Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling
Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People&rsquo...
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Gespeichert in:
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Li D [verfasserIn] Wen S [verfasserIn] |
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E-Artikel |
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Englisch |
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2020 |
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lncrna linc00346； colorectal cancer； proliferation； apoptosis |
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| Übergeordnetes Werk: |
In: Cancer Management and Research - Dove Medical Press, 2009, (2020), Seite 4605-4614 |
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| Übergeordnetes Werk: |
year:2020 ; pages:4605-4614 |
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DOAJ073934577 |
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| 520 | |a Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 | ||
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(DE-627)DOAJ073934577 (DE-599)DOAJa4abf2b2010d428391615c3376f67d1e DE-627 ger DE-627 rakwb eng RC254-282 Li D verfasserin aut Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 lncrna linc00346； colorectal cancer； proliferation； apoptosis jak1/stat3 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen S verfasserin aut In Cancer Management and Research Dove Medical Press, 2009 (2020), Seite 4605-4614 (DE-627)606030840 (DE-600)2508013-1 11791322 nnns year:2020 pages:4605-4614 https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e kostenfrei https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR kostenfrei https://doaj.org/toc/1179-1322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 4605-4614 |
| spelling |
(DE-627)DOAJ073934577 (DE-599)DOAJa4abf2b2010d428391615c3376f67d1e DE-627 ger DE-627 rakwb eng RC254-282 Li D verfasserin aut Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 lncrna linc00346； colorectal cancer； proliferation； apoptosis jak1/stat3 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen S verfasserin aut In Cancer Management and Research Dove Medical Press, 2009 (2020), Seite 4605-4614 (DE-627)606030840 (DE-600)2508013-1 11791322 nnns year:2020 pages:4605-4614 https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e kostenfrei https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR kostenfrei https://doaj.org/toc/1179-1322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 4605-4614 |
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(DE-627)DOAJ073934577 (DE-599)DOAJa4abf2b2010d428391615c3376f67d1e DE-627 ger DE-627 rakwb eng RC254-282 Li D verfasserin aut Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 lncrna linc00346； colorectal cancer； proliferation； apoptosis jak1/stat3 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen S verfasserin aut In Cancer Management and Research Dove Medical Press, 2009 (2020), Seite 4605-4614 (DE-627)606030840 (DE-600)2508013-1 11791322 nnns year:2020 pages:4605-4614 https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e kostenfrei https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR kostenfrei https://doaj.org/toc/1179-1322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 4605-4614 |
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(DE-627)DOAJ073934577 (DE-599)DOAJa4abf2b2010d428391615c3376f67d1e DE-627 ger DE-627 rakwb eng RC254-282 Li D verfasserin aut Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 lncrna linc00346； colorectal cancer； proliferation； apoptosis jak1/stat3 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen S verfasserin aut In Cancer Management and Research Dove Medical Press, 2009 (2020), Seite 4605-4614 (DE-627)606030840 (DE-600)2508013-1 11791322 nnns year:2020 pages:4605-4614 https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e kostenfrei https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR kostenfrei https://doaj.org/toc/1179-1322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 4605-4614 |
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(DE-627)DOAJ073934577 (DE-599)DOAJa4abf2b2010d428391615c3376f67d1e DE-627 ger DE-627 rakwb eng RC254-282 Li D verfasserin aut Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 lncrna linc00346； colorectal cancer； proliferation； apoptosis jak1/stat3 Neoplasms. Tumors. Oncology. Including cancer and carcinogens Wen S verfasserin aut In Cancer Management and Research Dove Medical Press, 2009 (2020), Seite 4605-4614 (DE-627)606030840 (DE-600)2508013-1 11791322 nnns year:2020 pages:4605-4614 https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e kostenfrei https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR kostenfrei https://doaj.org/toc/1179-1322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2020 4605-4614 |
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LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. 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Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling |
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Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling |
| abstract |
Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 |
| abstractGer |
Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 |
| abstract_unstemmed |
Dan Li,1 Shuang Wen2 1Department of Pathology, Tianjin Baodi Hospital, Baodi Clinical College of Tianjin Medical University, Tianjin City 301800, People’s Republic of China; 2Department of Pathology, The Friendship Hospital of Dalian, Dalian City, Liaoning Province 116000, People’s Republic of ChinaCorrespondence: Shuang WenDepartment of Pathology, The Friendship Hospital of Dalian, No. 8, Sanba Square, Zhongshan District, Dalian City, Liaoning Province 116000, People’s Republic of ChinaTel +86-0411-82718822Email wenshuang1680163.comPurpose: The study was aimed to investigate the effect and mechanism of lncRNA LINC00346 on cell proliferation and apoptosis of colorectal cancer (CRC).Methods: The expression of lncRNA LINC00346 in CRC tissues and cells was detected by qRT-PCR. LINC00346 was overexpressed and silenced in HT29 and LoVo cells by the transfection of pcDNA-LINC00346 and si-LINC00346. The proliferation of CRC cells was detected by CCK-8 and colony-formation assay. The apoptosis was detected by flow cytometry assay. The expression of apoptosis-associated proteins (Caspase-3, Bcl-2, Bax) and JAK1/STAT3 signaling-associated proteins (JAK1, STAT3, p-JAK1, p-STAT3) was detected by Western blot. The tumor growth was detected in mice subcutaneous injected with transfected HT29 cells.Results: LINC00346 was significantly upregulated in CRC tissues and cells. Overexpression of LINC00346 significantly increased the OD450 values, number of colonies, decreased the apoptosis rate, upregulated Bcl-2, and downregulated Caspase-3 and Bax in HT29 and LoVo cells. Knockdown of LINC00346 exerted opposite results of proliferation and apoptosis on HT29 and LoVo cells. The expression levels of JAK1/JAK1 and p-STAT3/STAT3 were upregulated by LINC00346 overexpression. Tofacitinib (JAK1 inhibitor) reversed the tumor-promoting effect of LINC00346 overexpression on CRC cells. In vivo experiments further validated that LINC00346 overexpression promoted the growth of CRC xenograft tumors.Conclusion: LncRNA LINC00346 promoted the proliferation and inhibited the apoptosis of CRC cells through activating JAK1/STAT3 signaling.Keywords: LncRNA LINC00346, colorectal cancer, proliferation, apoptosis, JAK1/STAT3 |
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| title_short |
Silencing of lncRNA LINC00346 Inhibits the Proliferation and Promotes the Apoptosis of Colorectal Cancer Cells Through Inhibiting JAK1/STAT3 Signaling |
| url |
https://doaj.org/article/a4abf2b2010d428391615c3376f67d1e https://www.dovepress.com/silencing-of-lncrna-linc00346-inhibits-the-proliferation-and-promotes--peer-reviewed-article-CMAR https://doaj.org/toc/1179-1322 |
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2024-07-03T20:25:29.475Z |
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