New assessment of Anopheles vector species identification using MALDI-TOF MS

Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Cécile Nabet [verfasserIn] Abdoulaye K. Kone [verfasserIn] Abdoulaye K. Dia [verfasserIn] Moussa Sylla [verfasserIn] Magali Gautier [verfasserIn] Mohammed Yattara [verfasserIn] Mahamadou A. Thera [verfasserIn] Ousmane Faye [verfasserIn] Leo Braack [verfasserIn] Sylvie Manguin [verfasserIn] Abdoul H. Beavogui [verfasserIn] Ogobara Doumbo [verfasserIn] Frédérick Gay [verfasserIn] Renaud Piarroux [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head

Übergeordnetes Werk:	In: Malaria Journal - BMC, 2003, 20(2021), 1, Seite 16
Übergeordnetes Werk:	volume:20 ; year:2021 ; number:1 ; pages:16

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s12936-020-03557-2

Katalog-ID:	DOAJ073534153

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520			\|a Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.
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650		4	\|a Malaria vectors
650		4	\|a MALDI-TOF MS
650		4	\|a Taxonomic identification
650		4	\|a Anopheles gambiae
650		4	\|a Head
653		0	\|a Arctic medicine. Tropical medicine
653		0	\|a Infectious and parasitic diseases
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700	0		\|a Frédérick Gay \|e verfasserin \|4 aut
700	0		\|a Renaud Piarroux \|e verfasserin \|4 aut
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allfields	10.1186/s12936-020-03557-2 doi (DE-627)DOAJ073534153 (DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92 DE-627 ger DE-627 rakwb eng RC955-962 RC109-216 Cécile Nabet verfasserin aut New assessment of Anopheles vector species identification using MALDI-TOF MS 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases Abdoulaye K. Kone verfasserin aut Abdoulaye K. Dia verfasserin aut Moussa Sylla verfasserin aut Magali Gautier verfasserin aut Mohammed Yattara verfasserin aut Mahamadou A. Thera verfasserin aut Ousmane Faye verfasserin aut Leo Braack verfasserin aut Sylvie Manguin verfasserin aut Abdoul H. Beavogui verfasserin aut Ogobara Doumbo verfasserin aut Frédérick Gay verfasserin aut Renaud Piarroux verfasserin aut In Malaria Journal BMC, 2003 20(2021), 1, Seite 16 (DE-627)355986582 (DE-600)2091229-8 14752875 nnns volume:20 year:2021 number:1 pages:16 https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 kostenfrei https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/toc/1475-2875 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2021 1 16
spelling	10.1186/s12936-020-03557-2 doi (DE-627)DOAJ073534153 (DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92 DE-627 ger DE-627 rakwb eng RC955-962 RC109-216 Cécile Nabet verfasserin aut New assessment of Anopheles vector species identification using MALDI-TOF MS 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases Abdoulaye K. Kone verfasserin aut Abdoulaye K. Dia verfasserin aut Moussa Sylla verfasserin aut Magali Gautier verfasserin aut Mohammed Yattara verfasserin aut Mahamadou A. Thera verfasserin aut Ousmane Faye verfasserin aut Leo Braack verfasserin aut Sylvie Manguin verfasserin aut Abdoul H. Beavogui verfasserin aut Ogobara Doumbo verfasserin aut Frédérick Gay verfasserin aut Renaud Piarroux verfasserin aut In Malaria Journal BMC, 2003 20(2021), 1, Seite 16 (DE-627)355986582 (DE-600)2091229-8 14752875 nnns volume:20 year:2021 number:1 pages:16 https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 kostenfrei https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/toc/1475-2875 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2021 1 16
allfields_unstemmed	10.1186/s12936-020-03557-2 doi (DE-627)DOAJ073534153 (DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92 DE-627 ger DE-627 rakwb eng RC955-962 RC109-216 Cécile Nabet verfasserin aut New assessment of Anopheles vector species identification using MALDI-TOF MS 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases Abdoulaye K. Kone verfasserin aut Abdoulaye K. Dia verfasserin aut Moussa Sylla verfasserin aut Magali Gautier verfasserin aut Mohammed Yattara verfasserin aut Mahamadou A. Thera verfasserin aut Ousmane Faye verfasserin aut Leo Braack verfasserin aut Sylvie Manguin verfasserin aut Abdoul H. Beavogui verfasserin aut Ogobara Doumbo verfasserin aut Frédérick Gay verfasserin aut Renaud Piarroux verfasserin aut In Malaria Journal BMC, 2003 20(2021), 1, Seite 16 (DE-627)355986582 (DE-600)2091229-8 14752875 nnns volume:20 year:2021 number:1 pages:16 https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 kostenfrei https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/toc/1475-2875 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2021 1 16
