Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<

Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect...
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Autor*in:	Kang-Woon Lee [verfasserIn] Jae-Goo Kim [verfasserIn] Karpagam Veerappan [verfasserIn] Hoyong Chung [verfasserIn] Sathishkumar Natarajan [verfasserIn] Ki-Young Kim [verfasserIn] Junhyung Park [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	transcriptome antimicrobial peptide endangered species

Übergeordnetes Werk:	In: Insects - MDPI AG, 2011, 12(2021), 5, p 466
Übergeordnetes Werk:	volume:12 ; year:2021 ; number:5, p 466

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/insects12050466

Katalog-ID:	DOAJ073214663

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allfields	10.3390/insects12050466 doi (DE-627)DOAJ073214663 (DE-599)DOAJb55aa18df925452c8410c655516b48cc DE-627 ger DE-627 rakwb eng Kang-Woon Lee verfasserin aut Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis. <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q Jae-Goo Kim verfasserin aut Karpagam Veerappan verfasserin aut Hoyong Chung verfasserin aut Sathishkumar Natarajan verfasserin aut Ki-Young Kim verfasserin aut Junhyung Park verfasserin aut In Insects MDPI AG, 2011 12(2021), 5, p 466 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:12 year:2021 number:5, p 466 https://doi.org/10.3390/insects12050466 kostenfrei https://doaj.org/article/b55aa18df925452c8410c655516b48cc kostenfrei https://www.mdpi.com/2075-4450/12/5/466 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 5, p 466
spelling	10.3390/insects12050466 doi (DE-627)DOAJ073214663 (DE-599)DOAJb55aa18df925452c8410c655516b48cc DE-627 ger DE-627 rakwb eng Kang-Woon Lee verfasserin aut Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis. <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q Jae-Goo Kim verfasserin aut Karpagam Veerappan verfasserin aut Hoyong Chung verfasserin aut Sathishkumar Natarajan verfasserin aut Ki-Young Kim verfasserin aut Junhyung Park verfasserin aut In Insects MDPI AG, 2011 12(2021), 5, p 466 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:12 year:2021 number:5, p 466 https://doi.org/10.3390/insects12050466 kostenfrei https://doaj.org/article/b55aa18df925452c8410c655516b48cc kostenfrei https://www.mdpi.com/2075-4450/12/5/466 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 5, p 466
allfields_unstemmed	10.3390/insects12050466 doi (DE-627)DOAJ073214663 (DE-599)DOAJb55aa18df925452c8410c655516b48cc DE-627 ger DE-627 rakwb eng Kang-Woon Lee verfasserin aut Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis. <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q Jae-Goo Kim verfasserin aut Karpagam Veerappan verfasserin aut Hoyong Chung verfasserin aut Sathishkumar Natarajan verfasserin aut Ki-Young Kim verfasserin aut Junhyung Park verfasserin aut In Insects MDPI AG, 2011 12(2021), 5, p 466 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:12 year:2021 number:5, p 466 https://doi.org/10.3390/insects12050466 kostenfrei https://doaj.org/article/b55aa18df925452c8410c655516b48cc kostenfrei https://www.mdpi.com/2075-4450/12/5/466 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 5, p 466
allfieldsGer	10.3390/insects12050466 doi (DE-627)DOAJ073214663 (DE-599)DOAJb55aa18df925452c8410c655516b48cc DE-627 ger DE-627 rakwb eng Kang-Woon Lee verfasserin aut Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis. <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q Jae-Goo Kim verfasserin aut Karpagam Veerappan verfasserin aut Hoyong Chung verfasserin aut Sathishkumar Natarajan verfasserin aut Ki-Young Kim verfasserin aut Junhyung Park verfasserin aut In Insects MDPI AG, 2011 12(2021), 5, p 466 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:12 year:2021 number:5, p 466 https://doi.org/10.3390/insects12050466 kostenfrei https://doaj.org/article/b55aa18df925452c8410c655516b48cc kostenfrei https://www.mdpi.com/2075-4450/12/5/466 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 5, p 466
allfieldsSound	10.3390/insects12050466 doi (DE-627)DOAJ073214663 (DE-599)DOAJb55aa18df925452c8410c655516b48cc DE-627 ger DE-627 rakwb eng Kang-Woon Lee verfasserin aut Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis. <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q Jae-Goo Kim verfasserin aut Karpagam Veerappan verfasserin aut Hoyong Chung verfasserin aut Sathishkumar Natarajan verfasserin aut Ki-Young Kim verfasserin aut Junhyung Park verfasserin aut In Insects MDPI AG, 2011 12(2021), 5, p 466 (DE-627)718627121 (DE-600)2662247-6 20754450 nnns volume:12 year:2021 number:5, p 466 https://doi.org/10.3390/insects12050466 kostenfrei https://doaj.org/article/b55aa18df925452c8410c655516b48cc kostenfrei https://www.mdpi.com/2075-4450/12/5/466 kostenfrei https://doaj.org/toc/2075-4450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 5, p 466
