Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus

Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F....
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Gespeichert in:

Autor*in:	Jana Fránová [verfasserIn] Jaroslava Přibylová [verfasserIn] Igor Koloniuk [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission

Übergeordnetes Werk:	In: Viruses - MDPI AG, 2009, 11(2019), 11, p 982
Übergeordnetes Werk:	volume:11 ; year:2019 ; number:11, p 982

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/v11110982

Katalog-ID:	DOAJ072806060

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author_variant	j f jf j p jp i k ik
matchkey_str	article:19994915:2019----::oeuaadilgclhrceiainfnwtab
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callnumber-subject-code	QR
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allfields	10.3390/v11110982 doi (DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f DE-627 ger DE-627 rakwb eng QR1-502 Jana Fránová verfasserin aut Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions. novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology Jaroslava Přibylová verfasserin aut Igor Koloniuk verfasserin aut In Viruses MDPI AG, 2009 11(2019), 11, p 982 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:11 year:2019 number:11, p 982 https://doi.org/10.3390/v11110982 kostenfrei https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f kostenfrei https://www.mdpi.com/1999-4915/11/11/982 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 11, p 982
spelling	10.3390/v11110982 doi (DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f DE-627 ger DE-627 rakwb eng QR1-502 Jana Fránová verfasserin aut Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions. novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology Jaroslava Přibylová verfasserin aut Igor Koloniuk verfasserin aut In Viruses MDPI AG, 2009 11(2019), 11, p 982 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:11 year:2019 number:11, p 982 https://doi.org/10.3390/v11110982 kostenfrei https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f kostenfrei https://www.mdpi.com/1999-4915/11/11/982 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 11, p 982
allfields_unstemmed	10.3390/v11110982 doi (DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f DE-627 ger DE-627 rakwb eng QR1-502 Jana Fránová verfasserin aut Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions. novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology Jaroslava Přibylová verfasserin aut Igor Koloniuk verfasserin aut In Viruses MDPI AG, 2009 11(2019), 11, p 982 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:11 year:2019 number:11, p 982 https://doi.org/10.3390/v11110982 kostenfrei https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f kostenfrei https://www.mdpi.com/1999-4915/11/11/982 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 11, p 982
allfieldsGer	10.3390/v11110982 doi (DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f DE-627 ger DE-627 rakwb eng QR1-502 Jana Fránová verfasserin aut Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions. novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology Jaroslava Přibylová verfasserin aut Igor Koloniuk verfasserin aut In Viruses MDPI AG, 2009 11(2019), 11, p 982 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:11 year:2019 number:11, p 982 https://doi.org/10.3390/v11110982 kostenfrei https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f kostenfrei https://www.mdpi.com/1999-4915/11/11/982 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 11, p 982
allfieldsSound	10.3390/v11110982 doi (DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f DE-627 ger DE-627 rakwb eng QR1-502 Jana Fránová verfasserin aut Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions. novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology Jaroslava Přibylová verfasserin aut Igor Koloniuk verfasserin aut In Viruses MDPI AG, 2009 11(2019), 11, p 982 (DE-627)609775871 (DE-600)2516098-9 19994915 nnns volume:11 year:2019 number:11, p 982 https://doi.org/10.3390/v11110982 kostenfrei https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f kostenfrei https://www.mdpi.com/1999-4915/11/11/982 kostenfrei https://doaj.org/toc/1999-4915 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2019 11, p 982
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source	In Viruses 11(2019), 11, p 982 volume:11 year:2019 number:11, p 982
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topic_facet	novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp. <i<chaetosiphon fragaefolii</i< Microbiology
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topic_title	QR1-502 Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus novel cytorhabdovirus high-throughput sequencing aphid transmission mechanical inoculation grafting-mediated virus transmission <i<fragaria</i< spp <i<chaetosiphon fragaefolii</i<
topic	misc QR1-502 misc novel cytorhabdovirus misc high-throughput sequencing misc aphid transmission misc mechanical inoculation misc grafting-mediated virus transmission misc <i<fragaria</i< spp. misc <i<chaetosiphon fragaefolii</i< misc Microbiology
topic_unstemmed	misc QR1-502 misc novel cytorhabdovirus misc high-throughput sequencing misc aphid transmission misc mechanical inoculation misc grafting-mediated virus transmission misc <i<fragaria</i< spp. misc <i<chaetosiphon fragaefolii</i< misc Microbiology
topic_browse	misc QR1-502 misc novel cytorhabdovirus misc high-throughput sequencing misc aphid transmission misc mechanical inoculation misc grafting-mediated virus transmission misc <i<fragaria</i< spp. misc <i<chaetosiphon fragaefolii</i< misc Microbiology
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title	Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
ctrlnum	(DE-627)DOAJ072806060 (DE-599)DOAJ2ed6d6105b2948c096de88137b9ddd8f
title_full	Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
author_sort	Jana Fránová
journal	Viruses
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author_browse	Jana Fránová Jaroslava Přibylová Igor Koloniuk
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author-letter	Jana Fránová
doi_str_mv	10.3390/v11110982
author2-role	verfasserin
title_sort	molecular and biological characterization of a new strawberry cytorhabdovirus
callnumber	QR1-502
title_auth	Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
abstract	Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions.
abstractGer	Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions.
abstract_unstemmed	Virus diseases of strawberry present several complex problems. More than 25 viruses have been described in the genus <i<Fragaria</i< thus far. Here, we describe a novel rhabdovirus, tentatively named strawberry virus 1 (StrV-1), that infects <i<F. ananassa</i< and <i<F. vesca</i< plants. Genomic sequences of three distinct StrV-1 genotypes co-infecting a single <i<F. ananassa</i< host were obtained using combined Illumina and Ion Proton high-throughput sequencing. StrV-1 was transmitted to herbaceous plants via <i<Aphis fabae</i< and <i<A. ruborum</i<, further mechanically transmitted to <i<Nicotiana occidentalis</i< 37B and sub-inoculated to <i<N. benthamiana</i<, <i<N. benthamiana</i< DCL2/4i, <i<N.</i< <i<occidentalis</i< 37B, and <i<Physalis floridana</i< plants. Irregular chlorotic sectors on leaf blades and the multiplication of calyx leaves seem to be the diagnostic symptoms for StrV-1 on indexed <i<F. vesca</i< clones. StrV-1 was detected in asymptomatic grafted plants and in 49 out of 159 field strawberry samples via RT-PCR followed by Sanger sequencing. The bacilliform shape of the virions, which have a cytoplasm-limited distribution, their size, and phylogenetic relationships support the assignment of StrV-1 to a distinct species of the genus <i<Cytorhabdovirus</i<. <i<Acyrthosiphon malvae</i<, <i<A. fabae</i<, and <i<A. ruborum</i< were shown to transmit StrV-1 under experimental conditions.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	11, p 982
title_short	Molecular and Biological Characterization of a New Strawberry Cytorhabdovirus
url	https://doi.org/10.3390/v11110982 https://doaj.org/article/2ed6d6105b2948c096de88137b9ddd8f https://www.mdpi.com/1999-4915/11/11/982 https://doaj.org/toc/1999-4915
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