allfieldsGer	10.1186/s12936-020-03557-2 doi (DE-627)DOAJ073534153 (DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92 DE-627 ger DE-627 rakwb eng RC955-962 RC109-216 Cécile Nabet verfasserin aut New assessment of Anopheles vector species identification using MALDI-TOF MS 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases Abdoulaye K. Kone verfasserin aut Abdoulaye K. Dia verfasserin aut Moussa Sylla verfasserin aut Magali Gautier verfasserin aut Mohammed Yattara verfasserin aut Mahamadou A. Thera verfasserin aut Ousmane Faye verfasserin aut Leo Braack verfasserin aut Sylvie Manguin verfasserin aut Abdoul H. Beavogui verfasserin aut Ogobara Doumbo verfasserin aut Frédérick Gay verfasserin aut Renaud Piarroux verfasserin aut In Malaria Journal BMC, 2003 20(2021), 1, Seite 16 (DE-627)355986582 (DE-600)2091229-8 14752875 nnns volume:20 year:2021 number:1 pages:16 https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 kostenfrei https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/toc/1475-2875 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2021 1 16
allfieldsSound	10.1186/s12936-020-03557-2 doi (DE-627)DOAJ073534153 (DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92 DE-627 ger DE-627 rakwb eng RC955-962 RC109-216 Cécile Nabet verfasserin aut New assessment of Anopheles vector species identification using MALDI-TOF MS 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases. Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases Abdoulaye K. Kone verfasserin aut Abdoulaye K. Dia verfasserin aut Moussa Sylla verfasserin aut Magali Gautier verfasserin aut Mohammed Yattara verfasserin aut Mahamadou A. Thera verfasserin aut Ousmane Faye verfasserin aut Leo Braack verfasserin aut Sylvie Manguin verfasserin aut Abdoul H. Beavogui verfasserin aut Ogobara Doumbo verfasserin aut Frédérick Gay verfasserin aut Renaud Piarroux verfasserin aut In Malaria Journal BMC, 2003 20(2021), 1, Seite 16 (DE-627)355986582 (DE-600)2091229-8 14752875 nnns volume:20 year:2021 number:1 pages:16 https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 kostenfrei https://doi.org/10.1186/s12936-020-03557-2 kostenfrei https://doaj.org/toc/1475-2875 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2021 1 16
language	English
source	In Malaria Journal 20(2021), 1, Seite 16 volume:20 year:2021 number:1 pages:16
sourceStr	In Malaria Journal 20(2021), 1, Seite 16 volume:20 year:2021 number:1 pages:16
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head Arctic medicine. Tropical medicine Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Malaria Journal
authorswithroles_txt_mv	Cécile Nabet @@aut@@ Abdoulaye K. Kone @@aut@@ Abdoulaye K. Dia @@aut@@ Moussa Sylla @@aut@@ Magali Gautier @@aut@@ Mohammed Yattara @@aut@@ Mahamadou A. Thera @@aut@@ Ousmane Faye @@aut@@ Leo Braack @@aut@@ Sylvie Manguin @@aut@@ Abdoul H. Beavogui @@aut@@ Ogobara Doumbo @@aut@@ Frédérick Gay @@aut@@ Renaud Piarroux @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	355986582
id	DOAJ073534153
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ073534153</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230309115549.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230228s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1186/s12936-020-03557-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ073534153</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ2003e79e13cc4c2182dfacadd00fda92</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC955-962</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC109-216</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Cécile Nabet</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">New assessment of Anopheles vector species identification using MALDI-TOF MS</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Anopheles</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Malaria vectors</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">MALDI-TOF MS</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Taxonomic identification</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Anopheles gambiae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Head</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Arctic medicine. Tropical medicine</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Abdoulaye K. 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callnumber-first	R - Medicine
author	Cécile Nabet
