Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway

In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the grow...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Jianjun Li [verfasserIn] Chuan Xiong [verfasserIn] Pan Xu [verfasserIn] Qiang Luo [verfasserIn] Ronggui Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway

Übergeordnetes Werk:	In: Bioengineered - Taylor & Francis Group, 2019, 12(2021), 1, Seite 402-413
Übergeordnetes Werk:	volume:12 ; year:2021 ; number:1 ; pages:402-413

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/21655979.2020.1868733

Katalog-ID:	DOAJ074223712

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520			\|a In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway.
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allfields	10.1080/21655979.2020.1868733 doi (DE-627)DOAJ074223712 (DE-599)DOAJ51da478d3871401fa2ec7f2ac9e99745 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Jianjun Li verfasserin aut Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology Chuan Xiong verfasserin aut Pan Xu verfasserin aut Qiang Luo verfasserin aut Ronggui Zhang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 12(2021), 1, Seite 402-413 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:12 year:2021 number:1 pages:402-413 https://doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 1 402-413
spelling	10.1080/21655979.2020.1868733 doi (DE-627)DOAJ074223712 (DE-599)DOAJ51da478d3871401fa2ec7f2ac9e99745 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Jianjun Li verfasserin aut Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology Chuan Xiong verfasserin aut Pan Xu verfasserin aut Qiang Luo verfasserin aut Ronggui Zhang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 12(2021), 1, Seite 402-413 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:12 year:2021 number:1 pages:402-413 https://doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 1 402-413
allfields_unstemmed	10.1080/21655979.2020.1868733 doi (DE-627)DOAJ074223712 (DE-599)DOAJ51da478d3871401fa2ec7f2ac9e99745 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Jianjun Li verfasserin aut Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology Chuan Xiong verfasserin aut Pan Xu verfasserin aut Qiang Luo verfasserin aut Ronggui Zhang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 12(2021), 1, Seite 402-413 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:12 year:2021 number:1 pages:402-413 https://doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 1 402-413
allfieldsGer	10.1080/21655979.2020.1868733 doi (DE-627)DOAJ074223712 (DE-599)DOAJ51da478d3871401fa2ec7f2ac9e99745 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Jianjun Li verfasserin aut Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology Chuan Xiong verfasserin aut Pan Xu verfasserin aut Qiang Luo verfasserin aut Ronggui Zhang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 12(2021), 1, Seite 402-413 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:12 year:2021 number:1 pages:402-413 https://doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 1 402-413
allfieldsSound	10.1080/21655979.2020.1868733 doi (DE-627)DOAJ074223712 (DE-599)DOAJ51da478d3871401fa2ec7f2ac9e99745 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Jianjun Li verfasserin aut Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology Chuan Xiong verfasserin aut Pan Xu verfasserin aut Qiang Luo verfasserin aut Ronggui Zhang verfasserin aut In Bioengineered Taylor & Francis Group, 2019 12(2021), 1, Seite 402-413 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:12 year:2021 number:1 pages:402-413 https://doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1868733 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2021 1 402-413
language	English
source	In Bioengineered 12(2021), 1, Seite 402-413 volume:12 year:2021 number:1 pages:402-413
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topic_facet	puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway Biotechnology
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authorswithroles_txt_mv	Jianjun Li @@aut@@ Chuan Xiong @@aut@@ Pan Xu @@aut@@ Qiang Luo @@aut@@ Ronggui Zhang @@aut@@
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callnumber-first	T - Technology
author	Jianjun Li
spellingShingle	Jianjun Li misc TP248.13-248.65 misc puerarin misc prostate cancer cells misc androgen-independent misc apoptosis misc keap1/nrf2/are signaling pathway misc Biotechnology Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway
authorStr	Jianjun Li
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topic_title	TP248.13-248.65 Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway puerarin prostate cancer cells androgen-independent apoptosis keap1/nrf2/are signaling pathway
topic	misc TP248.13-248.65 misc puerarin misc prostate cancer cells misc androgen-independent misc apoptosis misc keap1/nrf2/are signaling pathway misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc puerarin misc prostate cancer cells misc androgen-independent misc apoptosis misc keap1/nrf2/are signaling pathway misc Biotechnology
topic_browse	misc TP248.13-248.65 misc puerarin misc prostate cancer cells misc androgen-independent misc apoptosis misc keap1/nrf2/are signaling pathway misc Biotechnology
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title	Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway
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title_full	Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway
author_sort	Jianjun Li
journal	Bioengineered
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author_browse	Jianjun Li Chuan Xiong Pan Xu Qiang Luo Ronggui Zhang
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title_sort	puerarin induces apoptosis in prostate cancer cells via inactivation of the keap1/nrf2/are signaling pathway
callnumber	TP248.13-248.65
title_auth	Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway
abstract	In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway.
abstractGer	In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway.
abstract_unstemmed	In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 μM, 5 μM, and 10 μM) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 μM at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1β and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway.
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title_short	Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway
url	https://doi.org/10.1080/21655979.2020.1868733 https://doaj.org/article/51da478d3871401fa2ec7f2ac9e99745 http://dx.doi.org/10.1080/21655979.2020.1868733 https://doaj.org/toc/2165-5979 https://doaj.org/toc/2165-5987
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up_date	2024-07-03T22:00:37.283Z
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Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway

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