A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic

Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we...
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Gespeichert in:

Autor*in:	Anjali Patond [verfasserIn] Rahul Narang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c

Übergeordnetes Werk:	In: Journal of Mahatma Gandhi Institute of Medical Sciences - Wolters Kluwer Medknow Publications, 2018, 25(2020), 2, Seite 80-85
Übergeordnetes Werk:	volume:25 ; year:2020 ; number:2 ; pages:80-85

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4103/jmgims.jmgims_48_20

Katalog-ID:	DOAJ07422851X

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520			\|a Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic.
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912			\|a GBV_ILN_602
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2014
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allfields	10.4103/jmgims.jmgims_48_20 doi (DE-627)DOAJ07422851X (DE-599)DOAJ358121de99394a0c910f3793c9e59f69 DE-627 ger DE-627 rakwb eng RZ201-999 Anjali Patond verfasserin aut A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic. covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c Medicine R Other systems of medicine Rahul Narang verfasserin aut In Journal of Mahatma Gandhi Institute of Medical Sciences Wolters Kluwer Medknow Publications, 2018 25(2020), 2, Seite 80-85 (DE-627)77127498X (DE-600)2738506-1 23471948 nnns volume:25 year:2020 number:2 pages:80-85 https://doi.org/10.4103/jmgims.jmgims_48_20 kostenfrei https://doaj.org/article/358121de99394a0c910f3793c9e59f69 kostenfrei http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond kostenfrei https://doaj.org/toc/0971-9903 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 2 80-85
spelling	10.4103/jmgims.jmgims_48_20 doi (DE-627)DOAJ07422851X (DE-599)DOAJ358121de99394a0c910f3793c9e59f69 DE-627 ger DE-627 rakwb eng RZ201-999 Anjali Patond verfasserin aut A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic. covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c Medicine R Other systems of medicine Rahul Narang verfasserin aut In Journal of Mahatma Gandhi Institute of Medical Sciences Wolters Kluwer Medknow Publications, 2018 25(2020), 2, Seite 80-85 (DE-627)77127498X (DE-600)2738506-1 23471948 nnns volume:25 year:2020 number:2 pages:80-85 https://doi.org/10.4103/jmgims.jmgims_48_20 kostenfrei https://doaj.org/article/358121de99394a0c910f3793c9e59f69 kostenfrei http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond kostenfrei https://doaj.org/toc/0971-9903 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 2 80-85
allfields_unstemmed	10.4103/jmgims.jmgims_48_20 doi (DE-627)DOAJ07422851X (DE-599)DOAJ358121de99394a0c910f3793c9e59f69 DE-627 ger DE-627 rakwb eng RZ201-999 Anjali Patond verfasserin aut A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic. covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c Medicine R Other systems of medicine Rahul Narang verfasserin aut In Journal of Mahatma Gandhi Institute of Medical Sciences Wolters Kluwer Medknow Publications, 2018 25(2020), 2, Seite 80-85 (DE-627)77127498X (DE-600)2738506-1 23471948 nnns volume:25 year:2020 number:2 pages:80-85 https://doi.org/10.4103/jmgims.jmgims_48_20 kostenfrei https://doaj.org/article/358121de99394a0c910f3793c9e59f69 kostenfrei http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond kostenfrei https://doaj.org/toc/0971-9903 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 2 80-85
allfieldsGer	10.4103/jmgims.jmgims_48_20 doi (DE-627)DOAJ07422851X (DE-599)DOAJ358121de99394a0c910f3793c9e59f69 DE-627 ger DE-627 rakwb eng RZ201-999 Anjali Patond verfasserin aut A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic. covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c Medicine R Other systems of medicine Rahul Narang verfasserin aut In Journal of Mahatma Gandhi Institute of Medical Sciences Wolters Kluwer Medknow Publications, 2018 25(2020), 2, Seite 80-85 (DE-627)77127498X (DE-600)2738506-1 23471948 nnns volume:25 year:2020 number:2 pages:80-85 https://doi.org/10.4103/jmgims.jmgims_48_20 kostenfrei https://doaj.org/article/358121de99394a0c910f3793c9e59f69 kostenfrei http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond kostenfrei https://doaj.org/toc/0971-9903 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 2 80-85
