei https://doaj.org/toc/1851-7617 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR 42 2010 2 126-128 |
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(DE-627)DOAJ072774371 (DE-599)DOAJdb81e98e891c4221829e020f623ed151 DE-627 ger DE-627 rakwb eng spa RC109-216 QR1-502 E. Scialfa verfasserin aut Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. Leptospirosis R. norvegicus Transmisión al hombre Transmission to humans Infectious and parasitic diseases Microbiology J. Bolpe verfasserin aut J. C. Bardón verfasserin aut G. Ridao verfasserin aut J. Gentile verfasserin aut O. Gallicchio verfasserin aut In Revista Argentina de Microbiología Elsevier España, 2017 42(2010), 2, Seite 126-128 (DE-627)508330866 (DE-600)2223219-9 18517617 nnns volume:42 year:2010 number:2 pages:126-128 https://doaj.org/article/db81e98e891c4221829e020f623ed151 kostenfrei http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012 kostenfrei https://doaj.org/toc/0325-7541 Journal toc kostenfrei https://doaj.org/toc/1851-7617 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR 42 2010 2 126-128 |
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(DE-627)DOAJ072774371 (DE-599)DOAJdb81e98e891c4221829e020f623ed151 DE-627 ger DE-627 rakwb eng spa RC109-216 QR1-502 E. Scialfa verfasserin aut Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. Leptospirosis R. norvegicus Transmisión al hombre Transmission to humans Infectious and parasitic diseases Microbiology J. Bolpe verfasserin aut J. C. Bardón verfasserin aut G. Ridao verfasserin aut J. Gentile verfasserin aut O. Gallicchio verfasserin aut In Revista Argentina de Microbiología Elsevier España, 2017 42(2010), 2, Seite 126-128 (DE-627)508330866 (DE-600)2223219-9 18517617 nnns volume:42 year:2010 number:2 pages:126-128 https://doaj.org/article/db81e98e891c4221829e020f623ed151 kostenfrei http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012 kostenfrei https://doaj.org/toc/0325-7541 Journal toc kostenfrei https://doaj.org/toc/1851-7617 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR 42 2010 2 126-128 |
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(DE-627)DOAJ072774371 (DE-599)DOAJdb81e98e891c4221829e020f623ed151 DE-627 ger DE-627 rakwb eng spa RC109-216 QR1-502 E. Scialfa verfasserin aut Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. Leptospirosis R. norvegicus Transmisión al hombre Transmission to humans Infectious and parasitic diseases Microbiology J. Bolpe verfasserin aut J. C. Bardón verfasserin aut G. Ridao verfasserin aut J. Gentile verfasserin aut O. Gallicchio verfasserin aut In Revista Argentina de Microbiología Elsevier España, 2017 42(2010), 2, Seite 126-128 (DE-627)508330866 (DE-600)2223219-9 18517617 nnns volume:42 year:2010 number:2 pages:126-128 https://doaj.org/article/db81e98e891c4221829e020f623ed151 kostenfrei http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012 kostenfrei https://doaj.org/toc/0325-7541 Journal toc kostenfrei https://doaj.org/toc/1851-7617 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR 42 2010 2 126-128 |
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(DE-627)DOAJ072774371 (DE-599)DOAJdb81e98e891c4221829e020f623ed151 DE-627 ger DE-627 rakwb eng spa RC109-216 QR1-502 E. Scialfa verfasserin aut Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. Leptospirosis R. norvegicus Transmisión al hombre Transmission to humans Infectious and parasitic diseases Microbiology J. Bolpe verfasserin aut J. C. Bardón verfasserin aut G. Ridao verfasserin aut J. Gentile verfasserin aut O. Gallicchio verfasserin aut In Revista Argentina de Microbiología Elsevier España, 2017 42(2010), 2, Seite 126-128 (DE-627)508330866 (DE-600)2223219-9 18517617 nnns volume:42 year:2010 number:2 pages:126-128 https://doaj.org/article/db81e98e891c4221829e020f623ed151 kostenfrei http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012 kostenfrei https://doaj.org/toc/0325-7541 Journal toc kostenfrei https://doaj.org/toc/1851-7617 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA AR 42 2010 2 126-128 |
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English Spanish |
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In Revista Argentina de Microbiología 42(2010), 2, Seite 126-128 volume:42 year:2010 number:2 pages:126-128 |
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isolation of leptospira interrogans from suburban rats in tandil, buenos aires, argentina aislamiento de leptospira interrogans de ratas suburbanas de tandil, buenos aires, argentina |
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Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina |
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The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. |
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The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. |