language	English
source	In Insects 12(2021), 5, p 466 volume:12 year:2021 number:5, p 466
sourceStr	In Insects 12(2021), 5, p 466 volume:12 year:2021 number:5, p 466
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species Science Q
isfreeaccess_bool	true
container_title	Insects
authorswithroles_txt_mv	Kang-Woon Lee @@aut@@ Jae-Goo Kim @@aut@@ Karpagam Veerappan @@aut@@ Hoyong Chung @@aut@@ Sathishkumar Natarajan @@aut@@ Ki-Young Kim @@aut@@ Junhyung Park @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	718627121
id	DOAJ073214663
language_de	englisch
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author	Kang-Woon Lee
spellingShingle	Kang-Woon Lee misc <i<Parnassius bremeri</i< misc transcriptome misc antimicrobial peptide misc <i<Porphyromonas gingivalis</i< misc endangered species misc Science misc Q Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<
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topic_title	Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i< <i<Parnassius bremeri</i< transcriptome antimicrobial peptide <i<Porphyromonas gingivalis</i< endangered species
topic	misc <i<Parnassius bremeri</i< misc transcriptome misc antimicrobial peptide misc <i<Porphyromonas gingivalis</i< misc endangered species misc Science misc Q
topic_unstemmed	misc <i<Parnassius bremeri</i< misc transcriptome misc antimicrobial peptide misc <i<Porphyromonas gingivalis</i< misc endangered species misc Science misc Q
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title	Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<
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title_full	Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<
author_sort	Kang-Woon Lee
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author_browse	Kang-Woon Lee Jae-Goo Kim Karpagam Veerappan Hoyong Chung Sathishkumar Natarajan Ki-Young Kim Junhyung Park
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title_sort	utilizing red spotted apollo butterfly transcriptome to identify antimicrobial peptide candidates against <i<porphyromonas gingivalis</i<
title_auth	Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<
abstract	Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis.
abstractGer	Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis.
abstract_unstemmed	Classical antibiotics are the foremost treatment strategy against microbial infections. Overuse of this has led to the evolution of antimicrobial resistance. Antimicrobial peptides (AMPs) are natural defense elements present across many species including humans, insects, bacteria, and plants. Insect AMPs are our area of interest, because of their stronger abilities in host defense. We have deciphered AMPs from an endangered species <i<Parnassius bremeri</i<, commonly known as the red spotted apollo butterfly. It belongs to the second largest insect order Lepidoptera, comprised of butterflies and moths, and lives in the high altitudes of Russia, China, and Korea. We aimed at identifying the AMPs from the larvae stages. The rationale of choosing this stage is that the <i<P. bremeri</i< larvae development occurs at extremely low temperature conditions, which might serve as external stimuli for AMP production. RNA was isolated from larvae (L1 to L5) instar stages and subjected to next generation sequencing. The transcriptomes obtained were curated in in-silico pipelines. The peptides obtained were screened for requisite AMP physicochemical properties and in vitro antimicrobial activity. With the sequential screening and validation, we obtained fifteen candidate AMPs. One peptide TPS–032 showed promising antimicrobial activity against <i<Porphyromonas gingivalis</i<, a primary causative organism of periodontitis.
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container_issue	5, p 466
title_short	Utilizing Red Spotted Apollo Butterfly Transcriptome to Identify Antimicrobial Peptide Candidates against <i<Porphyromonas gingivalis</i<
url	https://doi.org/10.3390/insects12050466 https://doaj.org/article/b55aa18df925452c8410c655516b48cc https://www.mdpi.com/2075-4450/12/5/466 https://doaj.org/toc/2075-4450
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