allfieldsSound	10.4103/jmgims.jmgims_48_20 doi (DE-627)DOAJ07422851X (DE-599)DOAJ358121de99394a0c910f3793c9e59f69 DE-627 ger DE-627 rakwb eng RZ201-999 Anjali Patond verfasserin aut A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic. covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c Medicine R Other systems of medicine Rahul Narang verfasserin aut In Journal of Mahatma Gandhi Institute of Medical Sciences Wolters Kluwer Medknow Publications, 2018 25(2020), 2, Seite 80-85 (DE-627)77127498X (DE-600)2738506-1 23471948 nnns volume:25 year:2020 number:2 pages:80-85 https://doi.org/10.4103/jmgims.jmgims_48_20 kostenfrei https://doaj.org/article/358121de99394a0c910f3793c9e59f69 kostenfrei http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond kostenfrei https://doaj.org/toc/0971-9903 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 2 80-85
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callnumber-first	R - Medicine
author	Anjali Patond
spellingShingle	Anjali Patond misc RZ201-999 misc covid-19 misc decontamination misc n95 filtering face-piece respirators misc pandemic misc ultraviolet c misc Medicine misc R misc Other systems of medicine A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic
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topic_title	RZ201-999 A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic covid-19 decontamination n95 filtering face-piece respirators pandemic ultraviolet c
topic	misc RZ201-999 misc covid-19 misc decontamination misc n95 filtering face-piece respirators misc pandemic misc ultraviolet c misc Medicine misc R misc Other systems of medicine
topic_unstemmed	misc RZ201-999 misc covid-19 misc decontamination misc n95 filtering face-piece respirators misc pandemic misc ultraviolet c misc Medicine misc R misc Other systems of medicine
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title	A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic
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title_full	A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic
author_sort	Anjali Patond
journal	Journal of Mahatma Gandhi Institute of Medical Sciences
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title_sort	low cost ingenious approach for ultraviolet decontamination of n95 filtering face-piece respirators to deal with dwindling supply during the covid-19 pandemic
callnumber	RZ201-999
title_auth	A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic
abstract	Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic.
abstractGer	Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic.
abstract_unstemmed	Introduction: COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus-2 is rapidly evolving and hospitals are facing the issue of shortage of personal protective equipment (PPE) due to stringent requirements of PPE use. For reuse of N95 filtering face-piece respirators (N95FFR), we prepared an ingenious cabinet with ultraviolet light C (UV-C) using scarce material available during the lockdown period. Materials and Methods: Since like many other microbiology laboratories in medical colleges, we did not have access to viruses, we could not test our product with them. We thus tested the efficacy of the cabinet to decontaminate material using 0.5 McFarland standard broth of Escherichia coli 25922. The broth was exposed to UV-C inside the cabinet for 15 and 30 minutes in Petri dishes, with and without lids. The broth was subcultured on nutrient agar plates, both pre and post exposure. We also could not test integrity and static charge of the respirators; we relied on CDC data on the same. Results: It was observed that there was at least 4 log reduction (99.99%) in the number of viable E. coli on exposure to UV-C for 15 as well as 30 minutes. The plates with glass lids on did not show any reduction in number of viable bacilli. The reduction in number of E. coli was taken as surrogate marker for the reduction of ssRNA viruses. Conclusions: UV-C inside an ingeniously made cabinet can be used to decontaminate N95 FFR in exceptional circumstances of reduced supply under lockdown conditions of a pandemic.
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title_short	A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic
url	https://doi.org/10.4103/jmgims.jmgims_48_20 https://doaj.org/article/358121de99394a0c910f3793c9e59f69 http://www.jmgims.co.in/article.asp?issn=0971-9903;year=2020;volume=25;issue=2;spage=80;epage=85;aulast=Patond https://doaj.org/toc/0971-9903
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A low cost ingenious approach for ultraviolet decontamination of N95 filtering face-piece respirators to deal with dwindling supply during the COVID-19 pandemic

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