Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), conside...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Julio Cesar Muñoz Paredes [verfasserIn] Liliane Guimarães Oliveira [verfasserIn] Alexandre de Carvalho Braga [verfasserIn] Iara Maria Trevisol [verfasserIn] Paulo Michel Roehe [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch ; Portugiesisch

Erschienen:	1999

Schlagwörter:	Classical swine fever classical swine fever virus ELISA serology

Übergeordnetes Werk:	In: Pesquisa Veterinária Brasileira - Colégio Brasileiro de Patologia Animal (CBPA), 2004, 19(1999), 3-4, Seite 123-127
Übergeordnetes Werk:	volume:19 ; year:1999 ; number:3-4 ; pages:123-127

Links:	Link aufrufen Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/S0100-736X1999000300006

Katalog-ID:	DOAJ073619922

Internformat


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language	English Portuguese
source	In Pesquisa Veterinária Brasileira 19(1999), 3-4, Seite 123-127 volume:19 year:1999 number:3-4 pages:123-127
sourceStr	In Pesquisa Veterinária Brasileira 19(1999), 3-4, Seite 123-127 volume:19 year:1999 number:3-4 pages:123-127
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Classical swine fever classical swine fever virus ELISA serology Veterinary medicine
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authorswithroles_txt_mv	Julio Cesar Muñoz Paredes @@aut@@ Liliane Guimarães Oliveira @@aut@@ Alexandre de Carvalho Braga @@aut@@ Iara Maria Trevisol @@aut@@ Paulo Michel Roehe @@aut@@
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callnumber-first	S - Agriculture
author	Julio Cesar Muñoz Paredes
spellingShingle	Julio Cesar Muñoz Paredes misc SF600-1100 misc Classical swine fever misc classical swine fever virus misc ELISA misc serology misc Veterinary medicine Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
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topic_title	SF600-1100 Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever Classical swine fever classical swine fever virus ELISA serology
topic	misc SF600-1100 misc Classical swine fever misc classical swine fever virus misc ELISA misc serology misc Veterinary medicine
topic_unstemmed	misc SF600-1100 misc Classical swine fever misc classical swine fever virus misc ELISA misc serology misc Veterinary medicine
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title	Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
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title_full	Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
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author_browse	Julio Cesar Muñoz Paredes Liliane Guimarães Oliveira Alexandre de Carvalho Braga Iara Maria Trevisol Paulo Michel Roehe
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title_sort	development and standardization of an indirect elisa for the serological diagnosis of classical swine fever
callnumber	SF600-1100
title_auth	Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
abstract	An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.
abstractGer	An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.
abstract_unstemmed	An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.
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title_short	Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever
url	https://doi.org/10.1590/S0100-736X1999000300006 https://doaj.org/article/0b25c0e0805441268b44d5e2c5079074 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300006&tlng=en http://www.scielo.br/pdf/pvb/v19n3-4/0883.pdf https://doaj.org/toc/1678-5150
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author2	Liliane Guimarães Oliveira Alexandre de Carvalho Braga Iara Maria Trevisol Paulo Michel Roehe
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up_date	2024-07-03T18:42:29.307Z
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Development and standardization of an indirect ELISA for the serological diagnosis of classical swine fever

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