Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells

Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and cons...
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Autor*in:	Sacha Horn [verfasserIn] Manuel Ritter [verfasserIn] Kathrin Arndts [verfasserIn] Dennis Borrero-Wolff [verfasserIn] Anna Wiszniewsky [verfasserIn] Linda Batsa Debrah [verfasserIn] Alexander Y. Debrah [verfasserIn] Jubin Osei-Mensah [verfasserIn] Mkunde Chachage [verfasserIn] Achim Hoerauf [verfasserIn] Inge Kroidl [verfasserIn] Laura E. Layland [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema

Übergeordnetes Werk:	In: Frontiers in Cellular and Infection Microbiology - Frontiers Media S.A., 2016, 11(2022)
Übergeordnetes Werk:	volume:11 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fcimb.2021.767306

Katalog-ID:	DOAJ074110926

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520			\|a Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures.
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allfields	10.3389/fcimb.2021.767306 doi (DE-627)DOAJ074110926 (DE-599)DOAJ2f36e528e88e4d198e9b2c898011f0b5 DE-627 ger DE-627 rakwb eng QR1-502 Sacha Horn verfasserin aut Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures. filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology Manuel Ritter verfasserin aut Kathrin Arndts verfasserin aut Dennis Borrero-Wolff verfasserin aut Anna Wiszniewsky verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Jubin Osei-Mensah verfasserin aut Jubin Osei-Mensah verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Inge Kroidl verfasserin aut Inge Kroidl verfasserin aut Laura E. Layland verfasserin aut Laura E. Layland verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 11(2022) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:11 year:2022 https://doi.org/10.3389/fcimb.2021.767306 kostenfrei https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 kostenfrei https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022
spelling	10.3389/fcimb.2021.767306 doi (DE-627)DOAJ074110926 (DE-599)DOAJ2f36e528e88e4d198e9b2c898011f0b5 DE-627 ger DE-627 rakwb eng QR1-502 Sacha Horn verfasserin aut Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures. filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology Manuel Ritter verfasserin aut Kathrin Arndts verfasserin aut Dennis Borrero-Wolff verfasserin aut Anna Wiszniewsky verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Jubin Osei-Mensah verfasserin aut Jubin Osei-Mensah verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Inge Kroidl verfasserin aut Inge Kroidl verfasserin aut Laura E. Layland verfasserin aut Laura E. Layland verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 11(2022) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:11 year:2022 https://doi.org/10.3389/fcimb.2021.767306 kostenfrei https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 kostenfrei https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022
allfields_unstemmed	10.3389/fcimb.2021.767306 doi (DE-627)DOAJ074110926 (DE-599)DOAJ2f36e528e88e4d198e9b2c898011f0b5 DE-627 ger DE-627 rakwb eng QR1-502 Sacha Horn verfasserin aut Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures. filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology Manuel Ritter verfasserin aut Kathrin Arndts verfasserin aut Dennis Borrero-Wolff verfasserin aut Anna Wiszniewsky verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Jubin Osei-Mensah verfasserin aut Jubin Osei-Mensah verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Inge Kroidl verfasserin aut Inge Kroidl verfasserin aut Laura E. Layland verfasserin aut Laura E. Layland verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 11(2022) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:11 year:2022 https://doi.org/10.3389/fcimb.2021.767306 kostenfrei https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 kostenfrei https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022
allfieldsGer	10.3389/fcimb.2021.767306 doi (DE-627)DOAJ074110926 (DE-599)DOAJ2f36e528e88e4d198e9b2c898011f0b5 DE-627 ger DE-627 rakwb eng QR1-502 Sacha Horn verfasserin aut Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures. filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology Manuel Ritter verfasserin aut Kathrin Arndts verfasserin aut Dennis Borrero-Wolff verfasserin aut Anna Wiszniewsky verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Jubin Osei-Mensah verfasserin aut Jubin Osei-Mensah verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Inge Kroidl verfasserin aut Inge Kroidl verfasserin aut Laura E. Layland verfasserin aut Laura E. Layland verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 11(2022) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:11 year:2022 https://doi.org/10.3389/fcimb.2021.767306 kostenfrei https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 kostenfrei https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022