spellingShingle	Cécile Nabet misc RC955-962 misc RC109-216 misc Anopheles misc Malaria vectors misc MALDI-TOF MS misc Taxonomic identification misc Anopheles gambiae misc Head misc Arctic medicine. Tropical medicine misc Infectious and parasitic diseases New assessment of Anopheles vector species identification using MALDI-TOF MS
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topic_title	RC955-962 RC109-216 New assessment of Anopheles vector species identification using MALDI-TOF MS Anopheles Malaria vectors MALDI-TOF MS Taxonomic identification Anopheles gambiae Head
topic	misc RC955-962 misc RC109-216 misc Anopheles misc Malaria vectors misc MALDI-TOF MS misc Taxonomic identification misc Anopheles gambiae misc Head misc Arctic medicine. Tropical medicine misc Infectious and parasitic diseases
topic_unstemmed	misc RC955-962 misc RC109-216 misc Anopheles misc Malaria vectors misc MALDI-TOF MS misc Taxonomic identification misc Anopheles gambiae misc Head misc Arctic medicine. Tropical medicine misc Infectious and parasitic diseases
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title	New assessment of Anopheles vector species identification using MALDI-TOF MS
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title_full	New assessment of Anopheles vector species identification using MALDI-TOF MS
author_sort	Cécile Nabet
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author_browse	Cécile Nabet Abdoulaye K. Kone Abdoulaye K. Dia Moussa Sylla Magali Gautier Mohammed Yattara Mahamadou A. Thera Ousmane Faye Leo Braack Sylvie Manguin Abdoul H. Beavogui Ogobara Doumbo Frédérick Gay Renaud Piarroux
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title_sort	new assessment of anopheles vector species identification using maldi-tof ms
callnumber	RC955-962
title_auth	New assessment of Anopheles vector species identification using MALDI-TOF MS
abstract	Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.
abstractGer	Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.
abstract_unstemmed	Abstract Background Anopheles species identification is essential for an effective malaria vector control programme. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. Further research is needed to improve the standardization in order to share online spectral databases.
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title_short	New assessment of Anopheles vector species identification using MALDI-TOF MS
url	https://doi.org/10.1186/s12936-020-03557-2 https://doaj.org/article/2003e79e13cc4c2182dfacadd00fda92 https://doaj.org/toc/1475-2875
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author2	Abdoulaye K. Kone Abdoulaye K. Dia Moussa Sylla Magali Gautier Mohammed Yattara Mahamadou A. Thera Ousmane Faye Leo Braack Sylvie Manguin Abdoul H. Beavogui Ogobara Doumbo Frédérick Gay Renaud Piarroux
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Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has been developed to identify adult Anopheles species, using the legs or the cephalothorax. The protein repertoire from arthropods can vary according to compartment, but there is no general consensus regarding the anatomic part to be used. Methods To determine the body part of the Anopheles mosquitoes best suited for the identification of field specimens, a mass spectral library was generated with head, thorax with wings and legs of Anopheles gambiae, Anopheles arabiensis and Anopheles funestus obtained from reference centres. The MSL was evaluated using two independent panels of 52 and 40 An. gambiae field-collected in Mali and Guinea, respectively. Geographic variability was also tested using the panel from Mali and several databases containing added specimens from Mali and Senegal. Results Using the head and a database without specimens from the same field collection, the proportion of interpretable and correct identifications was significantly higher than using the other body parts at a threshold value of 1.7 (p < 0.0001). The thorax of engorged specimens was negatively impacted by the blood meal after frozen storage. The addition of specimens from Mali into the database significantly improved the results of Mali panel (p < 0.0001), which became comparable between head and legs. With higher identification scores, the using of the head will allow to decrease the number of technical replicates of protein extract per specimen, which represents a significant improvement for routine use of MALDI-TOF MS. Conclusions The using of the head of Anopheles may improve the performance of MALDI-TOF MS. Region-specific mass spectrum databases will have to be produced. 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