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The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis. |
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Scialfa</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation of Leptospira interrogans from suburban rats in Tandil, Buenos Aires, Argentina Aislamiento de Leptospira interrogans de ratas suburbanas de Tandil, Buenos Aires, Argentina</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2010</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The main objective of this study was to investigate the role of wild rodents as Leptospira spp. reservoirs in a suburban area of Tandil city, Buenos Aires province (Argentina), where a person had died due to pulmonary leptospirosis. The specific objectives were: to estimate the rodent density near the patient's home, to determine the serological prevalence and isolation of leptospirosis from wild rodents, and to identify the isolated strains. The area examined was a suburban neighbourhood in Tandil near the Langueyú stream, where the patient's house is located. Rattus norvegicus were trapped on the stream banks during two nights and a high capture rate (70%), was obtained. All rats (42) were examined serologically by the microscopic agglutination test (MAT), and 22 of them (52.3%) reacted with Leptospira serovars castellonis, canicola, grippotyphosa, icterohaemorrhagiae and hebdomadis at a titer of 1:50. The kidneys from 25 animals were cultured, and 24 isolates of L. interrogans (96%) were obtained. The isolated strains were identified as Icterohaemorrhagiae serogroup by MAT performed with rabbit hyperimmune reference sera. These findings showed a high density of suburban rodents highly infected with pathogenic leptospira, sharing environment in close contact with humans with evidence of leptospiral disease.<br<El objetivo general de este estudio fue investigar el papel de los roedores silvestres como reservorios de leptospiras en área suburbana de la ciudad de Tandil (Buenos Aires, Argentina), a raíz de un caso humano fatal de leptospirosis. Los objetivos específicos fueron estimar la densidad de roedores en el peridomicilio del paciente, determinar entre éstos la tasa de positividad a leptospirosis por serología, aislar dicho microorganismo e identificar las cepas aisladas. El área estudiada fue un barrio suburbano de Tandil próximo al arroyo Langueyú, donde residía el paciente. Se atraparon ejemplares de Rattus norvegicus sobre la orilla del arroyo durante dos noches, con una alta tasa de captura (70%). En las 42 ratas atrapadas se examinó la serología por el test de microaglutinación (TMA); 22 de ellas (52,3%) reaccionaron con los serovares castellonis, canicola, grippotyphosa, icterohaemorrhagiae y hebdomadis, a títulos de 1:50. El cultivo de tejido renal de 25 animales permitió obtener 24 aislamientos de L. interrogans (96%). Las cepas aisladas fueron identificadas como serogrupo Icterohaemorrhagiae mediante TMA utilizando suero hiperinmune de conejo. Estos hallazgos muestran una elevada densidad de roedores infectados con leptospiras patógenas en el área suburbana de la ciudad de Tandil, en estrecho contacto con población humana con evidencia de leptospirosis.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Leptospirosis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">R. norvegicus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transmisión al hombre</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Transmission to humans</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Microbiology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">J. Bolpe</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">J. C. Bardón</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">G. Ridao</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">J. Gentile</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">O. Gallicchio</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Revista Argentina de Microbiología</subfield><subfield code="d">Elsevier España, 2017</subfield><subfield code="g">42(2010), 2, Seite 126-128</subfield><subfield code="w">(DE-627)508330866</subfield><subfield code="w">(DE-600)2223219-9</subfield><subfield code="x">18517617</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:42</subfield><subfield code="g">year:2010</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:126-128</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/db81e98e891c4221829e020f623ed151</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-75412010000200012</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/0325-7541</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1851-7617</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">42</subfield><subfield code="j">2010</subfield><subfield code="e">2</subfield><subfield code="h">126-128</subfield></datafield></record></collection>
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