allfieldsSound	10.3389/fcimb.2021.767306 doi (DE-627)DOAJ074110926 (DE-599)DOAJ2f36e528e88e4d198e9b2c898011f0b5 DE-627 ger DE-627 rakwb eng QR1-502 Sacha Horn verfasserin aut Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures. filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology Manuel Ritter verfasserin aut Kathrin Arndts verfasserin aut Dennis Borrero-Wolff verfasserin aut Anna Wiszniewsky verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Linda Batsa Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Alexander Y. Debrah verfasserin aut Jubin Osei-Mensah verfasserin aut Jubin Osei-Mensah verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Mkunde Chachage verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Achim Hoerauf verfasserin aut Inge Kroidl verfasserin aut Inge Kroidl verfasserin aut Laura E. Layland verfasserin aut Laura E. Layland verfasserin aut In Frontiers in Cellular and Infection Microbiology Frontiers Media S.A., 2016 11(2022) (DE-627)664968554 (DE-600)2619676-1 22352988 nnns volume:11 year:2022 https://doi.org/10.3389/fcimb.2021.767306 kostenfrei https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 kostenfrei https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full kostenfrei https://doaj.org/toc/2235-2988 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022
language	English
source	In Frontiers in Cellular and Infection Microbiology 11(2022) volume:11 year:2022
sourceStr	In Frontiers in Cellular and Infection Microbiology 11(2022) volume:11 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema Microbiology
isfreeaccess_bool	true
container_title	Frontiers in Cellular and Infection Microbiology
authorswithroles_txt_mv	Sacha Horn @@aut@@ Manuel Ritter @@aut@@ Kathrin Arndts @@aut@@ Dennis Borrero-Wolff @@aut@@ Anna Wiszniewsky @@aut@@ Linda Batsa Debrah @@aut@@ Alexander Y. Debrah @@aut@@ Jubin Osei-Mensah @@aut@@ Mkunde Chachage @@aut@@ Achim Hoerauf @@aut@@ Inge Kroidl @@aut@@ Laura E. Layland @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	664968554
id	DOAJ074110926
language_de	englisch
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callnumber-first	Q - Science
author	Sacha Horn
spellingShingle	Sacha Horn misc QR1-502 misc filariae misc CD4+ T cell exhaustion misc Wuchereria bancrofti infection misc lymphatic filariasis misc immune modulation misc lymphedema misc Microbiology Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells
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topic_title	QR1-502 Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells filariae CD4+ T cell exhaustion Wuchereria bancrofti infection lymphatic filariasis immune modulation lymphedema
topic	misc QR1-502 misc filariae misc CD4+ T cell exhaustion misc Wuchereria bancrofti infection misc lymphatic filariasis misc immune modulation misc lymphedema misc Microbiology
topic_unstemmed	misc QR1-502 misc filariae misc CD4+ T cell exhaustion misc Wuchereria bancrofti infection misc lymphatic filariasis misc immune modulation misc lymphedema misc Microbiology
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title	Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells
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title_full	Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells
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author_browse	Sacha Horn Manuel Ritter Kathrin Arndts Dennis Borrero-Wolff Anna Wiszniewsky Linda Batsa Debrah Alexander Y. Debrah Jubin Osei-Mensah Mkunde Chachage Achim Hoerauf Inge Kroidl Laura E. Layland
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title_sort	filarial lymphedema patients are characterized by exhausted cd4+ t cells
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title_auth	Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells
abstract	Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures.
abstractGer	Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures.
abstract_unstemmed	Worldwide, more than 200 million people are infected with filariae which can cause severe symptoms leading to reduced quality of life and contribute to disability-adjusted life years (DALYs). In particular, lymphatic filariasis (LF) caused by Wuchereria bancrofti can lead to lymphedema (LE) and consequently presents a serious health problem. To understand why only a fraction of the infected individuals develop pathology, it is essential to understand how filariae regulate host immunity. The central role of T cells for immunity against filariae has been shown in several studies. However, there is little knowledge about T cell exhaustion, which causes T cell dysfunction and impaired immune responses, in this group of individuals. Recently, we showed that LE patients from Ghana harbor distinct patterns of exhausted effector and memory CD8+ T cell subsets. Based on these findings, we now characterized CD4+ T cell subsets from the same Ghanaian patient cohort by analyzing distinct markers within a 13-colour flow cytometry panel. We revealed that LE patients had increased frequencies of CD4+ T cells expressing exhaustion-associated receptors such as KLRG-1, TIM-3 and PD-1 compared to healthy endemic normal and W. bancrofti-infected individuals. Moreover, CD4+ T cells in LE patients were characterized by distinct co-expression patterns of inhibitory receptors. Collectively with the previous findings on CD8+ T cell exhaustion patterns, the data shown here demonstrates that filarial LE patients harbor distinct subsets of exhausted T cells. Thus, T cell exhaustion patterns in LE patients need attention especially in regards to susceptibility of concomitant infections and should be taken into consideration for LE management measures.
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title_short	Filarial Lymphedema Patients Are Characterized by Exhausted CD4+ T Cells
url	https://doi.org/10.3389/fcimb.2021.767306 https://doaj.org/article/2f36e528e88e4d198e9b2c898011f0b5 https://www.frontiersin.org/articles/10.3389/fcimb.2021.767306/full https://doaj.org/toc/2235-2988
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up_date	2024-07-03T21:23:29.950Z
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